Improvement of ethanol production in Saccharomyces cerevisiae by hetero-expression of GAPN and FPS1 deletion

Journal of Chemical Technology and Biotechnology

Volumn 86, Issue 9, 2011, Pages 1205-1210

  Wang, Pin Mei ^a   Zheng, Dao Qiong ^a   Ding, Rui ^a   Chi, Xiao Qin ^a   Tao, Xiang Lin ^a   Min, Hang ^a   Wu, Xue Chang ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Ethanol; FPS1; Glyceraldehydes 3 phosphate dehydrogenase; Glycerol; Saccharomyces cerevisiae; Streptococcus mutans

Indexed keywords

ANAEROBIC FERMENTATION; CARBON FLUXES; COMBINATION STRATEGIES; ETHANOL PRODUCTION; FPS1; GLYCERALDEHYDES-3-PHOSPHATE DEHYDROGENASE; PARENTAL STRAINS; REDOX BALANCES; S.CEREVISIAE; STREPTOCOCCUS MUTANS;

ACETIC ACID; BYPRODUCTS; ETHANOL; FERMENTATION; GLYCEROL; GROWTH KINETICS;

YEAST;

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCEROL; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PROTEIN FPS1; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SACCHAROMYCES CEREVISIAE PROTEIN; UNCLASSIFIED DRUG;

ALCOHOL PRODUCTION; ANAEROBIC FERMENTATION; ARTICLE; BACTERIAL GROWTH; FUNGAL STRAIN; GENE DELETION; GENE EXPRESSION; GROWTH RATE; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE; STREPTOCOCCUS MUTANS;

EID: 80051668575     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.2634     Document Type: Article

References (21)

1. Arshad M, Khan ZM, Khalil ur R, Shah FA and Rajoka MI, Optimization of process variables for minimization of byproduct formation during fermentation of blackstrap molasses to ethanol at industrial scale. Lett Appl Microbiol 47: 410-414 (2008).
2. Medina VG, Almering MJ, van Maris AJ and Pronk JT, Elimination of glycerol production in anaerobic cultures of a Saccharomyces cerevisiae strain engineered to use acetic acid as an electron acceptor. Appl Environ Microbiol 76: 190-195 (2010).
3. Bjorkqvist S, Ansell R, Adler L and Liden G, Physiological response to anaerobicity of glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae. Appl Environ Microbiol 63: 128-132 (1997).
4. Hohmann S, Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol Rev 66: 300-372 (2002).
5. Remize F, Barnavon L and Dequin S, Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. Metab Eng 3: 301-312 (2001).
6. Nissen T, Kielland-Brandt M, Nielsen J and Villadsen J, Optimization of ethanol production in Saccharomyces cerevisiae by metabolic engineering of the ammonium assimilation. Metab Eng 2: 69-77 (2000).
7. Guo Z, Zhang L, Ding Z, Wang Z and Shi G, Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Appl Microbiol Biotechnol 82: 287-292 (2009).
8. Zhang A, Kong Q, Cao L and Chen X, Effect of FPS1 deletion on the fermentation properties of Saccharomyces cerevisiae. Lett Appl Microbiol 44: 212-217 (2007).
9. Kong Q, Gu J, Cao L, Zhang A, Chen X and Zhao X, Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae. Biotechnol Lett 28: 2033-2038 (2006).
10. Cao L, Zhang A, Kong Q, Xu X, Josine T and Chen X, Overexpression of GLT1 in fps1ΔgpdΔ mutant for optimum ethanol formation by Saccharomyces cerevisiae. Biomol Eng 24: 638-642 (2007).
11. Kong QX, Cao LM, Zhang AL and Chen X, Overexpressing GLT1 in gpd1Delta mutant to improve the production of ethanol of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 73: 1382-1386 (2007).
12. Kong QX, Zhang AL, Cao LM, and Chen X, Over-expressing GLT1 in a gpd2Delta mutant of Saccharomyces cerevisiae to improve ethanol production. Appl Microbiol Biotechnol 75: 1361-1366 (2007).
13. Bro C, Regenberg B, Forster J and Nielsen J, In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production. Metab Eng 8: 102-111 (2006).
14. Boyd DA, Cvitkovitch DG, and Hamilton IR, Sequence, expression, and function of the gene for the nonphosphorylating, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Streptococcus mutans. J Bacteriol 177: 2622-2627 (1995).
15. +-dependent glyceraldehyde-3-phosphate dehydrogenases from Streptococcus mutans. J Biol Chem 254: 1134-1142 (1979).
16. Wojda I, Alonso-Monge R, Bebelman JP, Mager WH and Siderius M, Response to high osmotic conditions and elevated temperature in Saccharomyces cerevisiae is controlled by intracellular glycerol and involves coordinate activity of MAP kinase pathways. Microbiology 149: 1193-1204 (2003).
17. Erten H and Tanguler H, Influence of Williopsis saturnus yeasts in combination with Saccharomyces cerevisiae on wine fermentation. Lett Appl Microbiol 50: 474-479 (2010).
18. Valadi H, Valadi A, Ansell R, Gustafsson L, Adler L, Norbeck J et al, NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1). Curr Genet 45: 90-95 (2004).
19. Tamás MJ, Luyten K, Sutherland FC, Hernandez A, Albertyn J, Valadi H, et al, Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation. Mol Microbiol 31: 1087-1104 (1999).
20. Bill RM, Hedfalk K, Karlgren S, Mullins JG, Rydstrom J and Hohmann S, Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1p. J Biol Chem 276: 36543-36549 (2001).
21. Nissen TL, Schulze U, Nielsen J and Villadsen J, Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae. Microbiology 143: 203-218 (1997).