Export is the default pathway for soluble unfolded polypeptides that accumulate during expression in Escherichia coli

Protein Expression and Purification

Volumn 79, Issue 1, 2011, Pages 137-141

  Scotto Lavino, Elizabeth ^a,b   Bai, Mei ^a,c   Zhang, Yian Biao ^a   Freimuth, Paul ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b STONY BROOK UNIVERSITY MEDICAL CENTER   (United States)

^c Occhiuti Rohlicek and Tsao   (United States)

Author keywords

Export; GroEL; Molecular chaperones; Overexpression; Protein folding

Indexed keywords

CHAPERONIN; ESCHERICHIA COLI PROTEIN; PEPTIDE; VIRUS PROTEIN;

ADENOVIRUS; ARTICLE; CYTOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN TRANSPORT; PROTEIN UNFOLDING; SOLUBILITY; UPREGULATION;

ADENOVIRIDAE; CHAPERONIN 60; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; PEPTIDES; PROTEIN TRANSPORT; PROTEIN UNFOLDING; SOLUBILITY; UP-REGULATION; VIRAL PROTEINS;

ESCHERICHIA COLI;

EID: 79960137219     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.03.011     Document Type: Article

References (32)

1. F.W. Studier, and B.A. Moffatt Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes J. Mol. Biol. 189 1986 113 130
2. F.U. Hartl, and M. Hayer-Hartl Converging concepts of protein folding in vitro and in vivo Nat. Struct. Mol. Biol. 16 2009 574 581
3. M.J. Kerner, D.J. Naylor, Y. Ishihama, T. Maier, H.C. Chang, A.P. Stines, C. Georgopoulos, D. Frishman, M. Hayer-Hartl, and F.U. Hartl Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli Cell 122 2005 209 220 (Pubitemid 41032985)
4. Z. Xu, A.L. Horwich, and P.B. Sigler The crystal structure of the asymmetric GroEL-GroES-(ADP)7 chaperonin complex Nature 388 1997 741 750 (Pubitemid 27375147)
5. M. Martinez-Alonso, E. Garcia-Fruitos, N. Ferrer-Miralles, U. Rinas, and A. Villaverde Side effects of chaperone gene co-expression in recombinant protein production Microb. Cell. Fact 9 2010 64
6. A. Gragerov, and M.E. Gottesman Different peptide binding specificities of hsp70 family members J. Mol. Biol. 241 1994 133 135 (Pubitemid 24263419)
7. A.M. Fourie, J.F. Sambrook, and M.J. Gething Common and divergent peptide binding specificities of hsp70 molecular chaperones J. Biol. Chem. 269 1994 30470 30478 (Pubitemid 24376527)
8. G.H. Lorimer A quantitative assessment of the role of the chaperonin proteins in protein folding in vivo Faseb. J. 10 1996 5 9 (Pubitemid 26035500)
9. W.A. Houry, D. Frishman, C. Eckerskorn, F. Lottspeich, and F.U. Hartl Identification of in vivo substrates of the chaperonin GroEL Nature 402 1999 147 154
10. M.J. van Raaij, N. Louis, J. Chroboczek, and S. Cusack Structure of the human adenovirus serotype 2 fiber head domain at 1.5 A resolution Virology 262 1999 333 343 (Pubitemid 29474296)
11. A.H. Rosenberg, B.N. Lade, D.S. Chui, S.W. Lin, J.J. Dunn, and F.W. Studier Vectors for selective expression of cloned DNAs by T7 RNA polymerase Gene 56 1987 125 135
12. T. Langer, C. Lu, H. Echols, J. Flanagan, M.K. Hayer, and F.U. Hartl Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding Nature 356 1992 683 689
13. G. Blobel, and B. Dobberstein Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma J. Cell. Biol. 67 1975 835 851
14. G. Blobel, and B. Dobberstein Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components J. Cell. Biol. 67 1975 852 862
15. D.T. Ng, J.D. Brown, and P. Walter Signal sequences specify the targeting route to the endoplasmic reticulum membrane J. Cell. Biol. 134 1996 269 278 (Pubitemid 26250697)
16. P. Bassford, J. Beckwith, K. Ito, C. Kumamoto, S. Mizushima, D. Oliver, L. Randall, T. Silhavy, P.C. Tai, and B. Wickner The primary pathway of protein export in E. Coli Cell 65 1991 367 368 (Pubitemid 121002046)
17. L.L. Randall Translocation of domains of nascent periplasmic proteins across the cytoplasmic membrane is independent of elongation Cell 33 1983 231 240 (Pubitemid 13096452)
18. Y.R. Thorstenson, Y. Zhang, P.S. Olson, and D. Mascarenhas Leaderless polypeptides efficiently extracted from whole cells by osmotic shock J. Bacteriol. 179 1997 5333 5339 (Pubitemid 27387600)
19. C.A. Kumamoto Escherichia coli SecB protein associates with exported protein precursors in vivo Proc. Natl. Acad. Sci. USA 86 1989 5320 5324 (Pubitemid 19185545)
20. P.J. Bassford Jr. Export of the periplasmic maltose-binding protein of Escherichia coli J. Bioenerg. Biomembr. 22 1990 401 439 (Pubitemid 20192843)
21. H.C. Neu, and L.A. Heppel The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts J. Biol. Chem. 240 1965 3685 3692
22. C. Zubieta, G. Schoehn, J. Chroboczek, and S. Cusack The structure of the human adenovirus 2 penton Mol. Cell. 17 2005 121 135 (Pubitemid 40075371)
23. P.L. Stewart, C.Y. Chiu, S. Huang, T. Muir, Y. Zhao, B. Chait, P. Mathias, and G.R. Nemerow Cryo-EM visualization of an exposed RGD epitope on adenovirus that escapes antibody neutralization Embo. J. 16 1997 1189 1198 (Pubitemid 27136086)
24. J.D. Bendtsen, H. Nielsen, G. von Heijne, and S. Brunak Improved prediction of signal peptides: SignalP 3.0 J. Mol. Biol. 340 2004 783 795 (Pubitemid 38829638)
25. E. Joseph-Liauzun, P. Leplatois, R. Legoux, V. Guerveno, E. Marchese, and P. Ferrara Human recombinant interleukin-1 beta isolated from Escherichia coli by simple osmotic shock Gene 86 1990 291 295 (Pubitemid 20098919)
26. M.C. Frate, E.J. Lietz, J. Santos, J.P. Rossi, A.L. Fink, and M.R. Ermacora Export and folding of signal-sequenceless Bacillus licheniformis beta-lactamase in Escherichia coli Eur. J. Biochem. 267 2000 3836 3847 (Pubitemid 30423047)
27. B.H. Johnson, and M.H. Hecht Recombinant proteins can be isolated from E. Coli cells by repeated cycles of freezing and thawing Biotechnology (NY) 12 1994 1357 1360 (Pubitemid 2165237)
28. P. Demchick, and A.L. Koch The permeability of the wall fabric of Escherichia coli and Bacillus subtilis J. Bacteriol. 178 1996 768 773 (Pubitemid 26036648)
29. P.J. Bassford Jr., T.J. Silhavy, and J.R. Beckwith Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm J. Bacteriol. 139 1979 19 31 (Pubitemid 9227488)
30. A. Guigueno, J. Dassa, P. Belin, and P.L. Boquet Oversynthesis of a new Escherichia coli small RNA suppresses export toxicity of DsbA'-PhoA unfoldable periplasmic proteins J. Bacteriol. 183 2001 1147 1158 (Pubitemid 32107710)
31. B. Bukau, J. Weissman, and A. Horwich Molecular chaperones and protein quality control Cell 125 2006 443 451
32. K.C. Keiler, P.R. Waller, and R.T. Sauer Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA Science 271 1996 990 993 (Pubitemid 26066397)