The effect of Huwentoxin-I on Ca2+ channels in differentiated NG108-15 cells, a patch-clamp study

Toxicon

Volumn 39, Issue 4, 2001, Pages 491-498

  Peng, Kuan ^a   Chen, Xiang Ding ^a   Liang, Song Ping ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Neurotoxin; NG108 15 cell; Presynaptic inhibitor; Selenocosmia huwena; Voltage gated Ca2+ channels; Whole cell patch clamp

Indexed keywords

CALCIUM CHANNEL; HUWENTOXIN 1; NEUROTOXIN; NIFEDIPINE; OMEGA CONOTOXIN GVIA; PROSTAGLANDIN E1; SPIDER VENOM; TETRODOTOXIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CONTROLLED STUDY; ELECTRIC POTENTIAL; NONHUMAN; PATCH CLAMP; POTASSIUM CURRENT; PRESYNAPTIC INHIBITION; PRIORITY JOURNAL; SELENOCOSMIA HUWENA; SODIUM CURRENT; SPIDER;

EID: 0142042688     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(00)00150-1     Document Type: Article

References (18)

1. Basus V.J., Nadasdi L., Ramaohandran J., Miljanich G.P. Solution structure of ω -conotoxin MVIIA using 2D-NMR spectrescopy. FEBS Lett. 370:1995;163-169.
2. Byerly L., Yazejian B. Intracellular factors for the maintenance of calcium currents in perfused neurons from the snail, Lymnaea Stagnalis. J. Physiol. (London). 370:1986;631-650.
3. Harald Reuter. Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature. 301:1983;569-574.
4. 2+ channels in evoked release of noreponephrine from sympathetic neurons. Science. 239:1988;57-61.
5. Kasai H., Neher E. Dihydropyridine-sensitive and ω-conotoxin-sensitive calcium channels in a mammalian neuroblastoma-glioma cell line. J. physiol. (London). 488:1992;161-188.
6. Liang S.P., Chen X.D., Shu Q., Zhang Y.Q., Peng K. The presynaptic activity of Huwentoxin-I, a neurotoxin from the venom of the spider Selenocosmia huwena. Toxicon. 38:(9):2000;1237-1246.
7. Liang S.P., Zhang D.Y., Pan X., Zhou P.A. Properties and amino sequence of Huwentoxin-I, a neurotoxin purified from the venom of the Chinese bird spider Selenocosmia Huwena. Toxicon. 31:1993;969-978.
8. Liang S.P., Xia Z.X., Xie J.Y. Solid-phase synthesis of huwentoxin-I and its structure and bioactivity analysis. Science in China(C). 40:1997;449-457.
9. Lin J.W., Rudy B., Llinas R. Funnel-web spider venom and a toxin fraction block calcium current expressed from rat brain mRNA in Xenopus oocytes. Proc. Natl. Acad. Sci. USA. 87:1990;4538-4542.
10. Mccleskey E.W., Fox A.P., Feldman D.H., Crux L.J., Olivera B.M., Tsien R.W., Yoshikami D. ω-Conotoxin: Direct and persistent blockade of specific types of calcium channels in neurons but not muscle. Proc. Natl. Acad. Sci. USA. 84:1987;4327-4331.
11. Miller R.J. Multiple calcium channels and neuronal function. Science. 235:1987;46-52.
12. 2+ channels. J. Biol. Chem. 267:1992;1403-1406.
13. 2+ channels by the spider venom toxin ω -Aga-IIIA. Proc. Natl. Acad. Sci. USA. 88:1991;6628-6631.
14. Nowycky M.C., Fox A.P., Tsien R.W. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 316:1985;440-443.
15. Qu Y.X., Liang S.P., Ding J.Z., Liu X.C., Zhang R.J., Gu X.C. Proton nuclear magnetic resonance studies on Huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimentional structure in solution. J. Prot. Chem. 16:1997;565-574.
16. Watermam S.A. Role of N-, P- and Q-type voltage-gated calcium channels in transmitter release from sympathetic neurons in the mouse isolated vas deferens. Br. J. Phamacol. 12:1997;393-398.
17. Lukyanetz E.A. Diversity and properties of calcium channel types in NG 108-15 hybrid cells. Neuroscience. 87:1998;265-274.
18. Zhou P.A., Xie X.J., Li M., Yang D.M., Xie Z.P., Zong X., Liang S.P. Blockade of neuromuscular transmission by huwentoxin-I, purified from the venom of the Chinese bird spider Selenocosmia huwena. Toxicon. 35:1997;39-45.