An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio

BioTechniques

Volumn 49, Issue 5, 2010, Pages 793-803

  Kodama, Yutaka ^a,b,c   Hu, Chang Deng ^a,b  

^a PURDUE UNIVERSITY   (United States)

^b Purdue University   (United States)

^c Elements Chemistry Laboratory   (Japan)

Author keywords

BiFC; Fluorescent protein; Protein protein interaction; S N ratio; Venus; Visualization

Indexed keywords

SELF ASSEMBLED MONOLAYER;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIMOLECULAR FLUORESCENCE COMPLEMENTATION ASSAY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; NONHUMAN; PROTEIN PROTEIN INTERACTION; SIGNAL NOISE RATIO; SITE DIRECTED MUTAGENESIS;

ANIMALS; BACTERIAL PROTEINS; CERCOPITHECUS AETHIOPS; COS CELLS; LUMINESCENT PROTEINS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

EID: 78349235354     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113519     Document Type: Article

References (32)

1. Heim, R. and R.Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:178-182.
2. Xu, Y., D.W. Piston, and C.H. Johnson. 1999. A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. USA 96:151-156.
3. Ghosh, I., A.D. Hamilton, and L. Regan. 2000. Antiparallel leucine zipper-directed protein reassembly: applications to the green fluorescent protein. J. Am. Chem. Soc. 122:5658-5659.
4. Hu, C.-D., Y. Chinenov, and T.K. Kerppola. 2002. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell 9:789-798.
5. Shyu, Y.J., H. Liu, X. Deng, and C.-D. Hu. 2006. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. BioTechniques 40:61-66.
6. Nagai, T., K. Ibata, E.S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20:87-90.
7. Hu, C.-D. and T.K. Kerppola. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat. Biotechnol. 21:539-545.
8. Jach, G., M. Pesch, K. Richter, S. Frings, and J.F. Uhrig. 2006. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nat. Methods 3:597-600.
9. Fan, J.-Y., Z.-Q. Cui, H.-P. Wei, Z.-P. Zhang, Y.-F. Zhou, Y.-P. Wang, and X.-E. Zhang. 2008. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochem. Biophys. Res. Commun. 367:47-53.
10. Kodama, Y. and M. Wada. 2009. Simultaneous visualization of two protein complexes in a single plant cell using multicolor fluorescence complementation analysis. Plant Mol. Biol. 70:211-217.
11. Chu, J., Z. Zhang, Y. Zheng, J. Yang, L. Qin, J. Lu, Z.L. Huang, S. Zeng, and Q. Luo. 2009. A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions. Biosens. Bioelectron. 25:234-239.
12. Lee, Y.R., J.H. Park, S.H. Hahm, L.W. Kang, J.H. Chung, K.H. Nam, K.Y. Hwang, I.C. Kwon, and Y.S. Han. 2010. Development of bimolecular fluorescence complementation using dronpa for visualization of protein-protein interactions in cells. Mol. Imaging Biol. 12:468-478.
13. Kerppola, T.K. 2008. Bimolecular f luorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annu. Rev. Biophys. 37:465-487.
14. Shyu, Y.J. and C.-D. Hu. 2008. Fluorescence complementation: an emerging tool for biological research. Trends Biotechnol. 26:622-630.
15. Shyu, Y.J., C.D. Suarez, and C.-D. Hu. 2008. Visualization of AP-1 NF-kappaB ternary complexes in living cells by using a BiFC-based FRET. Proc. Natl. Acad. Sci. USA 105:151-156.
16. Waadt, R., L.K. Schmidt, M. Lohse, K. Hashimoto, R. Bock, and J. Kudla. 2008. Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J. 56:505-516.
17. Saka, Y., A.I. Hagemann, O. Piepenburg, and J.C. Smith. 2007. Nuclear accumulation of Smad complexes occurs only after the midblastula transition in Xenopus. Development 134:4209-4218.
18. Ali, S.A. and A. Steinkasserer. 1995. PCR-ligation-PCR (PLP) mutagenesis: a protocol for creating gene fusions and mutations. BioTechniques 18:746-750.
19. Gentz, R., F.J. Rauscher 3rd, C. Abate, and T. Curran. 1989. Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains. Science 243:1695-1699.
20. Rizzo, M.A., G.H. Springer, B. Granada, and D.W. Piston. 2004. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22:445-449.
21. Rekas, A., J.R. Alattia, T. Nagai, A. Miyawaki, and M. Ikura. 2002. Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J. Biol. Chem. 277:50573-50578.
22. Pace, C.N., B.A. Shirley, M. McNutt, and K. Gajiwala. 1996. Forces contributing to the conformational stability of proteins. FASEB J. 10:75-83.
23. Williams, R.W., A. Chang, D. Juretic, and S. Loughran. 1987. Secondary structure predictions and medium range interactions. Biochim. Biophys. Acta 916:200-204.
24. Kyte, J. and R.F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
25. Robida, A.M. and T.K. Kerppola. 2009. Bimolecular f luorescence complementation analysis of inducible protein interactions: effects of factors affecting protein folding on fluorescent protein fragment association. J. Mol. Biol. 394:391-409.
26. Hiatt, S.M., Y.J. Shyu, H.M. Duren, and C.-D. Hu. 2008. Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in Caenorhabditis elegans. Methods 45:185-191.
27. Remy, I. and S.W. Michnick. 2006. A highly sensitive proteinprotein interaction assay based on Gaussia luciferase. Nat. Methods 3:977-979.
28. Magliery, T.J., C.G.M. Wilson, W. Pan, D. Mishler, and L. Regan. 2005. Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J. Am. Chem. Soc. 127:146-157.
29. Cabantous, S., T.C. Terwilliger, and G.S. Waldo. 2005. Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nat. Biotechnol. 23:102-107.
30. Demidov, V.V., N.V. Dokholyan, C. Witte-Hoffmann, P. Chalasani, H.W. Yiu, F. Ding, Y. Yu, C.R. Cantor, and N.E. Broude. 2006. Fast complementation of split fluorescent protein triggered by DNA hybridization. Proc. Natl. Acad. Sci. USA 103:2052-2056.
31. Malo, G.D., L.J. Pouwels, M. Wang, A. Weichsel, W.R. Montfort, M.A. Rizzo, D.W. Piston, and R.M. Wachter. 2007. X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging. Biochemistry 46:9865-9873.
32. Lin, J., N. Wang, Y. Li, Z. Liu, S. Tian, L. Zhao, Y. Zheng, S. Liu, et al. 2010. LEC-BiFC: a new method for rapid assay of protein interaction. Biotech. Histochem. May 14. [Epub ahead of print].