Expression of tung tree diacylglycerol acyltransferase 1 in E. coli

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Cao, Heping ^a   Chapital, Dorselyn C ^a   Shockey, Jay M ^a   Klasson, K Thomas ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY TAGS; BACTERIAL EXPRESSION SYSTEMS; CARBOXYL TERMINUS; DATABASE SEARCHES; DIACYLGLYCEROL ACYLTRANSFERASES; E. COLI; EUKARYOTIC ORGANISMS; FULL-LENGTH PROTEINS; FUSION PROTEINS; IMMUNOBLOTTING; INTEGRAL MEMBRANE PROTEINS; MALTOSE BINDING PROTEINS; OPEN READING FRAME; PROTEASE DIGESTION; RATE-LIMITING STEPS; SOLUBLE FRACTION; TRIACYLGLYCEROLS; TRITON X-100; VERNICIA FORDII;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BIOCHEMISTRY; BIOLOGICAL MEMBRANES; ESCHERICHIA COLI; FATTY ACIDS; GLYCEROL; PLANT EXTRACTS; PURIFICATION; SEARCH ENGINES; SOAPS (DETERGENTS); UREA;

PROTEINS;

DIACYLGLYCEROL ACYLTRANSFERASE 1; FATTY ACID; HISTIDINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; TRITON X 100; UREA; DETERGENT; DIACYLGLYCEROL ACYLTRANSFERASE; POLYHISTIDINE; VEGETABLE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; DEGRADATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; IMMUNOBLOTTING; NONHUMAN; PROTEIN EXPRESSION; ALEURITES; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY;

BACTERIA (MICROORGANISMS); EUKARYOTA; VERNICIA FORDII;

ALEURITES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DETERGENTS; DIACYLGLYCEROL O-ACYLTRANSFERASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; IMMUNOBLOTTING; MALTOSE-BINDING PROTEINS; PHYLOGENY; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; UREA;

EID: 79960032578     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-73     Document Type: Article

References (35)

1. Cao H, Dzineku F, Blackshear PJ. Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases. Arch Biochem Biophys 2003, 412:106-120. 10.1016/S0003-9861(03)00012-2, 1351391, 12646273.
2. Cao H. Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications. Biochemistry 2004, 43:13724-13738. 10.1021/bi049014y, 1351390, 15504035.
3. Cao H, Tuttle JS, Blackshear PJ. Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein. J Biol Chem 2004, 279:21489-21499. 10.1074/jbc.M400900200, 1351392, 15010466.
4. Cao H, Lin R, Ghosh S, Anderson RA, Urban JF. Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli. Biotechnol Prog 2008, 24:326-333. 10.1021/bp070269n, 2674335, 18302406.
5. Cao H. Recombinant protein production technology. J Jiangxi Agric Univ 2010, 32:1018-1031.
6. Cao H. Bioengineering recombinant diacylglycerol acyltransferases. Bioengineering 2011, 1-16. Rijeka, Croatia: InTech, Carpi A.
7. Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Lusis AJ, Erickson SK, Farese RV. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci USA 1998, 95:13018-13023. 10.1073/pnas.95.22.13018, 23692, 9789033.
8. Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J Biol Chem 2001, 276:38870-38876. 10.1074/jbc.M106219200, 11481335.
9. Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, Hawkins DJ. DGAT2 is a new diacylglycerol acyltransferase gene family: purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity. J Biol Chem 2001, 276:38862-38869. 10.1074/jbc.M106168200, 11481333.
10. Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM. Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 2006, 18:2294-2313. 10.1105/tpc.106.043695, 1560902, 16920778.
11. Durrett TP, McClosky DD, Tumaney AW, Elzinga DA, Ohlrogge J, Pollard M. A distinct DGAT with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in Euonymus and transgenic seeds. Proc Natl Acad Sci USA 2010, 107:9464-9469. 10.1073/pnas.1001707107, 2889089, 20439724.
12. Saha S, Enugutti B, Rajakumari S, Rajasekharan R. Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase. Plant Physiol 2006, 141:1533-1543. 10.1104/pp.106.082198, 1533943, 16798944.
13. Stone SJ, Myers HM, Watkins SM, Brown BE, Feingold KR, Elias PM, Farese RV. Lipopenia and skin barrier abnormalities in DGAT2-deficient mice. J Biol Chem 2004, 279:11767-11776. 10.1074/jbc.M311000200, 14668353.
14. Chen HC, Smith SJ, Ladha Z, Jensen DR, Ferreira LD, Pulawa LK, McGuire JG, Pitas RE, Eckel RH, Farese RV. Increased insulin and leptin sensitivity in mice lacking acyl CoA:diacylglycerol acyltransferase 1. J Clin Invest 2002, 109:1049-1055. 150948, 11956242.
15. Smith SJ, Cases S, Jensen DR, Chen HC, Sande E, Tow B, Sanan DA, Raber J, Eckel RH, Farese RV. Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat. Nat Genet 2000, 25:87-90. 10.1038/75651, 10802663.
16. Stone SJ, Levin MC, Farese RV. Membrane topology and identification of key functional amino acid residues of murine acyl-CoA:diacylglycerol acyltransferase-2. J Biol Chem 2006, 281:40273-40282. 10.1074/jbc.M607986200, 17035227.
17. Cheng D, Meegalla RL, He B, Cromley DA, Billheimer JT, Young PR. Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein. Biochem J 2001, 359:707-714. 10.1042/0264-6021:3590707, 1222193, 11672446.
18. Weselake RJ, Madhavji M, Szarka SJ, Patterson NA, Wiehler WB, Nykiforuk CL, Burton TL, Boora PS, Mosimann SC, Foroud NA, Thibault BJ, Moloney MM, Laroche A, Furukawa-Stoffer TL. Acyl-CoA-binding and self-associating properties of a recombinant 13.3 kDa N-terminal fragment of diacylglycerol acyltransferase-1 from oilseed rape. BMC Biochem 2006, 7:24. 10.1186/1471-2091-7-24, 1764880, 17192193.
19. Siloto RM, Madhavji M, Wiehler WB, Burton TL, Boora PS, Laroche A, Weselake RJ. An N-terminal fragment of mouse DGAT1 binds different acyl-CoAs with varying affinity. Biochem Biophys Res Commun 2008, 373:350-354. 10.1016/j.bbrc.2008.06.031, 18571500.
20. Hobbs DH, Lu C, Hills MJ. Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression. FEBS Lett 1999, 452:145-149. 10.1016/S0014-5793(99)00646-8, 10386579.
21. McFie PJ, Stone SL, Banman SL, Stone SJ. Topological orientation of acyl-CoA:diacylglycerol acyltransferase-1 (DGAT1) and identification of a putative active site histidine and the role of the n terminus in dimer/tetramer formation. J Biol Chem 2010, 285:37377-37387. 10.1074/jbc.M110.163691, 20876538.
22. Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 1999, 8:1668-1674. 10.1110/ps.8.8.1668, 2144417, 10452611.
23. Cao H, Deterding LJ, Venable JD, Kennington EA, Yates JR, Tomer KB, Blackshear PJ. Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis. Biochem J 2006, 394:285-297. 10.1042/BJ20051316, 1386027, 16262601.
24. Cao H, Lin R. Phosphorylation of recombinant tristetraprolin in vitro. Protein J 2008, 27:163-169. 10.1007/s10930-007-9119-7, 2674330, 18071886.
25. Cao H, Lin R. Quantitative evaluation of His-tag purification and immunoprecipitation of tristetraprolin and its mutant proteins from transfected human cells. Biotechnol Prog 2009, 25:461-467. 10.1002/btpr.121, 2680604, 19330843.
26. Bouvier-Nave P, Benveniste P, Oelkers P, Sturley SL, Schaller H. Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase. Eur J Biochem 2000, 267:85-96. 10.1046/j.1432-1327.2000.00961.x, 10601854.
27. Christiansen K. Triacylglycerol synthesis in lipid particles from baker's yeast (Saccharomyces cerevisiae). Biochim Biophys Acta 1978, 530:78-90.
28. Kamisaka Y, Mishra S, Nakahara T. Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus. J Biochem 1997, 121:1107-1114.
29. Kuerschner L, Moessinger C, Thiele C. Imaging of lipid biosynthesis: how a neutral lipid enters lipid droplets. Traffic 2008, 9:338-352. 10.1111/j.1600-0854.2007.00689.x, 18088320.
30. Ren Q, De RG, Kessler B, Witholt B. Recovery of active medium-chain-length-poly-3-hydroxyalkanoate polymerase from inactive inclusion bodies using ion-exchange resin. Biochem J 2000, 349:599-604. 10.1042/0264-6021:3490599, 1221183, 10880359.
31. Massie HR, Zimm BH. Molecular weight of the DNA in the chromosomes of E. coli and B. subtilis. Proc Natl Acad Sci USA 1965, 54:1636-1641. 10.1073/pnas.54.6.1636, 300526, 4956451.
32. Romanov GA, Spichal L, Lomin SN, Strnad M, Schmulling T. A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein. Anal Biochem 2005, 347:129-134. 10.1016/j.ab.2005.09.012, 16246292.
33. Cao H, Sullivan TD, Boyer CD, Shannon JC. Bt1, a structural gene for the major 39-44 kDa amyloplast membrane polypeptides. Physiol Plant 1995, 95:176-186.
34. Cao H, Chapital DC, Howard OD, Jiang XN, Shockey JM, Klasson KT. Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli. FASEB J 2011, 25:765-8. 10.1096/fj.10-166595, 20959516.
35. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4:406-425.