Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions

Journal of Biological Chemistry

Volumn 286, Issue 35, 2011, Pages 30878-30887

  Tomlinson, Christopher G ^a   Syson, Karl ^a,d   Sengerová, Blanka ^a,e   Atack, John M ^a   Sayers, Jon R ^a   Swanson, Linda ^a   Tainer, John A ^b,c   Williams, Nicholas H ^a   Grasby, Jane A ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d JOHN INNES CENTRE   (United Kingdom)

^e UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ISOTHERMS; BIPHASIC; CALCIUM IONS; CHEMICAL CATALYSIS; COFACTORS; COMBINED ANALYSIS; CONCENTRATION RANGES; DIESTERS; DISSOCIATION CONSTANT; DIVALENT METALS; ENDONUCLEASES; ENZYME-SUBSTRATE COMPLEXES; MAXIMAL RATES; METAL COFACTORS; OVERALL REACTIONS; PHOSPHODIESTERASES; SUBSTRATE BINDING; WILD TYPES;

BINDING SITES; CALCIUM; CARBOXYLATION; CATALYSIS; CHEMICAL ANALYSIS; DISSOCIATION; ENZYMES; METAL IONS; WETLANDS;

METALS;

CALCIUM ION; CARBOXYLIC ACID DERIVATIVE; ENDONUCLEASE; MAGNESIUM ION; METAL; T5 FLAP ENDONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; COMPLEX FORMATION; CONCENTRATION RESPONSE; DISSOCIATION CONSTANT; ENZYME STABILITY; ISOTHERM; PRIORITY JOURNAL;

BINDING SITES; BIOPHYSICS; CALCIUM; CATALYSIS; CATALYTIC DOMAIN; DNA; ENZYMES; EXODEOXYRIBONUCLEASES; IONS; KINETICS; MAGNESIUM; METALS; MOLECULAR CONFORMATION; MUTATION; PHOSPHATES; PROTEIN BINDING; SOFTWARE;

EID: 80052258482     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.230391     Document Type: Article

References (41)

1. Tomlinson, C. G., Atack, J. M., Chapados, B., Tainer, J. A., and Grasby, J. A. (2010) Biochem. Soc. Trans. 38, 433-437
2. Orans, J., McSweeney, E. A., Iyer, R. R., Hast, M. A., Hellinga, H. W., Modrich, P., and Beese, L. S. (2011) Cell 145, 212-223
3. Syson, K., Tomlinson, C., Chapados, B. R., Sayers, J. R., Tainer, J. A., Williams, N. H., and Grasby, J. A. (2008) J. Biol. Chem. 283, 28741-28746
4. Tsutakawa, S. E., Classen, S., Chapados, B. R., Arvai, A. S., Finger, L. D., Guenther, G., Tomlinson, C. G., Thompson, P., Sarker, A. H., Shen, B., Cooper, P. K., Grasby, J. A., and Tainer, J. A. (2011) Cell 145, 198-211
5. Yang, W. (2011) Q. Rev. Biophys. 44, 1-93
6. Allen, L. M., Hodskinson, M. R., and Sayers, J. R. (2009) Biochem. J. 418, 285-292
7. Sigel, R. K., and Pyle, A. M. (2007) Chem. Rev. 107, 97-113
8. Yang, W., Lee, J. Y., and Nowotny, M. (2006) Mol. Cell 22, 5-13
9. Pingoud, A., Fuxreiter, M., Pingoud, V., and Wende, W. (2005) Cell. Mol. Life Sci. 62, 685-707
10. Dupureur, C. M. (2010) Metallomics 2, 609-620
11. Zheng, L., Li, M., Shan, J., Krishnamoorthi, R., and Shen, B. (2002) Biochemistry 41, 10323-10331
12. Feng, M., Patel, D., Dervan, J. J., Ceska, T., Suck, D., Haq, I., and Sayers, J. R. (2004) Nat. Struct. Mol. Biol. 11, 450-456
13. Hosfield, D. J., Mol, C. D., Shen, B., and Tainer, J. A. (1998) Cell 95, 135-146
14. Mueser, T. C., Nossal, N. G., and Hyde, C. C. (1996) Cell 85, 1101-1112
15. Devos, J. M., Tomanicek, S. J., Jones, C. E., Nossal, N. G., and Mueser, T. C. (2007) J. Biol. Chem. 282, 31713-31724
16. Tock, M. R., Frary, E., Sayers, J. R., and Grasby, J. A. (2003) EMBO J. 22, 995-1004
17. Ceska, T. A., Sayers, J. R., Stier, G., and Suck, D. (1996) Nature 382, 90-93
18. Sakurai, S., Kitano, K., Yamaguchi, H., Hamada, K., Okada, K., Fukuda, K., Uchida, M., Ohtsuka, E., Morioka, H., and Hakoshima, T. (2005) EMBO J. 24, 683-693
19. Hwang, K. Y., Baek, K., Kim, H. Y., and Cho, Y. (1998) Nature Struct. Biol. 5, 707-713
20. Chapados, B. R., Hosfield, D. J., Han, S., Qiu, J., Yelent, B., Shen, B., and Tainer, J. A. (2004) Cell 116, 39-50
21. Bhagwat, M., Meara, D., and Nossal, N. G. (1997) J. Biol. Chem. 272, 28531-28538
22. Kim, Y., Eom, S. H., Wang, J., Lee, D. S., Suh, S. W., and Steitz, T. A. (1995) Nature 376, 612-616
23. Gangisetty, O., Jones, C. E., Bhagwat, M., and Nossal, N. G. (2005) J. Biol. Chem. 280, 12876-12887
24. Sengerová, B., Tomlinson, C., Atack, J. M., Williams, R., Sayers, J. R., Williams, N. H., and Grasby, J. A. (2010) Biochemistry 49, 8085-8093
25. Williams, R., Sengerová, B., Osborne, S., Syson, K., Ault, S., Kilgour, A., Chapados, B. R., Tainer, J. A., Sayers, J. R., and Grasby, J. A. (2007) J. Mol. Biol. 371, 34-48
26. Patel, D., Tock, M. R., Frary, E., Feng, M., Pickering, T. J., Grasby, J. A., and Sayers, J. R. (2002) J. Mol. Biol. 320, 1025-1035
27. Reid, S. L., Parry, D., Liu, H. H., and Connolly, B. A. (2001) Biochemistry 40, 2484-2494
28. Lagunavicius, A., Grazulis, S., Balciunaite, E., Vainius, D., and Siksnys, V. (1997) Biochemistry 36, 11093-11099
29. Dervan, J. J., Feng, M., Patel, D., Grasby, J. A., Artymiuk, P. J., Ceska, T. A., and Sayers, J. R. (2002) Proc. Natl. Acad. Sci. 99, 8542-8547
30. Pickering, T. J., Garforth, S., Sayers, J. R., and Grasby, J. A. (1999) J. Biol. Chem. 274, 17711-17717
31. Datta, K., and LiCata, V. J. (2003) J. Biol. Chem. 278, 5694-5701
32. Bastock, J. A., Webb, M., and Grasby, J. A. (2007) J. Mol. Biol. 368, 421-433
33. Pickering, T. J., Garforth, S. J., Thorpe, S. J., Sayers, J. R., and Grasby, J. A. (1999) Nucleic Acids Res. 27, 730-735
34. Xu, Y., Derbyshire, V., Ng, K., Sun, X. C., Grindley, N. D., and Joyce, C. M. (1997) J. Mol. Biol. 268, 284-302
35. Hohl, M., Thorel, F., Clarkson, S. G., and Schärer, O. D. (2003) J. Biol. Chem. 278, 19500-19508
36. Rass, U., Compton, S. A., Matos, J., Singleton, M. R., Ip, S. C., Blanco, M. G., Griffith, J. D., and West, S. C. (2010) Genes Dev. 24, 1559-1569
37. Finger, L. D., Blanchard, M. S., Theimer, C. A., Sengerová, B., Singh, P., Chavez, V., Liu, F., Grasby, J. A., and Shen, B. (2009) J. Biol. Chem. 284, 22184-22194
38. Lee, B. I., and Wilson, D. M., 3rd (1999) J. Biol. Chem. 274, 37763-37769
39. Chang, J. H., Xiang, S., Xiang, K., Manley, J. L., and Tong, L. (2011) Nat. Struct. Mol. Biol. 18, 270-276
40. Jinek, M., Coyle, S. M., and Doudna, J. A. (2011) Mol. Cell 41, 600-608
41. Xiang, S., Cooper-Morgan, A., Jiao, X., Kiledjian, M., Manley, J. L., and Tong, L. (2009) Nature 458, 784-788