Impedance-controlled cell entrapment using microhole-array chips allows the isolation and identification of single, highly productive cells

Sensors and Actuators, B: Chemical

Volumn 158, Issue 1, 2011, Pages 345-352

  Kurz, Christian M ^a   Maurer, Andrea ^a   Thees, Katrin ^b   Schillberg, Stefan ^b   Velten, Thomas ^a   Thielecke, Hagen ^a  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

Cell micro arrays; Highly productive cell; Image processing; Impedance spectroscopy; Pattern occupancy

Indexed keywords

ANTIBODY DETECTION; BIOPHARMACEUTICAL PROTEINS; CAPTURE CELLS; CELL POSITIONING; DIRECT ILLUMINATION; FLUORESCENCE IMAGING; FLUORESCENT REAGENT; HIGHLY PRODUCTIVE CELL; IMAGE PROCESSING ALGORITHM; IMPEDANCE SPECTROSCOPY; INDIVIDUAL CELLS; ISOLATION AND IDENTIFICATION; MICRO HOLES; OPTICAL ANALYSIS; PATTERN OCCUPANCY; SEMI-SOLIDS;

ANTIBODIES; CHEMICAL DETECTION; CYTOLOGY; FLUORESCENCE; IMAGE PROCESSING; IMAGING SYSTEMS; OPTICAL DATA PROCESSING; PHYSIOLOGY;

CELLS;

EID: 79960449663     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.snb.2011.06.033     Document Type: Article

References (22)

1. S.M. Browne, and M. Al-Rubeai Selection methods for high-producing mammalian cell lines Trends Biotechnol. 25 2007 425 432 (Pubitemid 350147111)
2. T.T. Puck, and P.I. Marcus A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors Proc. Natl. Acad. Sci. U.S.A. 41 1955 432 437
3. C. Lee, C. Ly, T. Sauerwald, T. Kelly, and G. Moore High-throughput screening of cell lines expressing monoclonal antibodies Bioprocess Int. 4 2006 32 35
4. J.M. Davis Basic Cell Culture - A Practical Approach second ed. 2002 University Press Oxford
5. P.A. Underwood, and P.A. Bean Hazards of the limiting-dilution methods of cloning hybridomas J. Immunol. Methods 107 1987 119 128 (Pubitemid 18060375)
6. H.A. Coller, and B.S. Coller Poisson statistical analysis of repetitive sub-cloning by the limiting dilution technique as a way of assessing hybridoma monoclonality Methods Enzymol. 121 1986 412 417 (Pubitemid 16057915)
7. F. Mancia, S.D. Patel, M.W. Rajala, P.E. Scherer, A. Nemes, I. Schieren, W.A. Hendrickson, and L. Shapiro Optimization of protein production in mammalian cells with a coexpressed fluorescent marker Structure 12 2004 1355 1360 (Pubitemid 39092080)
8. R. Manz, M. Assenmacher, E. Pfluger, S. Milteny, and A. Radbruch Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix Proc. Natl. Acad. Sci. U.S.A. 92 1995 1921 1925
9. P. Holmes, and M. Al-Rubeai Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors J. Immunol. Methods 230 1999 141 147
10. N. Borth, M. Zeyda, R. Kunert, and H. Katinger Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting Biotechnol. Bioeng. 71 2000 266 273 (Pubitemid 32374469)
11. M. Hosokawa, A. Arakaki, M. Takahashi, T. Mori, H. Takeyama, and T. Matsunaga High-density microcavity array for cell detection: single-cell analysis of hematopoietic stem cells in peripheral blood mononuclear cells Anal. Chem. 81 2009 5308 5313
12. J.R. Rettig, and A. Forch Large-scale single-cell trapping and imaging using microwell arrays Anal. Chem. 77 2005 5628 5634 (Pubitemid 41266059)
13. J.R. Kovac, and J. Voldman Intuitive, image-based cell sorting using optofluidic cell sorting Anal. Chem. 79 2007 9321 9330 (Pubitemid 350276310)
14. S. Tatic-Lucic, J.A. Wright, Y.-C. Tai, and J. Pine Silicon cultured-neuron prosthetic devices for in vivo and in vitro studies Sens. Actuators B: Chem. 43 1997 105 109 (Pubitemid 127406136)
15. E. Ostuni, R. Kane, C.S. Chen, D.E. Ingber, and G.M. Whitesides Patterning mammalian cells using elastomeric membranes Langmuir 2000 7811 7819
16. C.M. Kurz, and H. Thielecke Characterisation of the deflection of thin perforated SiN membranes of micro hole array chips used for the cell selection Microelectron. Eng. 2011
17. C.M. Kurz, H. Büth, and H. Thielecke Influence of transfection process on single cell impedance J. Phys.: Conf. Ser. 224 2010
18. S. Yamamura, H. Kishi, Y. Tokimitsu, S. Kondo, R. Honda, S.R. Rao, M. Omori, E. Tamiya, and A. Muraguchi Single-cell microarray for analyzing cellular response Anal. Chem. 77 2005 8050 8056 (Pubitemid 41831992)
19. C.M. Kurz, H. Büth, A. Sossalla, V. Vermeersch, V. Toncheva, P. Dubruel, E. Schacht, and H. Thielecke Chip-based impedance measurement on single cells for monitoring sub-toxic effects on cell membranes Biosens. Bioelectron. 2011
20. S.B. Prakash, and P.A. Abshire On-chip capacitance sensing for cell monitoring applications IEEE Sens. J. 7 2007 440 447
21. S. Gawad, L. Schild, and Ph. Renaud Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing Lab. Chip 1 2001 76 82
22. S. Cho Electrical resistance characterization of insulating membrane with multipore J. Electroanal. Chem. 629 2009 69 72