Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase

Plant Physiology

Volumn 141, Issue 4, 2006, Pages 1533-1543

  Saha, Saikat ^a   Enugutti, Balaji ^a   Rajakumari, Sona ^a   Rajasekharan, Ram ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; COLUMN CHROMATOGRAPHY; DNA; GLYCEROL; PLANT CELL CULTURE;

CYTOSOLIC FRACTION; EUKARYOTIC CELLS; OILSEEDS; TRIACYLGLYCEROLS (TAGS);

AGRICULTURAL PRODUCTS;

BIOSYNTHESIS; COLUMN CHROMATOGRAPHY; GLYCEROL; NUCLEIC ACIDS; PLANTS;

ARACHIS HYPOGAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 33747123328     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.106.082198     Document Type: Article

References (28)

1. Bouvier-Nave P, Benveniste P, Oelkers P, Sturley SL, Schaller H (2000) Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglycerol acyltransferase. Eur J Biochem 267: 85-96
2. Broun P, Gettner S, Somerville C (1999) Genetic engineering of plant lipids. Annu Rev Nutr 19: 197-216
3. Cases S, Smith SJ, Zheng Y-W, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Lusis AJ, et al (1998) Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci USA 95: 13018-13023
4. Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne S (2000) Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97: 6487-6492
5. Daniel J, Deb C, Dubey VS, Sirakova TD, Abomoelak B, Morbidoni HR, Kolattukudy PE (2004) Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture. J Bacteriol 186: 5017-5030
6. Gangar A, Karande AA, Rajasekharan R (2001) Isolation and localization of a cytosolic 10 S triacylglycerol biosynthetic multienzyme complex from oleaginous yeast. J Biol Chem 276: 10290-10298
7. Hobbs DH, Lu C, Hills MJ (1999) Cloning of cDNA encoding diacylglycerol acyltransfetase from Arabidopsis thaliana and its functional expression. FEBS Lett 452: 145-149
8. Ichihara K, Takahashi T, Fujii S (1988) Diacylglycerol acyltransferase in maturing safflower seeds: its influences on the fatty acid composition of triacylglycerol and on the rate of triacylglycerol synthesis. Biochim Biophys Acta 958: 125-129
9. Kalscheuer R, Steinbuchel A (2003) A novel bifunctional wax ester synthase/acyl-CoA: diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1. J Biol Chem 278: 8075-8082
10. Kennedy EP (1961) Biosynthesis of complex lipids. Fed Proc 20: 934-940
11. Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, Hawkins DJ (2001) DGAT2 is a new diacylglycerol acyltransferase gene family: purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity. J Biol Chem 276: 38862-38869
12. Murata N, Tasaka Y (1997) Glycerol-3-phosphate acyltransferase in plants. Biochim Biophys Acta 1348: 10-16
13. Murphy DJ (1988) A highly active soluble diacylglycerol synthesizing system from developing rapeseed, Brassica napus L. Lipids 23: 157-163
14. Nykiforuk CL, Furukawa-Stoffer TL, Huff PW, Sarna M, Laroche A, Moloney MM, Weselake RJ (2002) Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose mediated induction of enzyme biosynthesis. Biochim Biophys Acta 1580: 95-109
15. Oelkers P, Tinkelenberg A, Erdeniz N, Cromley D, Billheimer JT, Sturley SL (2000) A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast. J Biol Chem 275: 15609-15612
16. Ohlrogge JB, Browse J, Somerville C (1991) The genetics of plant lipids. Biochim Biophys Acta 1082: 1-26
17. Perriere G, Gouy M (1996) WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78: 364-369
18. Rudrabhatla P, Rajasekharan R (2002) Developmentally regulated dual-specificity kinase from peanut that is induced by abiotic stresses. Plant Physiol 130: 380-390
19. Shekar S, Tumaney AW, Rao TJVS, Rajasekharan R (2002) Isolation of lysophosphatidic acid phosphatase from developing peanut cotyledons. Plant Physiol 128: 988-996
20. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olsen BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85
21. Stobart K, Mancha M, Lenman M, Dahlqvist A, Stymne S (1997) Triacylglycerols are synthesized and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctorius L.) seeds. Planta 203: 58-66
22. Tumaney AW, Rajasekharan R (1999) Synthesis of azidophospholipids and labeling of lysophosphatidylcholine acyltransferase from developing soybean cotyledons. Biochim Biophys Acta 1439: 47-56
23. Tumaney AW, Shekar S, Rajasekharan R (2001) Identification, purification, and characterization of monoacylglycerol acyltransferase from developing peanut cotyledons. J Biol Chem 276: 10847-10852
24. Uthoff S, Stoveken T, Weber N, Vosmann K, Klein E, Kalscheuer R, Steinbuchel A (2005) Thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase of Acinetobacter sp. strain ADP1. Appl Environ Microbiol 71: 790-796
25. Yamada K, Lim J, Dale JM, Chen H, Shinn P, Palm CJ, Southwick AM, Wu HC, Kim C, Nguyen M, et al (2003) Empirical analysis of transcriptional activity in the Arabidopsis genome. Science 302: 842-846
26. Yen CL, Monetti M, Burri BJ, Farese RV Jr (2005) The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters. J Lipid Res 46: 1502-1511
27. Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W (2004) GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol 136: 2621-2632
28. Zou J, Wei Y, Jako C, Kumar A, Selvaraj G, Taylor DC (1999) The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19: 645-653