Immunolabelling of cells and tissues: Approaches and pitfalls

Human Reproduction

Volumn 12, Issue SUPPL., 1997, Pages 65-75

  Southgate, J ^a   Trejdosiewicz, L K ^a  

^a ST JAMES S UNIVERSITY HOSPITAL   (United Kingdom)

Author keywords

Antibody; Immunochemistry; Immunofluorescence; Phenotype; Technique

Indexed keywords

EID: 0001916344     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Avrameas, S. and Ternynck, T. (1967) Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J. Biol. Chem., 242, 1651.
2. Bancroft, J.D. and Stevens, A. (1990) In Theory and Practice of Histological Techniques. 3rd edn. Churchill Livingstone, Edinburgh.
3. Baricordi, O.R., Sensi, A., De Vinci, C. et al. (1985) A monoclonal antibody to human transitional-cell carcinoma of the bladder cross-reacting with a differentiation antigen of neutrophilic lineage. Int. J. Cancer, 35, 781-786.
4. Brandtzaeg, P. and Baklien, K. (1976) Inconclusive immunohistochemistry of human IgE in mucosal pathology. Lancet, i, 1297-1298.
5. Catty, D., Kennedy, J.F., Drew, R.L. and Tun, H.C. (1973) The application of cellulose carbonate to the preparation of water-insoluble immunoadsorbents used in the purification of antibodies to immunoglobulins. J. Immunol. Methods. 2, 353-369.
6. Chan, J.K., Tsang, W.Y. and Pau, M.Y. (1995) Discordant CD3 expression in lymphomas when studied on frozen and paraffin sections. Hum. Pathol., 26, 1139-1143.
7. Cordell, J.L., Falini, B., Erber, W.N. et al. (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem., 32, 219-229.
8. De May, J. Hacker, G.W., Dewaele, M. and Springall, D.R. (1986) Gold probes in light microscopy. In Polak, J.M. and Van Noorden, S. (eds), Immunocytochemistry, Modern Methods and Applications. 2nd edn. Wright, Bristol, pp. 71-89.
9. + phenotypes are increased in coeliac disease. Scand. J. Immunol., 30, 665-672.
10. Harlow, E. and Lane, D. (1988) Antibodies, A Laboratory Manual. Cold Spring Harbor Laboratory, New York.
11. Harnden, P. and Southgate, J. (1996) Oestrogen receptor status of mast cells in interstitial cystitis. Br. J. Urol., 77, 474-475.
12. Hodges, G.M., Toulson, B.C. and Southgate, J. (1987) Colloidal gold-a powerful tool in scanning electron microscope immunocytochemistry: an overview of bioapplications. Scanning Microsc., 1, 301-318.
13. Hsu, S.-M., Raine, L. and Fanger, H. (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem., 29, 577-580.
14. Joniau, M., Onkelinx, E. and Lontie, R. (1968) Glutaraldehyde polymerized proteins as immunosorbents. Arch. Int. Physiol. Biochim., 76, 182-183.
15. Lane, D. and Koprowski, H. (1982) Molecular recognition and the future of monoclonal antibodies. Nature, 296, 200-202.
16. Lane, D.P. and Lane, E.B. (1981). A rapid antibody assay system for screening hybridoma cultures. J. Immunol. Meth., 47, 303-307.
17. Lane, E.B., Goodman, S.L., Trejdosiewicz, L.K. (1982) Disruption of the keratin filament network during epithelial cell division. EMBO J., 1, 1365-1372.
18. Lan, H.Y., Mu, W., Nikolic-Paterson, D.J. and Atkins, R.C. (1995) A novel, simple, reliable, and sensitive method for multiple immunoenzyme staining: use of microwave oven heating to block antibody crossreactivity and retrieve antigens. J. Histochem. Cytochem., 43, 97-102.
19. Metzgar, R.S., Borowitz, M.J., Jones, N.H. and Dowell, B.L. (1981) Distribution of common acute lymphoblastic leukemia antigen in nonhematopoietic tissues. J. Exp. Med., 154, 1249-1254.
20. Polak, J.M., and Van Noorden, S. (eds) (1986) Immunocytochemistry, Modern Methods and Applications. 2nd edn. Wright, Bristol.
21. Schenk, E.A. and Churukian, C.J. (1974) Immunofluorescence counterstains. J. Histochem. Cytochem., 22, 962-966.
22. Schüller-Petrovic, S., Gebhart, W., Lassmann, H. et al. (1983) A shared antigenic determinant between natural killer cells and nervous tissue. Nature, 306, 179-181.
23. Southgate, J., Harnden, P. and Holt, J. (1996) Which proliferation markers for routine immunohistology? J. Clin. Pathol., 49, 270.
24. Trejdosiewicz, L.K., Smolira, M.A., Hodges, G.M. et al. (1981) Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence. J. Microscopy, 123, 227-236.
25. Trejdosiewicz, L.K., Wilson, P.D. and Hodges, G.M. (1984) Species cross-reactive membrane-associated urothelial differentiation antigen. J. Natl. Cancer Inst., 72, 355-366.
26. Trejdosiewicz, L.K., Southgate, J., Hodges, G.M. and Goodman, S.L. (1985) Micro-heterogenous expression of peanut agglutinin-binding sites in the extracellular matrix of cultured cells. Exp. Cell. Res., 156, 153-163.
27. Trejdosiewicz, L.K., Smart, C.J., Oakes, D.J. et al. (1989) Expression of T-cell receptors TcR1 (γ/δ) and TcR2 (α/β) in the human intestinal mucosa. Immunology, 68, 7-12.