Human mesenchymal stem cells from chorionic villi and amniotic fluid are not susceptible to transformation after extensive in vitro expansion

Cell Transplantation

Volumn 20, Issue 5, 2011, Pages 643-654

  Poloni, Antonella ^a   Maurizi, Giulia ^a   Babini, Lucia ^a   Serrani, Federica ^a   Berardinelli, Eleonora ^a   Mancini, Stefania ^a   Costantini, Benedetta ^a   Discepoli, Giancarlo ^b   Leoni, Pietro ^a  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b OSPEDALI RIUNITI   (Italy)

Author keywords

Fetal mesenchymal stem cells (MSCs); Human telomerase reverse transcriptase (hTERT); Telomerase activity; Telomere

Indexed keywords

PROTEIN P53; TELOMERASE; TELOMERASE REVERSE TRANSCRIPTASE;

ADOLESCENT; ADULT; AMNION FLUID; ARTICLE; BONE MARROW; CELL ANCHORAGE; CELL DIFFERENTIATION; CELL EXPANSION; CELL GROWTH; CELL PROLIFERATION; CHORION VILLUS; CHROMOSOME; CYTOGENETICS; ENZYME ACTIVITY; FEMALE; FIRST TRIMESTER PREGNANCY; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HUMAN TISSUE; IMMUNOPHENOTYPING; IN VITRO STUDY; ISOLATION PROCEDURE; KARYOTYPE; MESENCHYMAL STEM CELL; NORMAL HUMAN; ONCOGENE C MYC; PRENATAL SCREENING; PRIORITY JOURNAL; PROTEIN EXPRESSION; SECOND TRIMESTER PREGNANCY; SENESCENCE; TELOMERE;

ADOLESCENT; ADULT; AMNIOTIC FLUID; CELL DIFFERENTIATION; CELLS, CULTURED; CHORIONIC VILLI; FEMALE; HUMANS; KARYOTYPING; MESENCHYMAL STEM CELLS; PREGNANCY; PROTO-ONCOGENE PROTEINS C-MYC; TELOMERASE; TELOMERE; TUMOR SUPPRESSOR PROTEIN P53;

EID: 79959945787     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/096368910X536518     Document Type: Article

References (31)

1. Avanzini, M. A.; Bernardo, M. E.; Cometa, A. M.; Perotti, C.; Zaffarono, N.; Novara, F.; Visai, L.; Moretta, A.; Del Fante, C.; Villa, R.; Ball, L. M.; Fibbe, W. E.; Maccario, R.; Locatelli, F. Generation of mesenchymal stem cell in the presence of platelet lysate: A phenotypical and func- tional comparison of umbilical cord bloodand bone mar- row-derived progenitors. Haematologica 94:1649-1660; 2009.
2. Baxter, M. A.; Wynn, R. F.; Jowitt, S. N.; Wraith, J.; Fair- bairn, L.; Bellantuono, I. Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells 22:675-682; 2004.
3. Bernardo, M. E.; Zaffaroni, N.; Novara, F.; Cometa, A. M.; Avanzino, M. A.; Moretta, A.; Montagna, D.; Maccario, R.; Villa, R.; Daidone, M. G.; Zuffardi, O.; Locatelli, F. Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long term in vitro culture and do not exhibit telomere maintenance mechanisms. Cancer Res. 67:9142-9149; 2007.
4. Blackburn, E. H. Switching and signaling at the telomere. Cell 106:661-673; 2001. 653
5. Bossolasco, P.; Montemurro, T.; Cova, L.; Zangrossi, S.; Calzarossa, C.; Buiatiotis, S.; Soligo, D.; Bosari, S.; Silani, V.; Deliliers, G. L.; Rebulla, P.; Lazzari, L. Molec- ular and phenotypic characterization of human amniotic fluid cells and their differentiation potential. Cell Res. 16: 329-336; 2006.
6. Cananzi, M.; Atala, A.; De Coppi, P. Stem cells derived from amniotic fluid: New potentials in regenerative medi- cine. Reprod. Biomed. Online 18:17-27; 2009.
7. De Pinho, R. A. The age of cancer. Nature 408:248-254; 2000.
8. Guillot, P. V.; Gotherstrom, C.; Chan, J.; Kurata, H.; Fisk, N. M. Human first trimester fetal MSC express pluripo- tency markers and grow faster and have longer telomeres than adult MSC. Stem Cells 25:646-654; 2007.
9. Henson, J. D.; Neumann, A. A.; Yeager, T. R.; Reddel, R. R. Alternative lengthening of telomeres in mammalian cells. Oncogene 21:598-610; 2002.
10. Hertzog, R. G. Ancestral telomere shortening: A count- down that will increase mean life span? Med. Hypotheses 67:157-160; 2006.
11. Hiyama, E.; Hiyama, K. Telomere and telomerase in stem cells. Br. J. Cancer 96:1020-1024; 2007.
12. Horikawa, I.; Barrett, J. C. Transcriptional regulation of the telomerase hTERT gene as a target for cellular and viral oncogenic mechanisms. Carcinogenesis 24:1167-1176; 2003.
13. Jo, C. H.; Kim, O.; Park, E.; Kim, B. J.; Lee, J. H.; Kang, S. B.; Lee, J. H.; Han, H. S.; Rhee, S. B.; Yoon, K. S. Fetal mesenchymal stem cells derived from human umbil- ical cord sustain primitive characteristics during extensive expansion. Cell Tissue Res. 334:423-433; 2008.
14. Markway, B. D.; Tan, G. K.; Brooke, G.; Hudson, J. E.; Cooper-White, J. J.; Doran, M. R. Enhanced chondrogenic differentiation of human bone marrow-derived mesenchy- mal stem cells in low oxygen environment micropellet cultures. Cell Transplant. 19:29-42; 2010.
15. Miura, M.; Miura, Y.; Padilla-Nash, H.; Molinolo, A. A.; Fu, B.; Patel, V.; Seo, B. M.; Sonoyama, W.; Zheng, J.; Baker, C. C.; Chen, W.; Ried, T.; Shi, S. Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation. Stem Cells 24:1095-1103; 2006.
16. Oishi, K.; Noguchi, H.; Yukawa, H.; Hayashi, S. Differential ability of somatic stem cells. Cell Transplant. 18:581- 589; 2009.
17. Pieternella, S.; Sherjon, S. A.; Fibbe, W. E. Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22:1338-1345; 2004.
18. Poloni, A.; Maurizi, G.; Rosini, V.; Mondini, E.; Mancini, S.; Discepoli, G.; Biasio, S.; Battaglini, G.; Felicetti, S.; Berardinelli, E.; Serrani, F.; Leoni, P. Selection of CD271+ cells and human AB serum allows a large expan- sion of mesenchymal stromal cells from human bone mar- row. Cytotherapy 11:153-162; 2009.
19. Poloni, A.; Rosini, V.; Mondini, E.; Maurizi, G.; Mancini, S.; Discepoli, G.; Biasio, S.; Battaglini, G.; Berardinelli, E.; Serrani, F.; Leoni, P. Characterization and expansion of mesenchymal progenitor cells from first-trimester chorionic villi of human placenta. Cytotherapy 10:690-697; 2008.
20. Rosland, G. V.; Svedsen, A.; Torsvik, A.; Sobala, E.; McCormack, E.; Immervoll, H.; Mysliwietz, J.; Tonn, J. C.; Goldbrunner, R.; Lonning, P. E.; Bjerkvig, R.; Schi- chor, C. Long-term cultures of bone marrow-derived hu- man mesenchymal stem cells frequently undergo spontane-654 ous malignant transformation. Cancer Res. 69:5331-5339; 2009.
21. Rubio, D.; Garcia-Castro, J.; Martin, M. C.; de la Fuente, R.; Cigudosa, J. C.; Lloyd, A. C.; Bernad, A. Spontaneous human adult stem cell transformation. Cancer Res. 65: 3035-3039; 2005.
22. Salvadè, A.; Della Mina, P.; Gaddi, D.; Gatto, M.; Villa, A.; Bigoni, M.; Perseghin, P.; Serafini, M.; Zatti, G.; Biondi, A.; Biagi, E. Characterization of platelet lysate cultured mesenchymal stromal cells and their potential use in tissue engineered osteogenic devices for the treatment of bone defects. Tissue Eng. Part C Methods 16:201-214; 2010.
23. Serakinci, N.; Hoare, S. F.; Kassem, M.; Atkinson, S. P.; Keith, W. N. Telomerase promoter reprogramming and in- teraction with general transcription factors in the human mesenchymal stem cell. Regen. Med. 1:125-131; 2006.
24. Serakinci, N.; Hoare, S. F.; Kassem, M.; Atkinson, S. P.; Keith, W. N. Telomere stability and telomerase in mesen- chymal stem cells. Biochimie 90:33-40; 2008.
25. Shaffer, L. G.; Tommerup, N. An international system for human cytogenetic nomenclature. Basel, Switzerland: S. Karger A.G.; 2005.
26. Staples, O. D.; Steele, R. J.; Lain, S. p53 as a therapeutic target. Surgeon 6:240-243; 2008. POLONI ET AL.
27. Tarte, K.; Gaillard, J.; Lataillade, J.; Fouillard, L.; Becker, M.; Mossafa, H.; Tchirkov, A.; Rouard, H.; Henry, C.; Splingard, M.; Dulong, J.; Monnier, D.; Gourmelon, P.; Gorin, N. C.; Sensebe, L. Clinical-grade production of hu- man mesenchymal stromal cells: Occurrence of aneuplody without transformation. Blood 25:1549-1553; 2010.
28. Terrin, L.; Dolcetti, R.; Corradini, I.; Indraccolo, S.; Dal Col, J.; Bertorelli, R.; Bonaldi, L.; Esposito, G.; De Rossi, A. hTERT inhibits Epstein-Barr virus lytic cycle and pro- motes the proliferation of primary B lynphocytes: Implica- tions for EBV driven lymphomagenesis. Int. J. Cancer 121:576-587; 2007.
29. Wang, Y.; Huso, D. L.; Harrington, J.; Kellner, J.; Jeong, D. K.; Turney, J.; McNiece, I. K. Outgrowth of a trans- formed cell population derived from normal human BM mesenchymal stem cell culture. Cytotherapy 7:509-519; 2005.
30. Wright, W. E.; Shay, J. W. Historical claims and current interpretations of replicative aging. Nat. Biotechnol. 20: 682-688; 2002.
31. Zhao, Y.; Li, J.; Lan, J.; Xiao-Yu, L.; Yi, L.; Jie, S.; Jian, Y.; Yuan-Yuan, Z.; Fen- Fang, Z.; Quian, Z.; He, H. Cell cycle telomere regulation by telomerase in human bone marrow mesenchymal stem cells. Biochem. Biophys. Res. Commun. 369:1114-1119; 2008.