Determination of blood leukocyte concentration with constant volume acquisition on a flow cytometer is comparable to individualized single platform testing with beads as internal reference standard

Journal of Immunological Methods

Volumn 338, Issue 1-2, 2008, Pages 58-62

  Hansen, Susan ^a   Dahl, Ronald ^a   Hoffmann, Hans Jürgen ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

CD45 PanLeukoGating; Flow cytometry; Single platform testing

Indexed keywords

ALLERGEN;

ADULT; ALGORITHM; ARTICLE; CELL ACTIVATION; CONSTANT VOLUME ACQUISITION; CONTROLLED STUDY; FLOW CYTOMETRY; FLOW RATE; HUMAN; HUMAN CELL; IMMUNOTHERAPY; LEUKOCYTE; LEUKOCYTE COUNT; MICROSCOPY; NORMAL HUMAN; PARAMETER; PREDICTOR VARIABLE; PRIORITY JOURNAL; SINGLE PLATFORM TECHNOLOGY; STANDARD; TECHNOLOGY;

ADULT; FLOW CYTOMETRY; HUMANS; LEUKOCYTE COUNT; MICROSCOPY; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS;

EID: 50949114723     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.07.010     Document Type: Article

References (11)

1. Bergeron M., Lustyik G., Phaneuf S., Ding T., Nicholson J.K.A., Janossy G., Shapiro H., Barnett D., and Mandy F. Stability of currently used cytometers facilitates the identification of pipetting errors and their volumetric operation: "time" can tell all. Cytometry B Clin. Cytom. 52 (2003) 37
2. Bousquet J., Müller U.R., Dreborg S., Jarisch R., Malling H.J., Mosbech H., Urbanek R., and Youlten L. Immunotherapy with Hymenoptera venoms. Position paper of the Working Group on Immunotherapy of the European Academy of Allergy and Clinical Immunology. Allergy 42 (1987) 401
3. Glencross D., Scott L.E., Jani I.V., Barnett D., and Janossy G. CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration. Cytometry 50 (2002) 69
4. + T cells. Int. Arch. Allergy Immunol. 140 (2006) 252
5. Hoffmann H.J., Malling T.M., Topcu A., Ryder L.P., Nielsen K.R., Varming K., Dahl R., Omland O., and Sigsgaard T. CD4(dim)CD25(bright) Treg cell frequencies above a standardized gating threshold are similar in asthmatics and controls. Cytometry A 71 (2007) 371
6. Martini F., D'Offizi G., Girardi E., Vitale A., Cimini E., Mollicone B., Vlassi C., and Aiuti F. The 2005 Italian Quality Control Study for the evaluation of CD4 cells in centers involved in the treatment of HIV type 1 patients. AIDS Res. Hum. Retroviruses 23 (2007) 777
7. Nicholson J.K., Stein D., Mui T., Mack R., Hubbard M., and Denny T. Evaluation of a method for counting absolute numbers of cells with a flow cytometer. Clin. Diagn. Lab. Immunol. 4 (1997) 309
8. Schnizlein-Bick C.T., Spritzler J., Wilkening C.L., Nicholson J.K., and O'Gorman M.R. Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group. Clin. Diagn. Lab. Immunol. 7 (2000) 336
9. Storie I., Sawle A., Goodfellow K., Whitby L., Granger V., Reilly J.T., and Barnett D. Flow rate calibration I: a novel approach for performing absolute cell counts. Cytometry B Clin. Cytom. 55 (2003) 1
10. Walker C.L., Whitby L., Granger V., Storie I., Reilly J.T., and Barnett D. Flow rate calibration. III. The use of stabilized biostandards to calibrate the flow rate and calculate absolute CD4+ T-cell counts. Cytometry B Clin. Cytom. 70 (2006) 154
11. Yarchoan R., Venzon D.J., Pluda J.M., Lietzau J., Wyvill K.M., Tsiatis A.A., Steinberg S.M., and Broder S. CD4 count and the risk for death in patients infected with HIV receiving antiretroviral therapy. Ann. Intern. Med. 115 (1991) 184