DGAT2 is a new diacylglycerol acyltransferase gene family. Purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity

Journal of Biological Chemistry

Volumn 276, Issue 42, 2001, Pages 38862-38869

  Lardizabal, Kathryn D ^a   Mai, Jennifer T ^b   Wagner, Nicholas W ^b   Wyrick, Annette ^b   Voelker, Toni ^b   Hawkins, Deborah J ^b  

^a Calgene LLC Monsanto   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CELLS; ENZYMES; FUNGI; LIPIDS; POLYPEPTIDES;

HOMOLOGY;

GENES;

CHOLESTEROL ACYLTRANSFERASE; DIACYLGLYCEROL ACYLTRANSFERASE; HEPARIN; HYDROXYAPATITE; POLYPEPTIDE; PROTEIN DGAT2; TRIACYLGLYCEROL; UNCLASSIFIED DRUG; ACYLTRANSFERASE; COMPLEMENTARY DNA;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; DNA SEQUENCE; ENZYME MECHANISM; GENE ACTIVITY; GENE EXPRESSION; INSECT CELL; MOLECULAR CLONING; MOLECULAR WEIGHT; MORTIERELLA RAMANNIANA; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; ANIMAL; CELL LINE; CHEMISTRY; CLASSIFICATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; INSECT; METABOLISM; MOLECULAR GENETICS; MORTIERELLA; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE;

ANIMALIA; FUNGI; INSECTA; MORTIERELLA; UMBELOPSIS RAMANNIANA;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CHROMATOGRAPHY; CLONING, MOLECULAR; DIACYLGLYCEROL O-ACYLTRANSFERASE; DNA, COMPLEMENTARY; DURAPATITE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEPARIN; HYDROGEN-ION CONCENTRATION; INSECTS; MOLECULAR SEQUENCE DATA; MORTIERELLA; MULTIGENE FAMILY; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTEIN BINDING; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0035914412     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M106168200     Document Type: Article

References (35)

1. Harwood, J. (1996) Biochim. Biophys. Acta 1301, 7-56
2. Daum, G., Lees, N. D., Bard, M., and Dickson, R. (1998) Yeast 16, 1471-1510
3. Coleman, R., Lewin, T., and Muoio, D. (2000) Annu. Rev. Nutr. 20, 77-103
4. Bell, R. M., and Coleman, R. A. (1980) Annu. Rev. Biochem. 49, 459-487
5. Brindley, D. N. (1991) in Biochemistry of Lipids, Lipoproteins and Membranes (Vance, D. E. and Vance, J. E., eds) pp. 171-203, Elsevier Science Publishers B.V., Amsterdam
6. Nishizuka, Y. (1992) Science 258, 607-614
7. Cases, S., Smith, S. J., Zheng, Y.-W., Myers, H. M., Lear, S. R., Sande, E., Novak, S., Collins, C., Welch, C. B., Lusis, A. J., Erickson, S. K., and Farese, R. V. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 13018-13023
8. Hobbs, D. H., Lu, C., and Hills, M. J. (1999) FEBS Lett. 452, 145-149
9. Katavic, V., Reed, D. W., Taylor, D. C., Giblin, E. M., Barton, D. L., Zou, J.-T., MacKenzie, S. L., Covello, P. S., and Kunst, L. (1995) Plant Physiol. 108, 399-409
10. Zou, J., Wei, Y., Jako, C., Kumar, A., Selvaraj, G., and Taylor, D. C. (1999) Plant J. 19, 645-653
11. Routaboul, J.-M., Benning, C., Bechtold, N., Caboche, M., and Lepiniec, L. (1999) Plant Physiol. Biochem. 37, 831-840
12. Kamisaka, Y., Yokochi, T., Nakahara, T., and Suzuki, O. (1993) Lipids 28, 583-587
13. Kamisaka, Y., and Nakahara, T. (1996) J. Biochem. (Tokyo) 119, 520-523
14. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
15. Laemmli, U. K. (1970) Nature 227, 680-685
16. Delepelaire, P. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 111-115
17. Blum, H. (1987) Electrophoresis 8, 93-99
18. Jones, A., Davies, H. D., and Voelker, T. (1995) Plant Cell 7, 359-371
19. Kamisaka, Y., Mishra, S., and Nakahara, T. (1997) J. Biochem. (Tokyo) 121, 1107-1114
20. Neuwald, F. (1997) Curr. Biol. 7, 465-466
21. Lewin, T. M., Wang, P., and Coleman, R. A. (1999) Biochemistry 38, 5764-5771
22. Smith, S. J., Cases, S., Jensen, D. R., Chen, H. C., Sande, E., Tow, B., Sanan, D. A., Raber, J., Eckel, R. H., and Farese, R. V. (2000) Nat. Genet. 25, 87-90
23. Blum, H., Beier, H., and Gross, H. J. (1987) Electrophoresis 8, 93-99
24. Dahlqvist, A., Banas, A., and Stymne, S. (1998) in Advances in Plant Lipid Research (Sanches, J., Cerda-Olmedo, E., and Martinez-Force, E., eds) pp. 211-214, Universidad de Sevilla, Seville, Spain
25. Oelkers, P., Tinkelenberg, A., Erdeniz, N., Cromley, D., Billheimer, J. T., and Sturley, S. L. (2000) J. Biol. Chem. 275, 15609-15612
26. Dahlqvist, A., Stahl, U. Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H., and Stymne, S. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 6487-6492
27. Bouvier-Nave, P., Benveniste, P., Oelkers, P., Sturley, S. L., and Schaller, H. (2000) Eur. J. Biochem. 267, 85-96
28. Knutzon, D. S., Thompson, G. A., Radke, S. E., Johnson, W. B., Knauf, V. C., and Kridl, J. C. (1992) Proc. Nat. Acad. Sci. U. S. A. 89, 2624-2628
29. Voelker, T. A., Worrell, A. C., Anderson, L., Bleibaum, J., Fan, C., Hawkins, D., Radke, S., and Davies, H. M. (1992) Science 257, 72-74
30. Metz, J. G., Pollard, M. R., Anderson, L., Hayes, T. R., and Lassner, M. W. (2000) Plant Physiol. 122, 635-644
31. Lardizabal, K. D., Metz, J. G., Sakamoto, T., Hutton, W. C., Pollard, M. R., and Lassner, M. W. (2000) Plant Physiol. 122, 645-655
32. Roesler, K., Shintani, D., Savage, L., Boddupalli, S., and Ohlrogge, J. (1997) Plant Physiol. 113, 75-81
33. Zou, J., Taylor, D. C., Katavic, V., MacKenzie, S., and Keller, W. (April 18, 2000) United States Patent 6, 051, 755
34. Suzuki, S., Arai, M., Mural, N., Finnegan, P., and Burnell, J. (March 9, 1999) United States Patent 5, 880, 334
35. Cases, S., Stone, S., Zhou, P., Yen, E., Tow, B., Lardizabal, K. D., Voelker, T., and Farese, R. V., Jr. (2001) J. Biol. Chem. 276, 38870-38876