Emulsion culture: A miniaturized library screening system based on micro-droplets in an emulsified medium

Journal of Bioscience and Bioengineering

Volumn 112, Issue 3, 2011, Pages 299-303

  Kojima, Takaaki ^a   Nagao, Nobuhito ^a   Ando, Daisuke ^a   Ojima, Teruyo ^a   Kawarasaki, Yasuaki ^b   Kobayashi, Isao ^c   Nakajima, Mitsutoshi ^d   Nakano, Hideo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

^c NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^d UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Emulsion culture; Micro droplets; Microchannel; Saccharomyces cerevisiae; Signal peptide library screening

Indexed keywords

AGAR PLATES; CULTURE MEDIUM; CULTURE METHODS; DIRECTED EVOLUTION; EMULSION-CULTURE; ENRICHMENT CULTURE; ENRICHMENT FACTORS; EXTRACELLULAR; HIGH-DENSITY; LIBRARY SCREENING; MICRO DROPLETS; MICROCOLONIES; NOVEL METHODS; PHYSICAL SEPARATION; POTENTIAL VARIATIONS; SIGNAL PEPTIDE;

CLONING; DROPS; ENZYMES; PEPTIDES; YEAST;

EMULSIFICATION;

BETA GALACTOSIDASE; ENZYME VARIANT;

ARTICLE; CARBON SOURCE; CONTROLLED STUDY; CULTURE MEDIUM; EMULSION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; FUNGAL CELL CULTURE; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; NONHUMAN; PROTEIN EXPRESSION; YEAST; YEAST CELL;

AMINO ACID SEQUENCE; BETA-GALACTOSIDASE; CLINICAL LABORATORY TECHNIQUES; EMULSIONS; HUMANS; PEPTIDE LIBRARY; PROTEIN SORTING SIGNALS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 80052259926     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.05.017     Document Type: Article

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