A single cleavage assay for T5 5'→3' exonuclease: Determination of the catalytic parameters for wild-type and mutant proteins

Nucleic Acids Research

Volumn 27, Issue 3, 1999, Pages 730-735

  Pickering, Timothy J ^a   Garforth, Scott J ^a,b   Thorpe, Simon J ^a   Sayers, Jon R ^a   Grasby, Jane A ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; LYSINE; MUTANT PROTEIN; PHOSPHODIESTERASE I;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE T5; CATALYSIS; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ASSAY; ENZYME DEGRADATION; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; BASE SEQUENCE; CATALYSIS; EXODEOXYRIBONUCLEASES; KINETICS; LYSINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POINT MUTATION; PROTEINS; T-PHAGES;

EID: 0033080780     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/27.3.730     Document Type: Article

References (26)

1. Lieber, M.R. (1997) Bioessay, 19, 233-240.
2. Harrington, J.J. and Lieber, M.R. (1994) EMBO J., 13, 1235-1246.
3. Garforth, S.J. and Sayers, J.R. (1997) Nucleic Acids Res., 25, 3801-3807.
4. Lyamichev, V., Brow, M.A.D. and Dahlberg, J.E. (1993) Science, 260, 778-783.
5. Ceska, J.A., Sayers, J.R., Stier, G. and Suck, D. (1996) Nature, 382, 90-93.
6. Kim, Y., Eom, S.H., Wang, J.M., Lee, D.S., Suh, S.W. and Steitz, T.A. (1995) Nature, 376, 612-616.
7. Hwang, K.Y., Baek, K., Kim, H.Y. and Cho, Y. (1998) Nature Struct. Biol., 5, 707-713.
8. Mueser, T.C., Nossal, N.C. and Hyde, C.C. (1996) Cell, 85, 1101-1112.
9. Shen, B.H., Nolan, J.P., Sklar, L.A. and Park, M.S. (1996) J. Biol. Chem., 271, 9173-9176.
10. Garforth, S., Ceska, T., Suck, D. and Sayers, J. (1999) Proc. Natl Acad. Sci. USA, in press.
11. Fraser, M. (1980) Methods Enzymol., 65, 255-263.
12. Nolan, J.P., Shen, B.H., Park, M.S. and Sklar, L.A. (1996) Biochemistry, 35, 11668-11676.
13. Shen, B.H., Nolan, J.P., Sklar, LA. and Park, M.S. (1997) Nucleic Acids Res., 25, 3332-3338.
14. Seela, F. and Kehne, A. (1985) Biochemistry, 24, 7556-7561.
15. Bradford, M. (1976) Anal. Biochem., 72, 248-254.
16. Xu, Y., Derbyshire, V., Ng, K., Sun, X.C., Grindley, N.D.F. and Joyce, C.M. (1997) J. Mol. Biol., 268, 284-302.
17. Sayers, J.R. and Eckstein, F. (1990) J. Biol. Chem., 265, 18311-18317.
18. Young, K.J., Gill, F. and Grasby, J.A. (1997) Nucleic Acids Res., 25, 3760-3766.
19. Grasby, J.A., Butler, P.J.G. and Gait, M.J. (1993) Nucleic Acids Res., 21, 4444-4450.
20. Wolfenden, R., Ridgway, C. and Young, G. (1998) J. Am. Chem. Soc., 120, 833-834.
21. Kim, J.H. and Chin, J. (1992) J. Am. Chem. Soc., 114, 9792-9795.
22. Ceska, T.A. and Sayers J.R. (1998) Trends Biochem. Sci., 23, 331-336.
23. Fersht, A.R. (1988) Biochemistry, 27, 1577-1580.
24. Gerlt, JA and Gassman, P.G. (1993) Biochemistry, 32, 11943-11952.
25. Messmore, J.M., Fuchs, D.N. and Raines, R.T. (1995) J. Am. Chem. Soc., 117, 8057-8060.
26. Selent, U., Ruter, T., Kohler, E., Liedtke, M., Thielking, V., Alves, J., Oelgeschlager, T., Wolfes, H., Peters, F. and Pingoud, A. (1992) Biochemistry, 31, 4808-4815.