A buried polar residue in the hydrophobic interface of the coiled-coil peptide, GCN4-p1, plays a thermodynamic, not a kinetic role in folding

Journal of Molecular Biology

Volumn 321, Issue 1, 2002, Pages 1-6

  Knappenberger, Jane A ^a   Smith, Jennifer E ^a   Thorpe, Sarah H ^a   Zitzewitz, Jill A ^a,b   Matthews, C Robert ^a,b  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Buried polar residues; Circular dichroism spectroscopy; Leucine zipper peptides; Protein folding kinetics

Indexed keywords

ALANINE; DIMER; LEUCINE ZIPPER PROTEIN; M PROTEIN; PEPTIDE DERIVATIVE; VALINE;

ARTICLE; DISSOCIATION; HYDROPHOBICITY; KINETICS; MUTATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FOLDING; THERMODYNAMICS;

EID: 0036353719     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00592-2     Document Type: Article

References (21)

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3. Ion-pairs in proteins
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6. Predicting oligomerization states of coiled coils
7. A buried polar interaction imparts structural uniqueness in a designed heterodimeric coiled coil
8. Buried polar residues and structural specificity in the GCN4 leucine zipper
9. Buried asparagines determine the dimerization specificities of leucine zipper mutants
10. A buried polar interaction can direct the relative orientation of helices in a coiled coil
11. Evidence that the leucine zipper is a coiled coil
12. Probing the folding mechanism of a leucine zipper peptide by stopped-flow circular dichroism
13. Transition state heterogeneity in GCN4 coiled coil folding studied using multiple mutations and cross-linking
14. An autonomous folding unit mediates the assembly of two-stranded coiled coils
15. The contribution of buried polar groups to the conformational stability of the GCN4 coiled coil
16. Temperature dependence of the folding and unfolding kinetics of the GCN4 leucine zipper via 13C(alpha)-NMR
17. X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil
18. Synthesis and properties of the peptide corresponding to the mutant form of the leucine zipper of the transcriptional activator GCN4 from yeast
19. An engineered allosteric switch in leucine-zipper oligomerization
20. Mapping the energy surface for the folding reaction of the coiled-coil peptide of GCN4-p1
21. Preformed secondary structure drives the association reaction of GCN4-p1, a model coiled-coil system