Toll-like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide

Journal of Urology

Volumn 186, Issue 3, 2011, Pages 1084-1092

  Smith, Nicholas J ^a,b   Varley, Claire L ^a   Eardley, Ian ^b   Feather, Sally ^b   Trejdosiewicz, Ludwik K ^a   Southgate, Jennifer ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b ST JAMES S UNIVERSITY HOSPITAL   (United Kingdom)

Author keywords

toll like receptors; urinary bladder; urinary tract infections; uropathogenic Escherichia coli; urothelium

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; CD14 ANTIGEN; FLAGELLIN; I KAPPA B; INTERLEUKIN 6; INTERLEUKIN 8; TOLL LIKE RECEPTOR; TOLL LIKE RECEPTOR 1; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 3; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 5; TOLL LIKE RECEPTOR 6; TOLL LIKE RECEPTOR 8;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOKINE RELEASE; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KIDNEY CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; TRANSITIONAL CELL CARCINOMA; UROTHELIUM;

CELLS, CULTURED; EPITHELIAL CELLS; ESCHERICHIA COLI; FLAGELLIN; HUMANS; LIPOPOLYSACCHARIDES; TOLL-LIKE RECEPTOR 4; TOLL-LIKE RECEPTOR 5; UROTHELIUM;

EID: 80051551361     PISSN: 00225347     EISSN: 15273792     Source Type: Journal    
DOI: 10.1016/j.juro.2011.04.112     Document Type: Article

References (29)

1. A.T. Gewirtz, T.A. Navas, and S. Lyons Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression J Immunol 167 2001 1882
2. R. Medzhitov, P. Preston-Hurlburt, and C.A. Janeway Jr A human homologue of the Drosophila Toll protein signals activation of adaptive immunity Nature 388 1997 394
3. R. Shimazu, S. Akashi, and H. Ogata MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4 J Exp Med 189 1999 1777
4. S.D. Wright, R.A. Ramos, and P.S. Tobias CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein Science 249 1990 1431
5. H. Fischer, M. Yamamoto, and S. Akira Mechanism of pathogen-specific TLR4 activation in the mucosa: fimbriae, recognition receptors and adaptor protein selection Eur J Immunol 36 2006 267
6. A. Poltorak, X. He, and I. Smirnova Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene Science 282 1998 2085
7. J.D. Schilling, S.M. Martin, and C.S. Hung Toll-like receptor 4 on stromal and hematopoietic cells mediates innate resistance to uropathogenic Escherichia coli Proc Natl Acad Sci USA 100 2003 4203
8. P. Samuelsson, L. Hang, and B. Wullt Toll-like receptor 4 expression and cytokine responses in the human urinary tract mucosa Infect Immunol 72 2004 3179
9. S. Hedges, P. Anderson, and G. Lidin-Janson Interleukin-6 response to deliberate colonization of the human urinary tract with gram-negative bacteria Infect Immunol 59 1991 421
10. Y.C. Ko, N. Mukaida, and S. Ishiyama Elevated interleukin-8 levels in the urine of patients with urinary tract infections Infect Immunol 61 1993 1307
11. B. Ragnarsdottir, K. Jonsson, and A. Urbano Toll-like receptor 4 promoter polymorphisms: common TLR4 variants may protect against severe urinary tract infection PLoS One 5 2010 e10734
12. J.D. Schilling, S.M. Martin, and D.A. Hunstad CD14- and Toll-like receptor-dependent activation of bladder epithelial cells by lipopolysaccharide and type 1 piliated Escherichia coli Infect Immunol 71 2003 1470
13. J. Song, M.J. Duncan, and G. Li A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells PLoS Pathog 3 2007 e60
14. F. Backhed, M. Soderhall, and P. Ekman Induction of innate immune responses by Escherichia coli and purified lipopolysaccharide correlate with organ- and cell-specific expression of Toll-like receptors within the human urinary tract Cell Microbiol 3 2001 153
15. F. Backhed, L. Meijer, and S. Normark TLR4-dependent recognition of lipopolysaccharide by epithelial cells requires sCD14 Cell Microbiol 4 2002 493
16. E. Andersen-Nissen, T.R. Hawn, and K.D. Smith Cutting edge: Tlr5-/- mice are more susceptible to Escherichia coli urinary tract infection J Immunol 178 2007 4717
17. W.R. Cross, I. Eardley, and H.J. Leese A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function Am J Physiol Renal Physiol 289 2005 F459
18. P.C. Rubenwolf, N.T. Georgopoulos, and L.A. Clements Expression and localisation of aquaporin water channels in human urothelium in situ and in vitro Eur Urol 56 2009 1013
19. J. Southgate, K.A. Hutton, and D.F. Thomas Normal human urothelial cells in vitro: proliferation and induction of stratification Lab Invest 71 1994 583
20. J. Southgate, J.R. Masters, and L.K. Trejdosiewicz Culture of human urothelium R.I. Freshney, M.G. Freshney, Culture of Epithelial Cells 2nd ed. 2002 J. Wiley and Sons New York 381 400
21. J. Southgate, C.L. Varley, and M.A. Garthwaite Differentiation potential of urothelium from patients with benign bladder dysfunction BJU Int 99 2007 1506
22. J. Southgate, and L.K. Trejdosiewicz Immunolabelling of cells and tissues: approaches and pitfalls Hum Reprod 12 suppl 1997 65
23. C.L. Varley, J. Stahlschmidt, and W.C. Lee Role of PPARgamma and EGFR signalling in the urothelial terminal differentiation programme J Cell Sci 117 2004 2029
24. A.A. Baqui, T.F. Meiller, and W.A. Falkler Enhanced interleukin-8 production in THP-1 human monocytic cells by lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor Oral Microbiol Immunol 14 1999 275
25. S. Takashiba, T.E. Van Dyke, and S. Amar Differentiation of monocytes to macrophages primes cells for lipopolysaccharide stimulation via accumulation of cytoplasmic nuclear factor kappaB Infect Immunol 67 1999 5573
26. C.U. Duerr, S.F. Zenk, and C. Chassin O-antigen delays lipopolysaccharide recognition and impairs antibacterial host defense in murine intestinal epithelial cells PLoS Pathog 5 2009 e1000567
27. M.W. Hornef, T. Frisan, and A. Vandewalle Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells J Exp Med 195 2002 559
28. C. Cirl, A. Wieser, and M. Yadav Subversion of Toll-like receptor signaling by a unique family of bacterial Toll/interleukin-1 receptor domain-containing proteins Nat Med 14 2008 399
29. F.J. Sheedy, E. Palsson-McDermott, and E.J. Hennessy Negative regulation of TLR4 via targeting of the proinflammatory tumor suppressor PDCD4 by the microRNA miR-21 Nat Immunol 11 2010 141