Immobilised enzyme microreactor for screening of multi-step bioconversions: Characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols

Journal of Biotechnology

Volumn 155, Issue 3, 2011, Pages 320-329

  Matosevic, S ^a   Lye, G J ^a   Baganz, F ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Asymmetric synthesis; Enzyme immobilisation; Microfluidics; Microreactor; Transaminase; Transketolase

Indexed keywords

AMINO ALCOHOLS; ASYMMETRIC SYNTHESIS; CHARACTERISATION; CHIRAL AMINO ALCOHOLS; COMPLEX MOLECULES; CONTINUOUS FLOWS; CONTINUOUS-FLOW SYSTEM; GLYCOLALDEHYDES; IMMOBILISED ENZYMES; IMMOBILIZED ENZYME; IN-VITRO; LIVING CELL; MICROREACTOR; MICROREACTOR SYSTEM; MULTI-STEP; MULTISTEP REACTIONS; ONLINE MONITORING; STARTING COMPOUNDS; TRANSAMINASE; TRANSKETOLASE;

ALCOHOLS; ALDEHYDES; AMINATION; AMINES; BIOCONVERSION; CATALYSIS; KETONES; KINETIC PARAMETERS; LITHIUM; SYNTHESIS (CHEMICAL);

ENZYMES;

AMINE; AMINOALCOHOL; AMINOTRANSFERASE; GLYCOLALDEHYDE DEHYDROGENASE; HYDROXYPYRUVATE REDUCTASE; KETONE; LITHIUM; NICKEL; POLYHISTIDINE TAG; TRANSKETOLASE;

AMINATION; ARTICLE; BIOCATALYSIS; BIOTRANSFORMATION; CAPILLARY; CATALYSIS; CHEMICAL REACTION; CHIRALITY; CONTINUOUS FLOW REACTOR; CONTROLLED STUDY; IMMOBILIZED ENZYME REACTOR; PRIORITY JOURNAL; SYNTHESIS;

ACETALDEHYDE; ACETOPHENONES; AMINO ALCOHOLS; BIOREACTORS; ENZYMES, IMMOBILIZED; HISTIDINE; KINETICS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MODELS, BIOLOGICAL; OLIGOPEPTIDES; PYRUVATES; RECOMBINANT FUSION PROTEINS; STEREOISOMERISM; TETROSES; TRANSAMINASES; TRANSKETOLASE;

EID: 80052354312     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.07.017     Document Type: Article

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