Cysteine scanning mutagenesis (residues Glu 52-Gly 96) of the human P2X1 receptor for ATP: Mapping agonist binding and channel gating

Journal of Biological Chemistry

Volumn 286, Issue 33, 2011, Pages 29207-29217

  Allsopp, Rebecca C ^a   El Ajouz, Sam ^a   Schmid, Ralf ^a   Evans, Richard J ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AGONIST BINDING SITE; ATP BINDING POCKETS; BINDING POCKETS; CATION CHANNELS; CHANNEL GATING; CYSTEINE-SCANNING MUTAGENESIS; EXTRACELLULAR; EXTRACELLULAR DOMAINS; IN-SILICO; IONIC PERMEATION; METHANETHIOSULFONATE; MOLECULAR BASIS; MOLECULAR DOCKING; P2X RECEPTORS; SURAMIN; TRANSMEMBRANE REGIONS; X-RAY STRUCTURE;

CHEMICAL REACTIONS; MUTAGENESIS;

AMINO ACIDS;

3' O (4 BENZOYL)BENZOYL ADENOSINE 5' TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; GLUTAMIC ACID; GLYCINE; METHANETHIOSULFONATE; MUTANT PROTEIN; PURINERGIC P2X1 RECEPTOR; PYRIDOXAL PHOSPHATE 6 AZOPHENYL 2',4' DISULFONIC ACID; SULFONIC ACID DERIVATIVE; SURAMIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CHANNEL GATING; CONTROLLED STUDY; CYSTEINE SCANNING MUTAGENESIS; HUMAN; ION PERMEABILITY; MOLECULAR DOCKING; NONHUMAN; OOCYTE; PRIORITY JOURNAL; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS; XENOPUS LAEVIS;

ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; HUMANS; ION CHANNEL GATING; MODELS, MOLECULAR; MUTAGENESIS; MUTATION, MISSENSE; PEPTIDE MAPPING; RECEPTORS, PURINERGIC P2X1; XENOPUS LAEVIS;

EID: 80051696222     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.260364     Document Type: Article

References (37)

1. North, R. A. (2002) Physiol. Rev. 82, 1013-1067
2. Burnstock, G. (2006) Pharmacol. Rev. 58, 58-86
3. Khakh, B. S., and North, R. A. (2006) Nature 442, 527-532
4. Surprenant, A., and North, R. A. (2009) Annu. Rev. Physiol. 71, 333-359
5. Roberts, J. A., Vial, C., Digby, H. R., Agboh, K. C., Wen, H., Atterbury-Thomas, A., and Evans, R. J. (2006) Pflugers Arch. 452, 486-500
6. Roberts, J. A., Valente, M., Allsopp, R. C., Watt, D., and Evans, R. J. (2009) J. Neurochem. 109, 1042-1052
7. Kawate, T., Michel, J. C., Birdsong, W. T., and Gouaux, E. (2009) Nature 460, 592-598
8. Garcia-Guzman, M., Soto, F., Gomez-Hernandez, J. M., Lund, P. E., and Stühmer, W. (1997) Mol. Pharmacol. 51, 109-118
9. Roberts, J. A., and Evans, R. J. (2007) J. Neurosci. 27, 4072-4082
10. Ennion, S., Hagan, S., and Evans, R. J. (2000) J. Biol. Chem. 275, 29361-29367
11. Martí-Renom, M. A., Stuart, A. C., Fiser, A., Sánchez, R., Melo, F., and Sali, A. (2000) Annu. Rev. Biophys. Biomol. Struct. 29, 291-325
12. Hooft, R. W., Vriend, G., Sander, C., and Abola, E. E. (1996) Nature 381, 272
13. Wiederstein, M., and Sippl, M. J. (2007) Nucleic Acids Res. 35, W407-410
14. Verdonk, M. L., Cole, J. C., Hartshorn, M. J., Murray, C. W., and Taylor, R. D. (2003) Proteins 52, 609-623
15. Roberts, J. A., Digby, H. R., Kara, M., El Ajouz, S., Sutcliffe, M. J., and Evans, R. J. (2008) J. Biol. Chem. 283, 20126-20136
16. Ho, B. K., and Gruswitz, F. (2008) BMC Struct. Biol. 8, 49
17. Digby, H. R., Roberts, J. A., Sutcliffe, M. J., and Evans, R. J. (2005) J. Neurochem. 95, 1746-1754
18. Jiang, L. H., Rassendren, F., Surprenant, A., and North, R. A. (2000) J. Biol. Chem. 275, 34190-34196
19. Jiang, L. H., Rassendren, F., Spelta, V., Surprenant, A., and North, R. A. (2001) J. Biol. Chem. 276, 14902-14908
20. Roberts, J. A., and Evans, R. J. (2004) J. Biol. Chem. 279, 9043-9055
21. Bodnar, M., Wang, H., Riedel, T., Hintze, S., Kato, E., Fallah, G., Gröger-Arndt, H., Giniatullin, R., Grohmann, M., Hausmann, R., Schmalzing, G., Illes, P., and Rubini, P. (2011) J. Biol. Chem. 286, 2739-2749
22. Wolf, C., Rosefort, C., Fallah, G., Kassack, M. U., Hamacher, A., Bodnar, M., Wang, H., Illes, P., Kless, A., Bahrenberg, G., Schmalzing, G., and Hausmann, R. (2011) Mol. Pharmacol. 79, 649-661
23. Evans, R. J., Lewis, C., Buell, G., Valera, S., North, R. A., and Surprenant, A. (1995) Mol. Pharmacol. 48, 178-183
24. Dunn, P. M., and Blakeley, A. G. (1988) Br. J. Pharmacol. 93, 243-245
25. Lambrecht, G., Friebe, T., Grimm, U., Windscheif, U., Bungardt, E., Hildebrandt, C., Bäumert, H. G., Spatz-Kümbel, G., and Mutschler, E. (1992) Eur. J. Pharmacol. 217, 217-219
26. Akabas, M. H., Stauffer, D. A., Xu, M., and Karlin, A. (1992) Science 258, 307-310
27. Rassendren, F., Buell, G., Newbolt, A., North, R. A., and Surprenant, A. (1997) EMBO J. 16, 3446-3454
28. Evans, R. J. (2010) Br. J. Pharmacol. 161, 961-971
29. Zemkova, H., Yan, Z., Liang, Z., Jelinkova, I., Tomic, M., and Stojilkovic, S. S. (2007) J. Neurochem. 102, 1139-1150
30. Ralevic, V., Hoyle, C. H., and Burnstock, G. (1995) J. Physiol. 483, 703-713
31. Adriouch, S., Bannas, P., Schwarz, N., Fliegert, R., Guse, A. H., Seman, M., Haag, F., and Koch-Nolte, F. (2008) FASEB J. 22, 861-869
32. Marquez-Klaka, B., Rettinger, J., Bhargava, Y., Eisele, T., and Nicke, A. (2007) J. Neurosci. 27, 1456-1466
33. Marquez-Klaka, B., Rettinger, J., and Nicke, A. (2009) Eur. Biophys. J. 38, 329-338
34. Jin, R., Banke, T. G., Mayer, M. L., Traynelis, S. F., and Gouaux, E. (2003) Nat. Neurosci. 6, 803-810
35. Lape, R., Colquhoun, D., and Sivilotti, L. G. (2008) Nature 454, 722-727
36. Kawate, T., Robertson, J. L., Li, M., Silberberg, S. D., and Swartz, K. J. (2011) J. Gen. Physiol. 137, 579-590
37. Jiang, R., Lemoine, D., Martz, A., Taly, A., Gonin, S., Prado de Carvalho, L., Specht, A., and Grutter, T. (2011) Proc. Natl. Acad. Sci. USA 108, 9066-9071