Characterization of human OCT1-mediated transport of DAPI as a fluorescent probe substrate

Journal of Pharmaceutical Sciences

Volumn 100, Issue 9, 2011, Pages 4006-4012

  Yasujima, Tomoya ^a   Ohta, Kinya ^a   Inoue, Katsuhisa ^a   Yuasa, Hiroaki ^a  

^a NAGOYA CITY UNIVERSITY   (Japan)

Author keywords

DAPI; Drug interactions; Hepatic transport; MDCK cells; Membrane transporter; OCT; Screening; TEA

Indexed keywords

4 AMINOBUTYRIC ACID; 4',6 DIAMIDINO 2 PHENYLINDOLE; CATECHIN; CHLORAMPHENICOL; COLCHICINE; CORTICOSTERONE; DESIPRAMINE; DILTIAZEM; DIPHENHYDRAMINE; DOPAMINE; FLUCONAZOLE; GLIBENCLAMIDE; HEMICHOLINIUM 3; N METHYLNICOTINAMIDE; NICOTINAMIDE; NICOTINIC ACID; NORADRENALIN; OCTAMER TRANSCRIPTION FACTOR 1; PENICILLIN G; PERPHENAZINE; PHENYTOIN; PROGESTERONE; PROPANTHELINE BROMIDE; PROPRANOLOL; PYRIDOXINE; QUERCETIN; TETRYLAMMONIUM BROMIDE; TRIMETHOPRIM; UNINDEXED DRUG; VERAPAMIL;

ANIMAL CELL; ARTICLE; CELL PH; CONTROLLED STUDY; DRUG TRANSPORT; DRUG UPTAKE; EXPERIMENTAL DOG; FLUORESCENCE; GENETIC TRANSFECTION; HUMAN; KIDNEY CELL; MEMBRANE POTENTIAL; MICHAELIS CONSTANT; NONHUMAN; PROTEIN EXPRESSION;

EID: 79960120117     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.22548     Document Type: Article

References (25)

1. Gorboulev V, Ulzheimer JC, Akhoundova A, Ulzheimer-Teuber I, Karbach U, Quester S, Baumann C, Lang F, Busch AE, Koepsell H. 1997. Cloning and characterization of two human polyspecific organic cation transporters. DNA Cell Biol 16:871-881.
2. Zhang L, Dresser MJ, Gray AT, Yost SC, Terashita S, Giacomini KM. 1997. Cloning and functional expression of a human liver organic cation transporter. Mol Pharmacol 51:913-921.
3. Ciarimboli G. 2008. Organic cation transporters. Xenobiotica 38:936-971.
4. Wright SH, Dantzler WH. 2004. Molecular and cellular physiology of renal organic cation and anion transport. 84:987-1049.
5. Koepsell H. 2004. Polyspecific organic cation transporters: Their functions and interactions with drugs. Trends Pharmacol Sci 25:375-381.
6. Jonker JW, Schinkel AH. 2004. Pharmacological and physiological functions of the polyspecific organic cation transporters: OCT1, 2, and 3 (SLC22A1-3). J Pharmacol Exp Ther 308:2-9.
7. Yasujima T, Ohta K, Inoue K, Ishimaru M, Yuasa H. 2010. Evaluation of 4', 6-diamidino-2-phenylindole as a fluorescent probe substrate for rapid assays of the functionality of human multidrug and toxin extrusion proteins. Drug Metab Dispos 38:715-721.
8. Kapuscinski J. 1995. DAPI: A DNA-specific fluorescent probe. Biotech Histochem 70:220-233.
9. Ahlin G, Karlsson J, Pedersen JM, Gustavsson L, Larsson R, Matsson P, Norinder U, Bergström CA, Artursson P. 2008. Structural requirements for drug inhibition of the liver specific human organic cation transport protein 1. J Med Chem 51:5932-5942.
10. Bednarczyk D, Mash EA, Aavula BR, Wright SH. 2000. NBD-TMA: A novel fluorescent substrate of the peritubular organic cation transporter of renal proximal tubules. Pflugers Arch 440:184-192.
11. Nies AT, Herrmann E, Brom M, Keppler D. 2008. Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1). Naunyn Schmiedebergs Arch Pharmacol 376:449-461.
12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275.
13. Gründemann D, Gorboulev V, Gambaryan S, Veyhl M, Koepsell H. 1994. Drug excretion mediated by a new prototype of polyspecific transporter. Nature 372:549-552.
14. Zhang L, Schaner ME, Giacomini KM. 1998. Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa). J Pharmacol Exp Ther 286;354-361.
15. Takami K, Saito H, Okuda M, Takano M, Inui KI. 1998. Distinct characteristics of transcellular transport between nicotine and tetraethylammonium in LLC-PK1 cells. J Pharmacol Exp Ther 286:676-680.
16. + antiporter in the kidney. Drug Metab Dispos 34:1868-1874.
17. Ohta K, Inoue K, Yasujima T, Ishimaru M, Yuasa H. 2009. Functional characteristics of two human MATE transporters: Kinetics of cimetidine transport and profiles of inhibition by various compounds. J Pharm Pharmceut Sci 12:388-396.
18. Otsuka M, Matsumoto T, Morimoto R, Arioka S, Omote H, Moriyama Y. 2005. A human transporter protein that mediates the final excretion step for toxic organic cations. Proc Natl Acad Sci USA 13:17923-17928.
19. +/organic cation antiporter, kidney-specific multidrug and toxin extrusion 2. J Am Soc Nephrol 17:2127-2135.
20. Ohta K, Imamura Y, Okudaira N, Atsumi R, Inoue K, Yuasa H. 2009. Functional characterization of multidrug and toxin extrusion protein 1 as a facilitative transporter for fluoroquinolones. J Pharmacol Exp Ther 328:628-634.
21. Tamai I, Ohashi R, Nezu J, Yabuuchi H, Oku A, Shimane M, Sai Y, Tsuji A. 1998. Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2. J Biol Chem 273:20378-20382.
22. Wu X, Huang W, Prasad PD, Seth P, Rajan DP, Leibach FH, Chen J, Conway SJ, Ganapathy V. 1999. Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. J Pharmacol Exp Ther 290:1482-1492.
23. Miyaji T, Echigo N, Hiasa M, Senoh S, Omote H, Moriyama Y. 2008. Identification of a vesicular aspartate transporter. Proc Natl Acad Sci USA 105:11720-11724.
24. Solimani R. 1996. Quercetin and DNA in solution: Analysis of the dynamics of their interaction with a linear dichroism study. Int J Biol Macromol 18:287-295.
25. Liu J, Wang ZH, Luo GA, Li QW, Sun HW. 2002. The intercalation of DNA with dopamine by spectroscopic and electrochemical methods. Anal Sci 18:751-755.