Crystallographic analysis reveals a novel second binding site for trimethoprim in active site double mutants of human dihydrofolate reductase

Journal of Structural Biology

Volumn 176, Issue 1, 2011, Pages 52-59

  Cody, Vivian ^a,b   Pace, Jim ^a   Piraino, Jennifer ^a   Queener, Sherry F ^c  

^a HAUPTMAN WOODWARD MEDICAL RESEARCH INSTITUTE   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Crystal structure; Human dihydrofolate reductase; Kinetics; Mutagenesis; Pneumocystis jirovecii

Indexed keywords

DIHYDROFOLATE REDUCTASE; TRIMETHOPRIM;

ARTICLE; BINDING KINETICS; CRYSTAL STRUCTURE; DRUG BINDING SITE; DRUG TARGETING; ENZYME PURIFICATION; HUMAN; LIGAND BINDING; NONHUMAN; PNEUMOCYSTIS JIROVECI; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID MOTIFS; ANTIFUNGAL AGENTS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ENZYME ASSAYS; FUNGAL PROTEINS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; MUTATION, MISSENSE; PNEUMOCYSTIS JIROVECII; PROTEIN BINDING; SURFACE PROPERTIES; TETRAHYDROFOLATE DEHYDROGENASE; TRIMETHOPRIM;

MAMMALIA; PNEUMOCYSTIS; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS JIROVECII;

EID: 80052299234     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2011.06.001     Document Type: Article

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