Phosphoproteome profiling using a fluorescent phosphosensor dye in two-dimensional polyacrylamide gel electrophoresis

Applied Biochemistry and Biotechnology

Volumn 164, Issue 6, 2011, Pages 804-818

  Otani, Mieko ^a   Taniguchi, Taizo ^b,e   Sakai, Akiko ^c   Seta, Jouji ^d   Kadoyama, Keiichi ^e   Nakamura Hirota, Tooru ^e   Matsuyama, Shogo ^e   Sano, Keiji ^a   Takano, Masaoki ^a  

^a KOBE GAKUIN UNIVERSITY   (Japan)

^b Behavioral and Medical Sciences Research Consortium   (Japan)

^c OSAKA MEDICAL COLLEGE   (Japan)

^d BRUKER DALTONIK GMBH   (Germany)

^e HIMEJI DOKKYO UNIVERSITY   (Japan)

Author keywords

MALDI TOF MS; Phosphoproteome; PhosphoQUANTI solidblue complex; Swiss 3T3 cell; Two dimensional PAGE

Indexed keywords

MALDI TOF-MS; PHOSPHOPROTEOME; PHOSPHOQUANTI SOLIDBLUE COMPLEX; SWISS 3T3 CELL; TWO-DIMENSIONAL PAGE;

DESORPTION; ELECTROPHORESIS; FLUORESCENCE; GELS; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETRY; PHOSPHORYLATION; SIGNAL DETECTION; TWO DIMENSIONAL;

PROTEINS;

BETA CASEIN; FLUORESCENT DYE; OVALBUMIN; PHOSPHOPROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOQUANTI SOLIDBLUE COMPLEX DYE; UNCLASSIFIED DRUG;

ARTICLE; CELL PROLIFERATION; CELL SEPARATION; CELL STRAIN 3T3; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEOMICS; SIGNAL TRANSDUCTION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

3T3 CELLS; ANIMALS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLUORESCENT DYES; MICE; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEINS; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79960078033     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9175-5     Document Type: Article

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