Myosin heavy chain expression pattern as a marker for anabolic potency: Desoxymethyltestosterone (madol), norandrostendione and testosterone repress MHC-IIb expression and stimulate MHC-IId/x expression in orchiectomized rat gastrocnemius muscle

Archives of Toxicology

Volumn 85, Issue 6, 2011, Pages 635-643

  Frese, S ^a   Velders, M ^a   Schleipen, B ^a   Schanzer W ^a   Bloch, W ^a   Diel, P ^a  

^a GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

Author keywords

Desoxymethyltestosterone; Fiber type composition; Madol; Myosin heavy chain; NORandrostendione; Skeletal muscle; Testosterone

Indexed keywords

DESOXYMETHYLTESTOSTERONE; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2A; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2B; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2D/X; MYOSIN ADENOSINE TRIPHOSPHATASE; MYOSIN HEAVY CHAIN; NORANDROSTENDIONE; STEROID HORMONE; TESTOSTERONE PROPIONATE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; DRUG EFFECT; DRUG POTENCY; GASTROCNEMIUS MUSCLE; HEART WEIGHT; LEVATOR ANI MUSCLE; LIVER WEIGHT; MALE; MUSCLE CELL; MUSCLE GROWTH; NONHUMAN; ORCHIECTOMY; PRIORITY JOURNAL; PROSTATE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RAT; SEMINAL VESICLE; UPREGULATION; WESTERN BLOTTING;

ANABOLIC AGENTS; ANDROGENS; ANDROSTENEDIONE; ANDROSTENOLS; ANIMALS; BIOLOGICAL MARKERS; DESIGNER DRUGS; DOWN-REGULATION; HINDLIMB; MALE; MOLECULAR WEIGHT; MUSCLE FIBERS, SKELETAL; MUSCLE, SKELETAL; MYOSIN HEAVY CHAINS; ORCHIECTOMY; ORGAN SIZE; PROTEIN ISOFORMS; RANDOM ALLOCATION; RATS; RATS, WISTAR; TESTOSTERONE PROPIONATE; UP-REGULATION;

RATTUS; RATTUS NORVEGICUS;

EID: 79959620111     PISSN: 03405761     EISSN: 14320738     Source Type: Journal    
DOI: 10.1007/s00204-010-0607-8     Document Type: Article

References (51)

1. Adams GR, Hather BM, Baldwin KM, Dudley GA (1993) Skeletal muscle myosin heavy chain composition and resistance training. J Appl Physiol Respir Environ Exerc Physiol 74(2):911-915 (Pubitemid 23078194)
2. Bhasin S, Storer TW, Berman N, Callegari C, Clevenger B, Phillips J, Bunnell TJ, Tricker R, Shirazi A, Casaburi R (1996) The effects of supraphysiologic doses of testosterone on muscle size and strength in normal men. N Engl J Med 335(1):1-7 (Pubitemid 26230806)
3. Boada LD, Zumbado M, Torres S, Lopez A, Diaz-Chico BN, Cabrera JJ, Luzardo OP (1999) Evaluation of acute and chronic hepatotoxic effects exerted by anabolic-androgenic steroid stanozolol in adult male rats. Arch Toxicol 73(8-9):465-472 (Pubitemid 30010248)
4. Brooke MH, Kaiser KK (1970) Three "myosin adenosine triphosphatase" systems: the nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem 18(9):670-672
5. Catlin DH, Sekera MH, Ahrens BD, Starcevic B, Chang YC, Hatton CK (2004) Tetrahydrogestrinone: discovery, synthesis, and detection in urine. Rapid Commun Mass Spectrom 18(12):1245-1249
6. D'Antona G, Lanfranconi F, Pellegrino MA, Brocca L, Adami R, Rossi R, Moro G, Miotti D, Canepari M, Bottinelli R (2006) Skeletal muscle hypertrophy and structure and function of skeletal muscle fibres in male body builders. J Physiol 570(Pt 3):611-627
7. Delp MD, Duan C (1996) Composition and size of type i, iia, iid/x, and iib fibers and citrate synthase activity of rat muscle. J Appl Physiol 80(1):261-270 (Pubitemid 26034871)
8. Dickerman RD, Schaller F, Zachariah NY, McConathy WJ (1997) Left ventricular size and function in elite bodybuilders using anabolic steroids. Clin J Sport Med 7(2):90-93 (Pubitemid 27202144)
9. Dickerman RD, Schaller F, McConathy WJ (1998) Left ventricular wall thickening does occur in elite power athletes with or without anabolic steroid use. Cardiology 90(2):145-148 (Pubitemid 28486692)
10. Diel P, Friedel A, Geyer H, Kamber M, Laudenbach-Leschowsky U, Schanzer W, Thevis M, Vollmer G, Zierau O (2007) Characterisation of the pharmacological profile of desoxymethyltestosterone (madol), a steroid misused for doping. Toxicol Lett 169(1):64-71 (Pubitemid 46215956)
11. Diel P, Baadners D, Schlupmann K, Velders M, Schwarz JP (2008a) C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and pax7 expression. J Mol Endocrinol 40(5):231-241 (Pubitemid 351865031)
12. Diel P, Friedel A, Geyer H, Kamber M, Laudenbach-Leschowsky U, Schanzer W, Schleipen B, Thevis M, Vollmer G, Zierau O (2008b) The prohormone 19-norandrostenedione displays selective androgen receptor modulator (sarm) like properties after subcutaneous administration. Toxicol Lett 177(3):198-204
13. Dorn SB, Bolt HM, Thevis M, Diel P, Degen GH (2008a) Induction of micronuclei in v79 cells by the anabolic doping steroids tetrahydrogestrinone and trenbolone. Arch Toxicol 82(4):257-263 (Pubitemid 351661649)
14. Dorn SB, Bolt HM, Thevis M, Diel P, Degen GH (2008b) Micronucleus induction in v79 cells by the anabolic doping steroids desoxymethyltestosterone (madol) and 19-norandrostenedione. Toxicol Lett 183(1-3):58-64
15. Eisenberg E, Gordan GS (1950) The levator ani muscle of the rat as an index of myotrophic activity of steroidal hormones. J Pharmacol Exp Ther 99(1):38-44
16. Eriksson A, Kadi F, Malm C, Thornell LE (2005) Skeletal muscle morphology in power-lifters with and without anabolic steroids. Histochem Cell Biol 124(2):167-175 (Pubitemid 41429772)
17. Ferrando AA, Sheffield-Moore M, Yeckel CW, Gilkison C, Jiang J, Achacosa A, Lieberman SA, Tipton K, Wolfe RR, Urban RJ (2002) Testosterone administration to older men improves muscle function: molecular and physiological mechanisms. Am J Physiol 282(3):E601-E607 (Pubitemid 34654776)
18. Ferry A, Noirez P, Page CL, Salah IB, Daegelen D, Rieu M (1999) Effects of anabolic/androgenic steroids on regenerating skeletal muscles in the rat. Acta Physiol Scand 166(2):105-110 (Pubitemid 29304169)
19. Friedel A, Geyer H, Kamber M, Laudenbach-Leschowsky U, Schanzer W, Thevis M, Vollmer G, Zierau O, Diel P (2006) Tetrahydrogestrinone is a potent but unselective binding steroid and affects glucocorticoid signalling in the liver. Toxicol Lett 164(1):16-23
20. Harjola V, Jankala H, Harkonen M (2000) Myosin heavy chain mRNA and protein distribution in immobilized rat skeletal muscle are not affected by testosterone status. Acta Physiol Scand 169(4):277-282
21. Hartgens F, Kuipers H, Wijnen JA, Keizer HA (1996) Body composition, cardiovascular risk factors and liver function in long-term androgenic-anabolic steroids using bodybuilders three months after drug withdrawal. Int J Sports Med 17(6):429-433 (Pubitemid 26317137)
22. Hartgens F, van Straaten H, Fideldij S, Rietjens G, Keizer HA, Kuipers H (2002) Misuse of androgenic-anabolic steroids and human deltoid muscle fibers: differences between polydrug regimens and single drug administration. Eur J Appl Physiol 86(3):233-239 (Pubitemid 36067476)
23. Hartsook EW, Hershberger TV (1961) Effect of synthetic steroid or phenanthrene compounds upon weight gain and feed efficiency of rats. Proc Soc Exp Biol Med Soc Exp Biol Med 106:57-60
24. Hather BM, Tesch PA, Buchanan P, Dudley GA (1991) Influence of eccentric actions on skeletal muscle adaptations to resistance training. Acta Physiol Scand 143(2):177-185
25. Hershberger LG, Shipley EG, Meyer RK (1953) Myotrophic activity of 19-nortestosterone and other steroids determined by modified levator ani muscle method. Proc Soc Exp Biol Med Soc Exp Biol Med (New York, 83(1): 175-180
26. Hobbs CJ, Plymate SR, Rosen CJ, Adler RA (1993) Testosterone administration increases insulin-like growth factor-i levels in normal men. J Clin Endocrinol Metab 77(3):776-779 (Pubitemid 23298665)
27. Johansen JA, Breedlove SM, Jordan CL (2007) Androgen receptor expression in the levator ani muscle of male mice. J Neuroendocrinol 19(10):823-826 (Pubitemid 47386884)
28. Joumaa WH, Serrurier B, Bigard X, Leoty C (2002) Nandrolone decanoate treatment induces changes in contractile responses of rat untrained fast-twitch skeletal muscle. Acta Physiol Scand 175(3):189-199 (Pubitemid 34779422)
29. Kadi F, Eriksson A, Holmner S, Thornell LE (1999) Effects of anabolic steroids on the muscle cells of strength-trained athletes. Med Sci Sports Exerc 31(11):1528-1534
30. Kamanga-Sollo E, White ME, Hathaway MR, Chung KY, Johnson BJ, Dayton WR (2008) Roles of igf-i and the estrogen, androgen and igf-i receptors in estradiol-17beta- and trenbolone acetate-stimulated proliferation of cultured bovine satellite cells. Domest Anim Endocrinol 35(1):88-97
31. Kazlauskas R (2004) Designer steroids. Handb Exp Pharmacol 195:155-185
32. Kicl FD, Dorfman RI (1964) Anabolic-androgenic potency of various steroids in a castrated rat assay. Steroids 3(1):109-122
33. Maravelias C, Dona A, Stefanidou M, Spiliopoulou C (2005) Adverse effects of anabolic steroids in athletes. A constant threat. Toxicol Lett 158(3):167-175 (Pubitemid 41074813)
34. Marks LS (2004) 5alpha-reductase: history and clinical importance. Rev Urol 6(Suppl 9):S11-S21
35. McGinnis MY, Lumia AR, Possidente BP (2002) Effects of withdrawal from anabolic androgenic steroids on aggression in adult male rats. Physiol Behav 75(4):541-549 (Pubitemid 34327961)
36. Miyazaki M, Hitomi Y, Kizaki T, Ohno H, Haga S, Takemasa T (2004) Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition. J Physiol Pharmacol 55(4):751-764 (Pubitemid 40053043)
37. Noirez P, Ferry A (2000) Effect of anabolic/androgenic steroids on myosin heavy chain expression in hindlimb muscles of male rats. Eur J Appl Physiol 81(1-2):155-158 (Pubitemid 30008873)
38. Parr MK, Laudenbach-Leschowsky U, Hofer N, Schanzer W, Diel P (2009) Anabolic and androgenic activity of 19-norandrostenedione after oral and subcutaneous administration-analysis of side effects and metabolism. Toxicol Lett 188(2):137-141
39. Pehme A, Alev K, Kaasik P, Julkunen A, Seene T (2004) The effect of mechanical loading on the myhc synthesis rate and composition in rat plantaris muscle. Int J Sports Med 25(5):332-338 (Pubitemid 38916409)
40. Pette D, Staron RS (2000) Myosin isoforms, muscle fiber types, and transitions. Microsc Res Tech 50(6):500-509
41. Rogerson S, Weatherby RP, Deakin GB, Meir RA, Coutts RA, Zhou S, Marshall-Gradisnik SM (2007) The effect of short-term use of testosterone enanthate on muscular strength and power in healthy young men. J Strength Cond Res 21(2):354-361
42. Roy RR, Talmadge RJ, Fox K, Lee M, Ishihara A, Edgerton VR (1997) Modulation of mhc isoforms in functionally overloaded and exercised rat plantaris fibers. J Appl Physiol: Respir, Environ Exerc Physiol 83(1):280-290 (Pubitemid 27304760)
43. Russell DW, Wilson JD (1994) Steroid 5 alpha-reductase: two genes/two enzymes. Annu Rev Biochem 63:25-61
44. Scott W, Stevens J, Binder-Macleod SA (2001) Human skeletal muscle fiber type classifications. Phys Ther 81(11):1810-1816 (Pubitemid 33027477)
45. Sekera MH, Ahrens BD, Chang YC, Starcevic B, Georgakopoulos C, Catlin DH (2005) Another designer steroid: discovery, synthesis, and detection of 'madol' in urine. Rapid Commun Mass Spectrom 19(6):781-784
46. Shin JH, Moon HJ, Kang IH, Kim TS, Lee SJ, Ahn JY, Bae H, Jeung EB, Han SY (2007) Oecd validation of the rodent hershberger assay using three reference chemicals; 17alpha-methyltestosterone, procymidone, and p, p′-dde. Arch Toxicol 81(5):309-318 (Pubitemid 46701596)
47. Singh R, Artaza JN, Taylor WE, Gonzalez-Cadavid NF, Bhasin S (2003) Androgens stimulate myogenic differentiation and inhibit adipogenesis in c3 h 10t1/2 pluripotent cells through an androgen receptor-mediated pathway. Endocrinology 144(11):5081-5088 (Pubitemid 37378423)
48. Socas L, Zumbado M, Perez-Luzardo O, Ramos A, Perez C, Hernandez JR, Boada LD (2005) Hepatocellular adenomas associated with anabolic androgenic steroid abuse in bodybuilders: a report of two cases and a review of the literature. Br J Sports Med 39(5):e27
49. Steers WD (2001) 5alpha-reductase activity in the prostate. Urology 58(6 Suppl 1): 17-24; discussion 24
50. Tamaki T, Uchiyama S, Uchiyama Y, Akatsuka A, Roy RR, Edgerton VR (2001) Anabolic steroids increase exercise tolerance. Am J Physiol 280(6):E973-E981
51. Yamasaki K, Sawaki M, Ohta R, Okuda H, Katayama S, Yamada T, Ohta T, Kosaka T, Owens W (2003) Oecd validation of the hershberger assay in japan: phase 2 dose response of methyltestosterone, vinclozolin, and p,p′-dde. Environ health perspect 111(16):1912-1919 (Pubitemid 37541203)