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Volumn 76, Issue 7, 2004, Pages 1838-1843

Patterning Enzymes Inside Microfluidic Channels via Photoattachment Chemistry

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; GLASS; GLUCOSE; LIVING SYSTEMS STUDIES; MICROSCOPIC EXAMINATION;

EID: 1842581673     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac035234q     Document Type: Article
Times cited : (97)

References (22)
  • 14
    • 1842602780 scopus 로고    scopus 로고
    • note
    • In a separate experiment, polyelectrolytes were adsorbed in PDMS/glass microchannels and photopatterned with B4F. Poly(diallyldimethylammonium chloride), poly(styrenesulfonic acid) poly(acrylic acid), and polyethylenimine (the only polymer with primary amine substituents) were used. Only the last species gave rise to a visible pattern when labeled streptavidin was introduced. All surfaces suffered from nonspecific adsorption.
  • 16
    • 1842445997 scopus 로고    scopus 로고
    • note
    • Amplex Red can spontaneously oxidize to the fluorescent product, resorufin; however, we found that the substrate used for HRPLS had little if any background fluorescence when introduced into a microchannel with no enzyme. Therefore, we attribute all prepatch fluorescence to nonspecifically adsorbed enzyme.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.