Cloning, expression, and characterization of a new Xylanase from alkalophilic paenibacillus sp. 12-11

Journal of Microbiology and Biotechnology

Volumn 21, Issue 8, 2011, Pages 861-868

  Yanyu, Zhao ^a   Meng, Kun ^a   Luo, Huiying ^a   Yang, Peilong ^a   Shi, Pengjun ^a   Huang, Huoqing ^a   Bai, Yingguo ^a   Yao, Bin ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Alkaline xylanase; Escherichia coli; Paenibacillus sp.; Protease resistance

Indexed keywords

ARABINOXYLAN; BEECHWOOD XYLAN; BIRCHWOOD XYLAN; CARBOHYDRATE; OAT SPELT XYLAN; PROTEINASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; WHEAT ARABINOXYLAN; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOBIOSE; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE; GENE EXPRESSION; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PH; PROTEIN DEGRADATION; PROTEIN STABILITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; TEMPERATURE; THERMOSTABILITY; XYN7C GENE;

ALKALIES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; PAENIBACILLUS; SEQUENCE ALIGNMENT; SEWAGE; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; GOSSYPIUM HIRSUTUM; PAENIBACILLUS SP.; TRITICUM AESTIVUM;

EID: 80052290323     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1102.02024     Document Type: Article

References (40)

1. Bajpai, P. 1999. Application of enzymes in the pulp and paper industry. Biotechnol. Prog. 15: 147-157.
2. Beg, Q. K., M. Kapoor, L. Mahajan, and G. S. Hoondal. 2001. Microbial xylanases and their industrial applications: A review. Appl. Microbiol. Biotechnol. 56: 326-338.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Caballero, P. A., M. Gómez, and C. M. Rosell. 2007. Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination. J. Food Process Eng. 81: 42-53.
5. Christov, L. P., G. Szakacs, and H. Balakrishnan. 1999. Production, partial characterization and use of fungal cellulase-free xylanases in pulp bleaching. Process Biochem. 34: 511-517.
6. Collins, T., C. Gerday, and G. Feller. 2005. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 29: 3-23.
7. Gallardo, O., P. Diaz, and F. I. J. Pastor. 2003. Characterization of a Paenibacillus cell-associated xylanase with high activity on aryl-xylosides: A new subclass of family 10 xylanases. Appl. Microbiol. Biotechnol. 61: 226-233.
8. Gibbs, M. D., R. A. Reeves, and P. L. Bergquist. 1995. Cloning, sequencing, and expression of a xylanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B. 1 and activity of the enzyme on fiber-bound substrate. Appl. Environ. Microbiol. 61: 4403-4408.
9. Guo, B., X. Chen, C. Sun, B. Zhou, and Y. Zhang. 2009. Gene cloning, expression and characterization of a new cold-active and salt-tolerant endo-β-1,4-xylanase from marine Glaciecola mesophila KMM 241. Appl. Microbiol. Biotechnol. 84: 1107-1115.
10. Henrissat, B. and A. Bairoch. 1996. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316: 695-696.
11. Huang, J., G. Wang, and L. Xiao. 2006. Cloning, sequencing and expression of the xylanase gene from a Bacillus subtilis strain B10 in Escherichia coli. Bioresour. Technol. 97: 802-808.
12. Hwang, I. T., H. K. Lim, H. Y. Song, S. J. Cho, J. S. Chang, and N. J. Park. 2010. Cloning and characterization of a xylanase, KRICT PX1 from the strain Paenibacillus sp. HPL-001. Biotechnol. Adv. 28: 594-601.
13. Kim, D. Y., M. K. Han, H. W. Oh, K. S. Bae, T. S. Jeong, S. U. Kim, et al. 2010. Novel intracellular GH10 xylanase from Cohnella laeviribosi HY-21: Biocatalytic properties and alterations of substrate specificities by site-directed mutagenesis of Trp residues. Bioresour. Technol. 101: 8814-8821.
14. Ko, C. H., W. L. Chen, C. H. Tsai, W. N. Jane, C. C. Liu, and J. Tu. 2007. Paenibacillus campinasensis BL11: A wood materialutilizing bacterial strain isolated from black liquor. Bioresour. Technol. 98: 2727-2733.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
16. Lee, H., D. Shin, N. Cho, H. Kim, S. Shin, S. Im, H. Blaise Lee, S. Chun, and S. Bai. 2000. Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp. Biotechnol. Lett. 22: 387-392.
17. Li, N., K. Meng, Y. Wang, P. Shi, H. Luo, Y. Bai, P. Yang, and B. Yao. 2008. Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9. Appl. Microbiol. Biotechnol. 80: 231-240.
18. Lineweaver, H. and D. Burk. 1934. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56: 658-666.
19. Liu, L., X. Li, and W. Shao. 2004 Computational analysis of responsible dipeptides for optimum pH in G/11 xylanase. Biochem. Biophys. Res. Commun. 321: 391-396.
20. Liu, Y. and R. F. Whittier. 1995. Thermal asymmetric interlaced PCR: Automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25: 674-681.
21. Maat, J., M. Roza, J. Verbakel, H. Stam, M. J. Santos Da Silva, M. Bosse, et al. 1992. Xylanases and their application in bakery, pp. 349-360. In J. Visser, M. A. Kusters van Someren, G. Beldman, and A. G. J. Voragen (eds.). Xylans and Xylanases. Progress in Biotechnology, No. 7. Elsevier Science Publishers, Amsterdam, The Netherlands.
22. Mamo, G., R. Hatti-Kaul, and B. Mattiasson. 2006. A thermostable alkaline active endo-β-1-4-xylanase from Bacillus halodurans S7: Purification and characterization. Enzyme Microb. Technol. 39: 1492-1498.
23. Mamo, G., M. Thunnissen, R. Hatti-Kaul, and B. Mattiasson. 2009. An alkaline active xylanase: Insights into mechanisms of high pH catalytic adaptation. Biochimie 91: 1187-1196.
24. Manikandan, K., A. Bhardwaj, N. Gupta, N. K. Lokanath, A. Ghosh, V. S. Reddy, and S. Ramakumar. 2006. Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: Structural insights into alkalophilicity and implications for adaptation to polyextreme conditions. Protein Sci. 15: 1951-1960.
25. Mchunu, N. P., S. Singh, and K. Permaul. 2009. Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli. J. Biotechnol. 141: 26-30.
26. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
27. Monis, P. T., S. Giglio, and C. P. Saint. 2005. Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal. Biochem. 340: 24-34.
28. Ossola, M. and Y. M. Galante. 2004. Scouring of flax rove with the aid of enzymes. Enzyme Microb. Technol. 34: 177-186.
29. Qiu, Z., P. Shi, H. Luo, Y. Bai, T. Yuan, P. Yang, S. Liu, and B. Yao. 2010. A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry. Enzyme Microb. Technol. 46: 506-512.
30. Roberge, M., F. Shareck, R. Morosoli, D. Kluepfel, and C. Dupont. 1998. Site-directed mutagenesis study of a conserved residue in family 10 glycanases: Histidine 86 of xylanase A from Streptomyces lividans. Protein Eng. 11: 399-404.
31. Schmidt, A., A. Schlacher, W. Steiner, H. Schwab, and C. Kratky. 1998. Structure of the xylanase from Penicillium simplicissimum. Protein Sci. 7: 2081-2088.
32. Subramaniyan, S. and P. Prema. 2000. Cellulase-free xylanases from Bacillus and other microorganisms. FEMS Microbiol. Lett. 183: 1-7.
33. Subramaniyan, S. and P. Prema. 2002. Biotechnology of microbial xylanases: Enzymology, molecular biology, and application. Crit. Rev. Biotechnol. 22: 33-64.
34. Sudo, M., M. Sakka, T. Kimyra, K. Ratanakhanokchai, and K. Sakka. 2010. Characterization of Paenibacillus curdlanolyticus intracellular xylanase Xyn10B encoded by the xyn10B gene. Biosci. Biotechnol. Biochem. 74: 2358-2360.
35. Sun, H. J., S. Yoshida, Y. Kawabata, N. H. Park, and I. Kusakabe. 2002. Separation of two functional domains of the family F/10 β-xylanase from Streptomyces olivaceoviridis E-86 limited proteolysis with papain and some of their properties. Biotechnol. Lett. 24: 595-601.
36. Wang, G., Y. Wang, P. Yang, H. Luo, H. Huang, P. Shi, K. Meng, and B. Yao. 2010. Molecular detection and diversity of xylanase genes in alpine tundra soil. Appl. Microbiol. Biotechnol. 87: 1383-1393.
37. Wang, J., Y. Bai, P. Yang, P. Shi, H. Luo, K. Meng, H. Huang, J. Yin, and B. Yao. 2010. A new xylanase from thermoalkaline Anoxybacillus sp. E2 with high activity and stability over a broad pH range. World J. Microbiol. Biotechnol. 26: 917-924.
38. Wang, Q., X. Fan, W. Gao, and J. Chen. 2006. Scouring of knitted cotton fabrics with compound enzymes. J. Text. Res. 27: 27-30.
39. Wood, P. J., J. D. Erfle, and R. M. Teather. 1988. Use of complex formation between Congo red and polysaccharides in detection and assay of polysaccharide hydrolases. Methods Enzymol. 160: 59-74.
40. Yu, E. K. C., L. U. L. Tan, M. K. H. Chan, L. Deschatelets, and J. N. Saddler. 1987. Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus. Enzyme Microb. Technol. 9: 16-24.