Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence

Applied Microbiology and Biotechnology

Volumn 91, Issue 3, 2011, Pages 731-740

  Wang, Jia Ye ^a,b   Song, Wen Ting ^a   Li, Yan ^a   Chen, Wen Jiang ^a   Yang, Dan ^a   Zhong, Guo Cai ^a,c   Zhou, Hai Zhou ^a   Ren, Cai Yun ^a   Yu, Hao Tong ^a   Ling, Hong ^a,b  

^a HARBIN MEDICAL UNIVERSITY   (China)

^b Key Lab of Heilongjiang Province Education Bureau for Etiology   (China)

^c NATIONAL INSTITUTE OF BIOLOGICAL SCIENCES   (China)

Author keywords

Mammalian cell; Protein expression; Signal peptide; Tissue plasminogen activator (tPA); Trimer motif

Indexed keywords

MAMMALIAN CELLS; PROTEIN EXPRESSION; SIGNAL PEPTIDE; TISSUE PLASMINOGEN ACTIVATOR (TPA); TRIMER MOTIF;

MAMMALS; PEPTIDES; TISSUE;

OLIGOMERS;

GLYCOPROTEIN GP 120; PLASMID VECTOR; PLASMID VECTOR MTI; PLASMID VECTOR MTQ; SECRETORY PROTEIN; SIGNAL PEPTIDE; TISSUE PLASMINOGEN ACTIVATOR; UNCLASSIFIED DRUG;

ANTIGEN; CELL ORGANELLE; GENE EXPRESSION; GENETIC ANALYSIS; IMMUNE SYSTEM; MAMMAL; PEPTIDE; PLASMID; PROTEIN; SECRETION; SIGNAL;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; RECOMBINANT PLASMID; WESTERN BLOTTING;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; HEK293 CELLS; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HIV; HUMANS; INFLUENZA A VIRUS, H3N2 SUBTYPE; MULTIPROTEIN COMPLEXES; MUTATION; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; TISSUE PLASMINOGEN ACTIVATOR; VACCINES, DNA; VACCINES, SUBUNIT;

MAMMALIA;

EID: 79960722461     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3297-0     Document Type: Article

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