Stable and high-level production of recombinant Factor IX in human hepatic cell line

Biotechnology and Applied Biochemistry

Volumn 58, Issue 4, 2011, Pages 243-249

  De Castilho Fernandes, Andrielle ^a,b   Fontes, Aparecida ^a,b   Gonsales, Nathalia ^a,b   Swiech, Kamilla ^b   Picanco Castro, Virginia ^b   Faca, Sandra ^b   Covas, Dimas ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b National Institute of Science and Technology in Stem Cell Therapy   (Brazil)

Author keywords

293 and HepG2 cell lines; hemophilia B; recombinant human blood coagulation Factor IX; retroviral vector mediated transfer

Indexed keywords

293 CELLS; ACTIVATED PARTIAL THROMBOPLASTIN TIME; ACTIVE PROTEINS; B-MODELS; CELL LINES; COAGULATION SYSTEM; GENETIC DISEASE; HEMOPHILIA B; HEPATIC CELLS; HEPG2 CELL LINE; HIGH-LEVEL PRODUCTION; HUMAN EMBRYONIC KIDNEYS; HUMAN PLASMAS; IN-VITRO; PHARMACOKINETIC PROFILES; RECOMBINANT CELLS; RECOMBINANT HUMAN BLOOD COAGULATION FACTOR IX; RECOMBINANT HUMAN FACTOR IX; RETROVIRAL VECTOR-MEDIATED TRANSFER; REVERSED-PHASE CHROMATOGRAPHY;

CELL CULTURE; COAGULATION; DEVELOPING COUNTRIES; HUMAN ENGINEERING;

CELLS;

ANTIBIOTIC G 418; BLOOD CLOTTING FACTOR 9; OCTANINE; RECOMBINANT BLOOD CLOTTING FACTOR 9; RETROVIRUS VECTOR; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CELL STRAIN HEK293; CELL STRAIN HEPG2; CONTROLLED STUDY; COST CONTROL; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENETIC TRANSDUCTION; HEMOPHILIA B; HUMAN; HUMAN CELL; LIVER CELL; MOUSE; NONHUMAN; PARTIAL THROMBOPLASTIN TIME; REAL TIME POLYMERASE CHAIN REACTION; REVERSED PHASE LIQUID CHROMATOGRAPHY; WESTERN BLOTTING;

ANIMALS; BIOTECHNOLOGY; CHROMATOGRAPHY, REVERSE-PHASE; FACTOR IX; GENE EXPRESSION; GENETIC VECTORS; HEK293 CELLS; HEMOPHILIA B; HEP G2 CELLS; HUMANS; MICE; PARTIAL THROMBOPLASTIN TIME; RECOMBINANT PROTEINS; RETROVIRIDAE; TRANSDUCTION, GENETIC;

EID: 80051776297     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.32     Document Type: Article

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