Heterologous pyc gene expression under various natural and engineered promoters in Escherichia coli for improved succinate production

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 236-243

  Thakker, Chandresh ^a   Zhu, Jiangfeng ^b,c   San, Ka Yiu ^b   Bennett, George ^a  

^a RICE UNIVERSITY   (United States)

^b Rice University   (United States)

^c QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

Author keywords

Escherichia coli; Fine tuning gene expression; Promoter engineering; Pyruvate carboxylase; Succinate

Indexed keywords

COPY NUMBER; E. COLI; EXPRESSION LEVELS; FINE TUNING; FINE-TUNING GENE EXPRESSION; LACTOCOCCUS LACTIS; PROMOTER ENGINEERING; PYRUVATE CARBOXYLASE; SITE DIRECTED MUTAGENESIS; SUCCINATE; SUCCINATE PRODUCTION;

DNA; ESCHERICHIA COLI;

GENE EXPRESSION;

DNA POLYMERASE; ENDONUCLEASE; PYRUVATE CARBOXYLASE; SUCCINIC ACID;

ARTICLE; BACTERIAL STRAIN; BINDING SITE; CELL DENSITY; COPY NUMBER VARIATION; CYTOLYSIS; DNA EXTRACTION; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; LACTOCOCCUS LACTIS; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN STABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BIOTECHNOLOGY; ESCHERICHIA COLI; GENE TRANSFER TECHNIQUES; GENES, BACTERIAL; GENETIC ENGINEERING; LACTOCOCCUS LACTIS; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROMOTER REGIONS, GENETIC; SUCCINIC ACID;

ESCHERICHIA COLI; LACTOCOCCUS LACTIS;

EID: 80051469649     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.05.001     Document Type: Article

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