Preparation of anti-nortestosterone antibodies and development of an indirect heterologous competitive enzyme-linked immunosorbent assay to detect nortestosterone residues in animal urine

Analytical Letters

Volumn 44, Issue 14, 2011, Pages 2373-2393

  Jiang, Jinqing ^a,b   Zhang, Haitang ^b   Li, Guangling ^b   Wang, Ziliang ^b   Wang, Jianhua ^a   Zhao, Hengzhang ^b  

^a NORTHWEST A F UNIVERSITY   (China)

^b HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

19 Nortestosterone; Animal urine; Artificial antigen; Detection; IcELISA; Immunological trait; Polyclonal antibody

Indexed keywords

ANIMALIA; BOVINAE; CANIS FAMILIARIS; EQUIDAE; ORYCTOLAGUS CUNICULUS;

EID: 80052452771     PISSN: 00032719     EISSN: 1532236X     Source Type: Journal    
DOI: 10.1080/00032719.2010.551694     Document Type: Article

References (25)

1. Axén, R., J. Porath, and S. Ernback. 1967. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature 214: 1302-1304.
2. Beier, R. C., L. C. Creemer, R. L. Ziprin, and D. J. Nisbet. 2005. Production and characterization of monoclonal antibodies against the antibiotic tilmicosin. J. Agric. Food Chem. 53: 9679-9688.
3. Beier, R. C., L. H. Ripley, C. R. Young, and C. M. Kaiser. 2001. Production, characterization, and cross-reactivity studies of monoclonal antibodies against the coccidiostat nicarbazin. J. Agric. Food Chem. 49: 4542-4552.
4. Brännvall, K., N. Bogdanovic, L. Korhonen, and D. Lindholm. 2005. 19-Nortestosterone influences neural stem cell proliferation and neurogenesis in the rat brain. Eur. J. Neurosci. 21: 871-878.
5. Bricout, V., and F. Wright. 2004. Update on nandrolone and norsteroids: How endogenous or xenobiotic are these substances? Eur. J. Appl. Physiol. 92: 1-12.
6. Cooper, J., W. Currie, and C. T. Elliott. 2001. Comparison of the efficiencies of enzymatic and chemical hydrolysis of (nortestosterone and diethylstilboestrol) glucuronides in bovine urine. J. Chromatogr. B 757: 221-227.
7. Daeseleire, E. A., A. de Guesquière, and C. H. Van Peteghem. 1992. Multiresidue analysis of anabolic agents in muscle tissues and urines of cattle by GC-MS. J. Chromatogr. Sci. 30: 409-414.
8. Draisci, R., L. Palleschi, E. Ferretti, L. Lucentini, and P. Cammarata. 2000. Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 870: 511-522.
9. Evrard, P., P. Gaspar, and G. Maghuin. 1986. A specific radioimmunoassay for the detection of 19-Nortestosterone residues in urine and plasma of cattle. J. Immunoassay 7: 353-363.
10. Hall, C. E., and S. Hungerford. 1982. Similarities and differences between effects of testosterone and 19-Nortestosterone in rats, with particular reference to hyper tensogenic potency. J. Steroid Biochem. 16: 581-585.
11. Houghton, E., and M. C. Dumasia. 1980. Studies related to the metabolism of anabolic steroids in the horse: the identification of some 16-oxygenated metabolites of 19-Nortestosterone. Xenobiotica 10: 381-390.
12. Kicman, A. T., and D. B. Gower. 2003. Anabolic steroids in sport: Biochemical, clinical and analytical perspectives. Ann. Clin. Biochem. 40: 321-356.
13. Liang, Y., X. J. Liu, Y. Liu, X. Y. Yu, and M. T. Fan. 2008. Synthesis of three haptens for the class-specific immunoassay of O,O-dimethyl organophosphorus pesticides and effect of hapten heterology on immunoassay sensitivity. Anal. Chim. Acta 615: 174-183.
14. Liu, L., C. Peng, Z. Jin, and C. Xu. 2007. Development and evaluation of a rapid lateral flow immunochromatographic strip assay for screening 19-Nortestosterone. Biomed. Chromatogr. 21: 861-866.
15. Masse, R., C. Ayotte, and R. Dugal. 1989. Studies on anabolic steroids. I. Integrated methodological approach to the gas chromatographic-mass spectrometric analysis of anabolic steroid metabolites in urine. J. Chromatogr. 489: 23-50.
16. Midgley, A. R., G. D. Niswender, and R. W. Rebar. 1969. Principles for the assessment of reliability of radioimmunoassay methods (precision, accuracy, sensitivity, specificity). Acta Endocrinol. Suppl. (Copenh.) 142: 163-184.
17. Oriundi, M. P., R. Angeletti, E. Bastiani, C. Nachtmann, K. E. Vanoosthuyze, and C. Van Peteghem. 1995. Screening of calf urine for 19-Nortestosterone: Matrix effect in some immunoassays. Analyst 120: 577-579.
18. Plocm, M. E., W. Haasnoot, R. J. Paulussen, G. D. van Bruchem, A. R. Hamers, R. Schilt, and F. A. Huf. 1991. Test strip enzyme immunoassays and the fast screening of nortestosterone and clenbuterol residues in urine samples at the parts per billion level. J. Chromatogr. 564: 413-427.
19. Popii, V., and G. Baumann. 2004. Laboratory measurement of growth hormone. Clin. Chim. Acta 350: 1-16.
20. Roda, A., A. C. Manetta, O. Portanti, M. Mirasoli, M. Guardigli, P. Pasini, and R. Lelli. 2003. A rapid and sensitive 384-well microtitre format chemiluminescent enzyme immunoassay for 19-Nortestosterone. Luminescence 18: 72-78.
21. Samuels, T. P., A. Nedderman, M. A. Seymour, and E. Houghton. 1998. Study of the metabolism of testosterone, nandrolone and estradiol in cattle. Analyst 123: 2401-2404.
22. Singh, K. V., J. Kaur, G. C. Varshney, M. Raje, and C. R. Suri. 2004. Synthesis and characterization of hapten-protein conjugates for antibody production against small molecules. Bioconjug Chem. 15: 168-173.
23. Strahm, E., C. Saudan, P. E. Sottas, P. Mangin, and M. Saugy. 2007. Direct detection and quantification of 19-norandrosterone sulfate in human urine by liquid chromatographylinear ion trap mass spectrometry. J. Chromatogr. B 852: 491-496.
24. Watanabe, E., H. Eun, K. Baba, T. Arao, S. Endo, M. Ueji, and Y. Ishii. 2005. Synthesis of haptens for development of antibodies to alkylphenols and evaluation and optimization of a selected antibody for ELISA development. J. Agric. Food Chem. 53: 7395-7403.
25. Zhang, Y., S. Lu, W. Liu, C. Zhao, and R. Xi. 2007. Preparation of anti-Tetracycline antibodies and development of an indirect heterologous competitive enzyme-linked immunosorbent assay to detect residues of Tetracycline in milk. J. Agric. Food Chem. 55: 211-218.