Mytilus galloprovincialis myticin C: A chemotactic molecule with antiviral activity and immunoregulatory properties

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Balseiro, Pablo ^a   Falcó, Alberto ^b   Romero, Alejandro ^a   Dios, Sonia ^a   Martínez López, Alicia ^b   Figueras, Antonio ^a   Estepa, Amparo ^b   Novoa, Beatriz ^a  

^a INSTITUTO DE INVESTIGACIONES MARINAS   (Spain)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIVIRUS AGENT; CELL EXTRACT; COMPLEMENT COMPONENT C1Q; COMPLEMENT COMPONENT MGC1Q; COMPLEMENTARY DNA; LYSOZYME; MYTICIN B; MYTICIN C; MYTILIN; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; ANTIMICROBIAL CATIONIC PEPTIDE; CHEMOTACTIC FACTOR; GREEN FLUORESCENT PROTEIN; IMMUNOLOGIC FACTOR; ISOPROTEIN; MESSENGER RNA; MYTICIN; PEPTIDE; PLASMA PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ANTIVIRAL ACTIVITY; ARTICLE; BLOOD CELL; CELL LINE; CONTROLLED STUDY; EMBRYO; FISH; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC VARIABILITY; IMMUNE SYSTEM; IMMUNOREGULATION; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; MUSSEL; MYTILUS GALLOPROVINCIALIS; NONHUMAN; NUCLEOTIDE SEQUENCE; ANIMAL; CELL MOTION; FLUORESCENCE MICROSCOPY; GENE EXPRESSION PROFILING; GENETIC TRANSFECTION; GENETICS; GILL; GROWTH, DEVELOPMENT AND AGING; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; METABOLISM; MOLECULAR GENETICS; MYTILUS; RABBIT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

BIVALVIA; INVERTEBRATA; MYTILUS GALLOPROVINCIALIS;

ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; ANTIVIRAL AGENTS; BLOOD PROTEINS; CELL LINE; CELL MOVEMENT; CHEMOTACTIC FACTORS; GENE EXPRESSION PROFILING; GILLS; GREEN FLUORESCENT PROTEINS; HEMOCYTES; IMMUNOHISTOCHEMISTRY; IMMUNOLOGIC FACTORS; IN SITU HYBRIDIZATION; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; MYTILUS; NOVIRHABDOVIRUS; PEPTIDES; PROTEIN ISOFORMS; RABBITS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSFECTION;

EID: 79961197723     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023140     Document Type: Article

References (56)

1. Falcó A, Brocal I, Pérez L, Coll JM, Estepa A, et al. (2008) In vivo modulation of the rainbow trout (Oncorhynchus mykiss) immune response by the human alpha defensin 1, HNP1. Fish Shellfish Immunol 24: 102-112.
2. Patrzykat A, Douglas SE, (2005) Antimicrobial peptides: Cooperative approaches to protection. Protein Peptide Lett 12: 19-25.
3. Zasloff M, (2002) Antimicrobial peptides of multicellular organisms. Nature 415: 389-395.
4. Oppenheim JJ, Biragyn A, Kwak LW, Yang D, (2003) Roles of antimicrobial peptides such as defensins in innate and adaptive immunity. Ann Rheum Dis 62 (suppl 2): ii17-ii21.
5. Klotman ME, Chang TL, (2006) Defensins in innate antiviral immunity. Nat Rev Immunol 6: 447-456.
6. Ulvatne H, Haukland HH, Samuelsen Ø, Krämer M, Vorland LH, (2002) Proteases in Escherichia coli and Staphylococcus aureus confer reduced susceptibility to lactoferricin B. J Antimicrob Chemother 50: 461-467.
7. Brown KL, Hancock RE, (2006) Cationic host defense (antimicrobial) peptides. Curr Opin Immunol 18: 24-30.
8. Mitta G, Hubert F, Dyrynda EA, Boudry P, Roch P, (2000) Mytilin B and MGD2, two antimicrobial peptides of marine mussels: gene structure and expression analysis. Dev Comp Immunol 24: 381-393.
9. Mitta G, Hubert F, Noel T, Roch P, (1999) Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. Eur J Biochem 265: 71-78.
10. Roch P, Yang Y, Toubiana M, Aumelas A, (2008) NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides. Dev Comp Immunol 32: 227-238.
11. Costa MM, Dios S, Alonso-Gutiérrez J, Romero A, Novoa B, et al. (2009) Evidence of high individual diversity on myticin C in mussel (Mytilus galloprovincialis). Dev Comp Immunol 33: 162-170.
12. Pallavicini A, Costa MM, Gestal C, Dreos R, Figueras A, et al. (2008) High sequence variability of myticin transcripts in hemocytes of immune-stimulated mussels suggests ancient host-pathogen interactions. Dev Comp Immunol 32: 213-226.
13. Padhi A, Verghese B, (2008) Molecular diversity and evolution of myticin-C antimicrobial peptide variants in the Mediterranean mussel, Mytilus galloprovincialis. Peptides 29: 1094-1101.
14. Battison AL, (2008) Isolation and characterisation of two antimicrobial peptides from haemocytes of the American lobster Homarus americanus. Fish Shellfish Immunol 25: 181-187.
15. Mitta G, Vandenbulcke F, Noel T, Romestand B, Beauvillain JC, et al. (2000) Differential distribution and defence involvement of antimicrobial peptides in mussel. J Cell Sci 113 (Pt 15): 2759-2769.
16. Mitta G, Vandenbulcke F, Hubert F, Salzet M, Roch P, (2000) Involvement of mytilins in mussel antimicrobial defense. J Biol Chem 275: 12954-12962.
17. Govorin I, (2000) Role of bivalves in the depuration of seawaters contaminated by bacteria. Russ J Mar Biol 26: 81-88.
18. Birkbeck TH, McHenery JG, (1982) Degradation of bacteria by Mytilus edulis. Mar Biol 72: 7-15.
19. Mitta G, Vandenbulcke F, Hubert F, Roch P, (1999) Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci 112 (Pt 23): 4233-4242.
20. Hancock REW, Sahl H-G, (2006) Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol 24: 1551-1557.
21. Ding J, Chou YY, Chang TL, (2009) Defensins in viral infections. J Innate Immun 1: 413-420.
22. Falcó A, Mas V, Tafalla C, Pérez L, Coll JM, et al. (2007) Dual antiviral activity of human alpha-defensin-1 against viral haemorrhagic septicaemia rhabdovirus (VHSV): Inactivation of virus particles and induction of a type I interferon-related response. Antiviral Res 76: 111-123.
23. Falcó A, Ortega-Villaizan M, Chico V, Brocal I, Perez L, et al. (2009) Antimicrobial peptides as model molecules for the development of novel antiviral agents in aquaculture. Mini Rev Med Chem 9: 1159-1164.
24. Roch P, Beschin A, Bernard E, (2004) Antiprotozoan and antiviral activities of non-cytotoxic truncated and variant analogues of mussel defensin. Evid Based Complement Alternat Med 1: 167-174.
25. Li H, Venier P, Prado-Álvarez M, Gestal C, Toubiana M, et al. (2010) Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection. Fish Shellfish Immunol 28: 640-648.
26. Mitta G, Vandenbulcke F, Roch P, (2000) Original involvement of antimicrobial peptides in mussel innate immunity. FEBS Lett 486: 185-190.
27. Gestal C, Pallavicini A, Venier P, Novoa B, Figueras A, (2010) MgC1q, a novel C1q-domain-containing protein involved in the immune response of Mytilus galloprovincialis. Dev Comp Immunol 34: 926-934.
28. Kong P, Zhang H, Wang L, Zhou Z, Yang J, et al. (2010) AiC1qDC-1, a novel gC1q-domain-containing protein from bay scallop Argopecten irradians with fungi agglutinating activity. Dev Comp Immunol 34: 837-846.
29. Zhang H, Song L, Li C, Zhao J, Wang H, et al. (2008) A novel C1q-domain-containing protein from Zhikong scallop Chlamys farreri with lipopolysaccharide binding activity. Fish Shellfish Immunol 25: 281-289.
30. Chu FL, La Peyre JF, (1989) Effect of environmental factors and parasitism on hemolymph lysozyme and protein of American oysters (Crassostrea virginica). J Invertebr Pathol 54: 224-232.
31. McDade JE, Tripp MR, (1967) Lysozyme in the hemolymph of the oyster, Crassostrea virginica. J Invertebr Pathol 9: 531-535.
32. Jollès P, Jollès J, (1984) What's new in lysozyme research? Mol Cell Biochem 63: 165-189.
33. Hong XT, Xiang LX, Shao JZ, (2006) The immunostimulating effect of bacterial genomic DNA on the innate immune responses of bivalve mussel, Hyriopsis cumingii Lea. Fish Shellfish Immunol 21: 357-364.
34. Chen Y, Xiang LX, Shao JZ, (2007) Construction of a recombinant plasmid containing multi-copy CpG motifs and its effects on the innate immune responses of aquatic animals. Fish Shellfish Immunol 23: 589-600.
35. Mogensen TH, (2009) Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev 22: 240-273.
36. Kumazawa NH, Shimoji Y, (1991) Plasma-dependent chemotactic activity of hemocytes derived from a juvenile estuarine gastropod mollusc, Clithon retropictus, to Vibrio parahaemolyticus and Escherichia coli strains. J Vet Med Sci 53: 883-887.
37. López-Cortés L, Castro D, Navas JI, Borrego JJ, (1999) Phagocytic and chemotactic responses of manila and carpet shell clam haemocytes against Vibrio tapetis, the causative agent of brown ring disease. Fish Shellfish Immunol 9: 543-555.
38. Stefano GB, Leung MK, Zhao X, Scharrer B, (1989) Evidence for the involvement of opioid neuropeptides in the adherence and migration of immunocompetent invertebrate hemocytes. Proc Natl Acad Sci USA 86: 626-630.
39. Ottaviani E, Franchini A, Kletsas D, (2001) Platelet-derived growth factor and transforming growth factor- beta in invertebrate immune and neuroendocrine interactions: another sign of conservation in evolution. Comp Biochem Physiol Part C Toxicol Pharmacol 129: 295-306.
40. Murray HM, Gallant JW, Pérez-Casanova JC, Johnson SC, Douglas SE, (2003) Ontogeny of lipase expression in winter flounder. J Fish Biol 62: 816-833.
41. Valerio PF, Kao MH, Fletcher GL, (1992) Fish skin: An effective barrier to ice crystal propagation. J Exp Biol 164: 135-151.
42. Chico V, Ortega-Villaizan M, Falcó A, Tafalla C, Pérez L, et al. (2009) The immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequences. Vaccine 27: 1938-1948.
43. De Kinkelin P, Le Berre M, (1977) Isolement d'un rhabdovirus pathogène de la truite fario (Salmo trutta, L., 1766). C R Acad Sci Hebd Seances Acad Sci D 284: 101-104.
44. Coll J, Basurco B, (1989) Variabilidad del virus de la septicemia hemorragica viral de la trucha en España. Med Vet 6: 425-430.
45. Saint-Jean SR, Pérez-Prieto SI, (2006) Interferon mediated antiviral activity against salmonid fish viruses in BF-2 and other cell lines. Vet Immunol Immunopathol 110: 1-10.
46. Lorenzo G, Estepa A, Coll JM, (1996) Fast neutralization/immunoperoxidase assay for viral haemorrhagic septicaemia with anti-nucleoprotein monoclonal antibody. J Virol Methods 58: 1-6.
47. Mas V, Pérez L, Encinar JA, Pastor MT, Rocha A, et al. (2002) Salmonid viral haemorrhagic septicaemia virus: fusion-related enhancement of virus infectivity by peptides derived from viral glycoprotein G or a combinatorial library. J Gen Virol 83: 2671-2681.
48. Reed LJ, Muench H, (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27: 493-497.
49. Brocal I, Falcó A, Mas V, Rocha A, Pérez L, et al. (2006) Stable expression of bioactive recombinant pleurocidin in a fish cell line. Appl Microbiol Biotechnol 72: 1217-1228.
50. Tafalla C, Chico V, Pérez L, Coll JM, Estepa A, (2007) In vitro and in vivo differential expression of rainbow trout (Oncorhynchus mykiss) Mx isoforms in response to viral haemorrhagic septicaemia virus (VHSV) G gene, poly I:C and VHSV. Fish Shellfish Immunol 23: 210-221.
51. Falcó A, Chico V, Marroquí L, Pérez L, Coll JM, et al. (2008) Expression and antiviral activity of a [beta]-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences. Mol Immunol 45: 757-765.
52. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408.
53. Pfaffl MW, (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45.
54. Chico V, Gómez N, Estepa A, Pérez L, (2006) Rapid detection and quantitation of viral hemorrhagic septicemia virus in experimentally challenged rainbow trout by real-time RT-PCR. J Virol Methods 132: 154-159.
55. Marroquí L, Estepa A, Perez L, (2008) Inhibitory effect of mycophenolic acid on the replication of infectious pancreatic necrosis virus and viral hemorrhagic septicemia virus. Antiviral Res 80: 332-338.
56. Raida MK, Buchmann K, (2008) Bath vaccination of rainbow trout (Oncorhynchus mykiss Walbaum) against Yersinia ruckeri: Effects of temperature on protection and gene expression. Vaccine 26: 1050-1062.