Comparison of ceramic hydroxy- and fluoroapatite versus Protein A/G-based resins in the isolation of a recombinant human antibody from cell culture supernatant

Journal of Chromatography A

Volumn 1142, Issue 1 SPEC. ISS., 2007, Pages 106-113

  Schubert, Sven ^a   Freitag, Ruth ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Bioseparation; Comparison studies; Fluoroapatite; Hydroxyapatite; Monoclonal antibodies; Protein A

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; HYDROXYAPATITE; IMPURITIES; MONOCLONAL ANTIBODIES; PH EFFECTS; RESINS;

BIOSEPARATION; CHINESE HAMSTER OVARY (CHO); COMPARISON STUDIES; FLUOROAPATITE; PROTEIN A;

CELL CULTURE;

ANTIBODY; ARGININE; FLUORAPATITE; HYDROXYAPATITE; LIGAND; PHOSPHATE; PROTEIN A; PROTEIN G; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CERAMICS; CONTROLLED STUDY; FEMALE; FLOW KINETICS; FRACTIONATION; ISOELECTRIC POINT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN PURIFICATION; SUPERNATANT;

CRICETULUS GRISEUS;

EID: 33846607241     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2006.08.075     Document Type: Article

References (28)

1. Reichert J.M., Rosenweig C.J., Faden L.B., and Dewitz M.C. Nat. Biotechnol. 23 (2005) 1073
2. Vandevyver C., and Freitag R. In: Subramanian G. (Ed). Antibodies: Production and Purification Volume 1 (2004), Kluwer Academic/Plenum Publishers, New York, Boston, Dordrecht, London, Moscow 133 Chapter 5
3. Forsgren A., and Sjoquist J. J. Immunol. 97 (1966) 822
4. Eklund M., Axelsson L., Uhlen M., and Nygren P. Proteins 48 (2002) 454
5. Engel H., Mottl H., and Keck W. Protein Expr. Purif. 3 (1992) 108
6. Linhult M., Gulich S., Graslund T., Simon A., Karlsson M., Sjoberg A., Nord K., and Hober S. Proteins 55 (2004) 407
7. Raeder R., and Boyle M.D. Clin. Diagn. Lab. Immunol. 2 (1995) 484
8. Akerstrom B., and Bjorck L. J. Biol. Chem. 264 (1989) 19740
9. Fassina G., Verdoliva A., Odierna M.R., Ruvo M., and Cassini G. J. Mol. Recognit. 9 (1996) 564
10. Kihira Y., and Aiba S. J. Chromatogr. 597 (1992) 277
11. Stanker L.H., Vanderlaan M., and Juarez-Salinas H. J. Immunol. Methods 76 (1985) 157
12. Jungbauer A., Tauer C., Reiter M., Purtscher M., Wenisch E., Steindl F., Buchacher A., and Katinger H. J. Chromatogr. 476 (1989) 257
13. Giovannini R., and Freitag R. Biotechnol. Bioeng. 73 (2001) 522
14. Tiselius A., Hertjen S., and Levin O. Arch. Biochem. Biophys. 65 (1956) 132
15. Kawasaki T. J. Chromatogr. 544 (1991) 147
16. Gorbunoff M.J., and Timasheff S.N. Anal. Biochem. 136 (1984) 440
17. Kawasaki T., Niikura M., and Kobayashi Y. J. Chromatogr. 515 (1990) 125
18. Deppert W.R., and Lukacin R. In: Kastner M. (Ed). Protein Liquid Chromatography (2000), Elsevier, Amsterdam 271
19. Gorbunoff M. Methods Enzymol. 11 (1985) 370
20. Kawasaki T., Ikeda K., Takahashi S., and Kuboki Y. Eur. J. Biochem. 155 (1986) 249
21. Sato T., Su T., Ogawa T., and Okuyama T. Chromatogr. 19 (1998) 191
22. Miescher S., Zahn-Zabal M., de Jesus M., Moudry R., Fisch I., Vogel M., Korb M., Imboden M.A., Kragten E., Bichler J., Mermod N., Stadler B.M., Amstutz H., and Wurm F. Br. J. Haematol. 111 (2000) 157
23. Laemmli U.K. Nature 227 (1970) 680
24. Jordan M., Fraboulet D., Fourmestraux G., Wurm F., and Freitag R. In: Bernard A., Griffiths B., Noé W., and Wurm F. (Eds). Animal Cell Technology: Products from Cells, Cells as Products, Proceedings of the 16th ESACT conference (1999), Kluwer Academic Publishers 483
25. Amstutz H., Miescher S., Moudry R., Dejardin S., Imboden M., Morgenthaler J.J., Zahn M., Kobr M., Fisch I., Corthésy B., Mermod N., Berruex L., Abdelali M., Brüggemann O., Fraboulet D., Freitag R., Kragten E., Nahrgang S., Ducommun P., Pugeaud P., Marison I., von Stockar U., de Jesus M., Jordan M., Bourgeois M., Hunt L., Radice S., Baldi L., Kulangara A., Girard P., Ribicki R., and Wurm F. In: Bernard A., Griffiths B., Noé W., and Wurm F. (Eds). Animal Cell Technology: Products from Cells, Cells as Products, Proceedings of the 16th ESACT conference (1999), Kluwer Academic Publishers 455
26. von Stockar U., Kragten E., Marison I.W., Amstutz H., Mischer S., Fish I., Mermod N., de Jesus M., Freitag R., and Wurm F. Chimia 53 (1999) 554
27. Ejima D., Yumioka R., Tsumoto K., and Arakawa T. Anal. Biochem. 345 (2005) 250
28. Giovannini R., and Freitag R. Bioseparation 9 (2001) 359