Heterologous expression of a gene for thermostable xylanase from Chaetomium thermophilum in Pichia pastoris GS115

Molecular Biology Reports

Volumn 38, Issue 5, 2011, Pages 3227-3233

  Ghaffar, Abdul ^a   Khan, Sher Afzal ^b   Mukhtar, Zahid ^b   Rajoka, Muhammad Ibrahim ^b   Latif, Farooq ^b  

^a UNIVERSITY OF AGRICULTURE   (Pakistan)

^b NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING NIBGE   (Pakistan)

Author keywords

Extra cellular expression; Fungal xylanase; Gene cloning; Pichia pastoris; pPIC9K

Indexed keywords

XYLAN;

ARTICLE; CATALYSIS; CHAETOMIUM; CHAETOMIUM THERMOPHILUM; CONTROLLED STUDY; CULTURE OPTIMIZATION; ENTHALPY; ENTROPY; ENZYME ACTIVITY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS CULTURE; GENETIC STABILITY; HETEROLOGOUS EXPRESSION; INCUBATION TIME; MOLECULAR CLONING; NONHUMAN; PH; PICHIA PASTORIS; PROMOTER REGION; PROTEIN HYDROLYSIS; TEMPERATURE SENSITIVITY; THERMOSTABILITY; XYLANASE 11A GENE;

AMINO ACID SEQUENCE; CHAETOMIUM; CLONING, MOLECULAR; ENZYME STABILITY; FUNGAL PROTEINS; MOLECULAR SEQUENCE DATA; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; THERMODYNAMICS; XYLAN ENDO-1,3-BETA-XYLOSIDASE;

ANIMALIA; CHAETOMIUM THERMOPHILUM; PICHIA PASTORIS;

EID: 80052432240     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-010-9996-2     Document Type: Article

References (18)

1. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326-338 (Pubitemid 32763342)
2. Latif F, Asgher M, Saleem R, Akrem A, Legge RL (2006) Purification and characterization of a xylanase produced by Chaetomium thermophile NIBGE. World J Microbiol Biotechnol 22:347-348
3. de Faria FP, Te'O VSJ, Bergquist PL, Azevedo MO, Nevalainen KMH (2002) Excretion and processing of major xylanase (XYN2) from the thermophilic fungus Humicola grisea var.themoidea in Trichoderma reesei. Lett Appl Microbiol 34:119-123 (Pubitemid 34094029)
4. Cereghino G, Cereghino J, Ilgen C, Cregg J (2002) Production of recombinant proteins in fermentor cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13:329-332 (Pubitemid 35254090)
5. Damaso MCT, Almeida MS, Kurtenbach E, Martins OB, Pereira N Jr, Andrade CMMC, Albano RM (2003) Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris. Appl Environ Microbiol 69:6064-6072 (Pubitemid 37287580)
6. Tanaka H, Okuno T, Moriyama S, Muguruma M, Ohta K (2004) Acidophilic xylanase from Aureobasidium pullulans: efficient expression and secretion in Pichia pastoris and mutational analysis. J Bios Bioeng 98:338-343 (Pubitemid 40013198)
7. Bradford MM (1976) A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
8. Afzal AJ, Latif F, Rajoka MI, SiddiquiKS(2005) Innovative kinetic and thermodynamic studies of a purified superactive xylanase from Scopulariopsis sp. Appl Biochem Biotechnol 120:51-70 (Pubitemid 40279825)
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
10. Ruanglek V, Sriprang R, Ratanaphan N, Tirawongsaroj P, Chantasigh D, Tanapongpipat S, Pootanakit K, Eurwilaichitr L (2007) Cloning, expression, characterization, and high cell-density production of recombinant endo-1,4-b-xylanase from Aspergillus niger in Pichia pastoris. Enzyme Microb Technol 41:19-25 (Pubitemid 46729536)
11. Mäntylä A, Palheimo M, Hakola S, Lindberg E, Leskinen S, Kallio J, Vehmaaperä J, Lantto R, Souminen P (2007) Production in Trichoderma reesei of three xylanases from Chaetomium thermophile: a recombinant thermoxylanase for biobleaching. Appl Microbiol Biotechnol 76:377-386 (Pubitemid 47101684)
12. Christodoulou E, Vorgias CE (2002) The thermostability of the DNA-binding protein HU from mesophilic, thermophilic, and extreme thermophilic bacteria. Extremophile 61:21-31 (Pubitemid 41490489)
13. Henrissat B, Davies G (1997) Structural and sequence-based classification of glycosyl hydrolases. Curr Opin Struct Biol 7:637-644 (Pubitemid 27472582)
14. Chanta singh D, Pootanakit K, Champreda V, Kanokratana P, Eurwilaichitr L (2006) Cloning, expression, and characterization of a xylanase 10 from Aspergillus terreus (BCC129) in Pichia pastoris. Protein Expr Purif 46:143-149 (Pubitemid 43247007)
15. Zhang GM, Huang J, Huang GR, Ma LX, Zhang XE (2007) Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina. Appl Microbiol Biotechnol 74:339-346 (Pubitemid 46204509)
16. Rashid MH, Siddiqui KS (1998) Thermodynamic and kinetic study of stability of the native and chemically modified b-glucosidase from Aspergillus niger. Process Biochem 33:109-115 (Pubitemid 28128472)
17. Chen J, Lu Z, Sakkon J, Sites WE (2000) Increasing the thermostability of staphylococcal nuclease: implications for the origin of protein thermostability. J Mol Biol 303:125-130
18. Deng P, Li D, Cao Y, Lu W, Wang C (2006) Cloning of a gene encoding an acidophilic endo-b-1,4-xylanase obtained from Aspergillus niger CGMCC1067 and constitutive expression in Pichia pastoris. Enzyme Microb Technol 39:1096-1102 (Pubitemid 44062662)