Multiplex real-time PCR for detection of respiratory tract infections

Journal of Clinical Virology

Volumn 41, Issue 1, 2008, Pages 53-56

  Brittain Long, Robin ^a   Nord, Sandra ^a   Olofsson, Sigvard ^a   Westin, Johan ^a   Anderson, Lars Magnus ^a   Lindh, Magnus ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Molecular diagnostics; Real time PCR; Respiratory infection; Taqman

Indexed keywords

ADENOVIRUS; ARTICLE; CORONAVIRUS; ENTEROVIRUS; HUMAN; HUMAN METAPNEUMOVIRUS; HUMAN TISSUE; INFLUENZA VIRUS A; INFLUENZA VIRUS B; MYCOPLASMA PNEUMONIAE; NONHUMAN; NOSE SMEAR; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 3; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY TRACT INFECTION; RHINOVIRUS; THROAT CULTURE;

HUMANS; PHARYNX; POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS; VIRUS DISEASES; VIRUSES;

EID: 37549035421     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2007.10.029     Document Type: Article

References (14)

1. Bellau-Pujol S., Vabret A., Legrand L., Dina J., Gouarin S., Petitjean-Lecherbonnier J., et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 126 (2005) 53-63
2. Creer D.D., Dilworth J.P., Gillespie S.H., Johnston A.R., Johnston S.L., Ling C., et al. Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care. Thorax 61 (2006) 75-79
3. Diaz A., Barria P., Niederman M., Restrepo M.I., Dreyse J., Fuentes G., et al. Etiology of community-acquired pneumonia in hospitalized patients in Chile: the increasing prevalence of respiratory viruses among classic pathogens. Chest 131 (2007) 779-787
4. Gruteke P., Glas A.S., Dierdorp M., Vreede W.B., Pilon J.W., and Bruisten S.M. Practical implementation of a multiplex PCR for acute respiratory tract infections in children. J Clin Microbiol 42 (2004) 5596-5603
5. Gunson R.N., Collins T.C., and Carman W.F. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J Clin Virol 33 (2005) 341-344
6. Heim A., Ebnet C., Harste G., and Pring-Akerblom P. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 70 (2003) 228-239
7. Kuypers J., Wright N., Ferrenberg J., Huang M.L., Cent A., Corey L., et al. Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. J Clin Microbiol 44 (2006) 2382-2388
8. Li H., McCormac M.A., Estes R.W., Sefers S.E., Dare R.K., Chappell J.D., et al. Simultaneous detection and high-throughput identification of a panel of RNA viruses causing respiratory tract infections. J Clin Microbiol 45 (2007) 2105-2109
9. Liolios L., Jenney A., Spelman D., Kotsimbos T., Catton M., and Wesselingh S. Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J Clin Microbiol 39 (2001) 2779-2783
10. Lundborg C.S., Olsson E., and Molstad S. Antibiotic prescribing in outpatients: a 1-week diagnosis-prescribing study in 5 counties in Sweden. Scand J Infect Dis 34 (2002) 442-448
11. Puppe W., Weigl J.A., Aron G., Grondahl B., Schmitt H.J., Niesters H.G., et al. Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens. J Clin Virol 30 (2004) 165-174
12. Templeton K.E., Scheltinga S.A., Beersma M.F., Kroes A.C., and Claas E.C. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol 42 (2004) 1564-1569
13. Ward C.L., Dempsey M.H., Ring C.J., Kempson R.E., Zhang L., Gor D., et al. Design and performance testing of quantitative real-time PCR assays for influenza A and B viral load measurement. J Clin Virol 29 (2004) 179-188
14. Watzinger F., Suda M., Preuner S., Baumgartinger R., Ebner K., Baskova L., et al. Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J Clin Microbiol 42 (2004) 5189-5198