The carboxylation efficiency of the vitamin K-dependent clotting factors: Studies with factor IX

Haemophilia

Volumn 14, Issue 5, 2008, Pages 1063-1068

  Blostein, Mark ^a   Cuerquis, J ^a   Landry, S ^a   Galipeau, J ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Chromatography; Factor IX; Gamma carboxylation; Gene therapy; Haemophilia; Hydroxyapatite

Indexed keywords

BLOOD CLOTTING FACTOR 9; COMPLEMENTARY DNA; HYDROXYAPATITE; PROTHROMBIN; RECOMBINANT BLOOD CLOTTING FACTOR 9; VITAMIN K GROUP;

ARTICLE; CARBOXYLATION; CELL STRAIN HEK293; CHROMATOGRAPHY; CONTROLLED STUDY; DRUG SYNTHESIS; GENE EXPRESSION SYSTEM; GENETIC TRANSFECTION; HEMOPHILIA B; HUMAN; HUMAN CELL; IN VITRO STUDY; NONVIRAL GENE THERAPY; PRIORITY JOURNAL;

BLOTTING, WESTERN; CARBON-CARBON LIGASES; CELL LINE; CHROMATOGRAPHY, LIQUID; DNA, COMPLEMENTARY; FACTOR IX; HUMANS; PROTHROMBIN; RECOMBINANT PROTEINS; TRANSFECTION; VITAMIN K;

EID: 51249114084     PISSN: 13518216     EISSN: 13652516     Source Type: Journal    
DOI: 10.1111/j.1365-2516.2008.01828.x     Document Type: Article

References (19)

1. Mannucci PM, Tuddenham EG. The hemophilias - from royal genes to gene therapy. N Engl J Med 2001; 344: 1773-9.
2. Furie B, Furie BC. Molecular basis of vitamin K-dependent gamma-carboxylation. Blood 1990; 75: 1753-62.
3. Harrison S, Adamson S, Bonam D et al. The manufacturing process for recombinant factor IX. Semin Hematol 1998; 35: 4-10.
4. Furie B, Bouchard BA, Furie BC. Vitamin K-dependent biosynthesis of gamma-carboxyglutamic acid. Blood 1999; 93: 1798-808.
5. Stafford DW. The vitamin K cycle. J Thromb Haemost 2005; 3: 1873-8.
6. Camire RM, Larson PJ, Stafford DW et al. Enhanced gamma-carboxylation of recombinant factor X using a chimeric construct containing the prothrombin propeptide. Biochemistry 2000; 39: 14322-9.
7. Horton RM, Hunt HD, Ho SN et al. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension. Gene 1989; 77: 61-8.
8. Stanley TB, Jin DY, Lin PJ et al. The propeptides of the vitamin K-dependent proteins possess different affinities for the vitamin K-dependent carboxylase. J Biol Chem 1999; 274: 16940-4.
9. Paquin A, Jaalouk DE, Galipeau J. Retrovector encoding a green fluorescent protein-herpes simplex virus thymidine kinase fusion protein serves as a versatile suicide/reporter for cell and gene therapy applications. Hum Gene Ther 2001; 12: 13-23.
10. Bristol JA, Ratcliffe JV, Roth DA et al. Biosynthesis of prothrombin: intracellular localization of the vitamin K-dependent carboxylase and the sites of gamma-carboxylation. Blood 1996; 88: 2585-93.
11. Rehemtulla A, Roth DA, Wasley LC et al. In vitro and in vivo functional characterization of bovine vitamin K-dependent gamma-carboxylase expressed in Chinese hamster ovary cells. Proc Natl Acad Sci USA 1993; 90: 4611-5.
12. Larson PJ, Camire RM, Wong D et al. Structure/function analyses of recombinant variants of human factor Xa: Factor Xa incorporation into prothrombinase on the thrombin-activated platelet surface is not mimicked by synthetic phospholipid vesicles. Biochemistry 1998; 37: 5029-38.
13. Sun YM, Jin DY, Camire RM et al. Vitamin K epoxide reductase significantly improves carboxylation in a cell line overexpressing factor X. Blood 2005; 106: 3811-5.
14. Berkner KL. Expression of recombinant vitamin K-dependent proteins in mammalian cells: Factors IX and VII. Methods Enzymol 1993; 222: 450-77.
15. Wajih N, Hutson SM, Owen J et al. Increased production of functional recombinant human clotting factor IX by baby hamster kidney cells engineered to overexpress VKORC1, the vitamin K 2,3-epoxide-reducing enzyme of the vitamin K cycle. J Biol Chem 2005; 280: 31603-7.
16. Rost S, Fregin A, Ivaskevicius V et al. Mutations in VKORC1 cause warfarin resistance and multiple coagulation factor deficiency type 2. Nature 2004; 427: 537-41.
17. Rieder MJ, Reiner AP, Gage BF et al. Effect of VKORC1 haplotypes on transcriptional regulation and warfarin dose. N Engl J Med 2005; 352: 2285-93.
18. Wadelius M, Chen LY, Downes K et al. Common VKORC1 and GGCX polymorphisms associated with warfarin dose. Pharmacogenomics J 2005; 5: 262-70.
19. Yuan HY, Chen JJ, Lee MT et al. A novel functional VKORC1 promoter polymorphism is associated with inter-individual and inter-ethnic differences in warfarin sensitivity. Hum Mol Genet 2005; 14: 1745-51.