Diagnostic accuracy of ultrasensitive heat-denatured HIV-1 p24 antigen in non-B subtypes in Kampala, Uganda

International Journal of STD and AIDS

Volumn 22, Issue 6, 2011, Pages 310-314

  Spacek, L A ^a   Lutwama, F ^b   Shihab, H M ^a   Summerton, J ^a   Kamya, M R ^c   Ronald, A ^c,d   Laeyendecker, O ^a,e   Quinn, T C ^a,e   Mayanja Kizza, H ^b  

^a DEPARTMENT OF PATHOLOGY   (United States)

^b Academic Alliance for AIDS Care and Prevention and the Infectious Diseases Institute   (Uganda)

^c MAKERERE UNIVERSITY COLLEGE OF HEALTH SCIENCES   (Uganda)

^d UNIVERSITY OF MANITOBA   (Canada)

^e NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Africa; Diagnosis; HIV; Monitoring ART; Non B subtypes; Resource limited setting; Sensitivity; Specificity; Uganda; Ultrasensitive HIV 1 p24 assay; Viral load

Indexed keywords

ANTIGEN P24;

CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; IMMUNOASSAY; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PREDICTIVE VALUE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; SENSITIVITY AND SPECIFICITY; UGANDA; ULTRASENSITIVE HIV 1 P24 ASSAY; VIRUS LOAD;

ADULT; DEVELOPING COUNTRIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV CORE PROTEIN P24; HIV INFECTIONS; HIV-1; HUMANS; LINEAR MODELS; PROTEIN DENATURATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UGANDA; VIRAL LOAD;

EID: 79959530384     PISSN: 09564624     EISSN: None     Source Type: Journal    
DOI: 10.1258/ijsa.2009.009363     Document Type: Review

References (33)

1. Hammer SM, Eron JJ Jr, Reiss P, et al. Antiretroviral treatment of adult HIV infection: 2008 recommendations of the International AIDS Society, USA panel. JAMA 2008;300:555-70
2. Fiscus SA, Cheng B, Crowe SM, et al. HIV-1 viral load assays for resource limited settings. PLoS Med 2006;3:e417
3. Schupbach J, Tomasik Z, Knuchel M, et al. Optimized virus disruption improves detection of HIV-1 p24 in particles and uncovers a p24 reactivity in patients with undetectable HIV-1 RNA under long-term HAART. J Med Virol 2006;78:1003-10
4. Ledergerber B, Flepp M, Boni J, et al. Human immunodeficiency virus type 1 p24 concentration measured by boosted ELISA of heat-denatured plasma correlates with decline in CD4 cells, progression to AIDS, and survival: comparison with viral RNA measurement. J Infect Dis 2000;181:1280-8
5. Sterling TR, Hoover DR, Astemborski J, et al. Heat-denatured human immunodeficiency virus type 1 protein 24 antigen: prognostic value in adults with early-stage disease. J Infect Dis 2002;186:1181-5
6. Boni J, Opravil M, Tomasik Z, et al. Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma. AIDS 1997;11:F47-F52
7. Respess RA, Cachafeiro A, Withum D, et al. Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings. J Clin Microbiol 2005;43:506-8
8. Schupbach J, Boni J, Flepp M, et al. Antiretroviral treatment monitoring with an improved HIV-1 p24 antigen test: an inexpensive alternative to tests for viral RNA. J Med Virol 2001;65:225-32
9. Schupbach J, Gunthard H, Joos B, et al. HIV-1 p24 may persist during long-term highly active antiretroviral therapy, increases little during short treatment breaks, and its rebound after treatment stop correlates with CD4+ T cell loss. J Acquir Immune Defic Syndr 2005;40:250-6
10. Erikstrup C, Kallestrup P, Zinyama-Gutsire RB, et al. p24 as a predictor of mortality in a cohort of HIV-1-infected adults in rural Africa. J Acquir Immune Defic Syndr 2008;48:345-9
11. Spacek LA, Henson C, Gray RH, et al. Evaluation of heat-denatured HIV-1 p24 antigen as an alternative, inexpensive marker for HIV-1 RNA viral load in non-B HIV-1 subtypes in sub-Saharan Africa. 10th Conference on Retroviruses and Opportunistic Infections. 2003
12. Stevens G, Rekhviashvili N, Scott LE, Gonin R, Stevens W. Evaluation of two commercially available, inexpensive alternative assays used for assessing viral load in a cohort of human immunodeficiency virus type 1 subtype C-infected patients from South Africa. J Clin Microbiol 2005;43:857-61
13. Pascual A, Cachafeiro A, Funk ML, Fiscus SA. Comparison of an assay using signal amplification of the heat-dissociated p24 antigen with the Roche Monitor human immunodeficiency virus RNA assay. J Clin Microbiol 2002;40:2472-5
14. Bonard D, Rouet F, Toni TA, et al. Field evaluation of an improved assay using a heat-dissociated p24 antigen for adults mainly infected with HIV-1 CRF02_AG strains in Cote d'Ivoire, West Africa. J Acquir Immune Defic Syndr 2003;34:267-73
15. Lombart JP, Vray M, Kafando A, et al. Plasma virion reverse transcriptase activity and heat dissociation-boosted p24 assay for HIV load in Burkina Faso, West Africa. AIDS 2005;19:1273-7
16. Ondoa P, Dieye TN, Vereecken C, et al. Evaluation of HIV-1 p24 antigenemia and level of CD8+ CD38+ T cells as surrogate markers of HIV-1 RNA viral load in HIV-1-infected patients in Dakar, Senegal. J Acquir Immune Defic Syndr 2006;41:416-24
17. Guay LA, Hom DL, Kabengera SR, et al. HIV-1 ICD p24 antigen detection in ugandan infants: use in early diagnosis of infection and as a marker of disease progression. J Med Virol 2000;62:426-34
18. Lyamuya E, Bredberg-Raden U, Massawe A, et al. Performance of a modified HIV-1 p24 antigen assay for early diagnosis of HIV-1 infection in infants and prediction of mother-to-infant transmission of HIV-1 in Dar es Salaam, Tanzania. J Acquir Immune Defic Syndr Hum Retrovirol 1996;12:421-6
19. De Baets AJ, Edidi BS, Kasali MJ, et al. Pediatric human immunodeficiency virus screening in an African district hospital. Clin Diagn Lab Immunol 2005;12:86-92
20. Fiscus SA, Pilcher CD, Miller WC, et al. Rapid, real-time detection of acute HIV infection in patients in Africa. J Infect Dis 2007;195:416-24
21. Herbeck JT, Lyagoba F, Moore SW, et al. Prevalence and genetic diversity of HIV type 1 subtypes A and D in women attending antenatal clinics in Uganda. AIDS Res Hum Retroviruses 2007;23:755-60
22. Kamya MR, Mayanja-Kizza H, Kambugu A, et al. Predictors of long-term viral failure among Ugandan children and adults treated with antiretroviral therapy. J Acquir Immune Defic Syndr 2007;46:187-93
23. Schockmel GA, Yerly S, Perrin L. Detection of low HIV-1 RNA levels in plasma. J Acquir Immune Defic Syndr Hum Retrovirol 1997;14:179-83
24. Schupbach J, Boni J, Bisset LR, et al. HIV-1 p24 antigen is a significant inverse correlate of CD4T-cell change in patients with suppressed viremia under long-term antiretroviral therapy. J Acquir Immune Defic Syndr 2003;33:292-9
25. Schupbach J, Flepp M, Pontelli D, Tomasik Z, Luthy R, Boni J. Heat-mediated immune complex dissociation and enzyme-linked immunosorbent assay signal amplification render p24 antigen detection in plasma as sensitive as HIV-1 RNA detection by polymerase chain reaction. AIDS 1996;10:1085-90
26. Ribas SG, Ondoa P, Schupbach J, van der GG, Fransen K. Performance of a quantitative human immunodeficiency virus type 1 p24 antigen assay on various HIV-1 subtypes for the follow-up of human immunodeficiency type 1 seropositive individuals. J Virol Methods 2003;113:29-34
27. Kim EY, Stanton J, Korber BT, et al. Detection of HIV-1 p24 Gag in plasma by a nanoparticle-based bio-barcode-amplification method. Nanomedicine 2008;3:293-303
28. Tang S, Zhao J, Storhoff JJ, et al. Nanoparticle-based biobarcode amplification assay (BCA) for sensitive and early detection of human immunodeficiency type 1 capsid (p24) antigen. J Acquir Immune Defic Syndr 2007;46:231-7
29. Jennings C, Fiscus SA, Crowe SM, et al. Comparison of two human immunodeficiency virus (HIV) RNA surrogate assays to the standard HIV RNA assay. J Clin Microbiol 2005;43:5950-6
30. Nadal D, Boni J, Kind C, et al. Prospective evaluation of amplification-boosted ELISA for heat-denatured p24 antigen for diagnosis and monitoring of pediatric human immunodeficiency virus type 1 infection. J Infect Dis 1999;180:1089-95
31. Schupbach J. Measurement of HIV-1 p24 antigen by signal-amplification-boosted ELISA of heat-denatured plasma is a simple and inexpensive alternative to tests for viral RNA. AIDS Rev 2002;4:83-92
32. Prado JG, Shintani A, Bofill M, Clotet B, Ruiz L, Martinez-Picado J. Lack of longitudinal intrapatient correlation between p24 antigenemia and levels of human immunodeficiency virus (HIV) type 1 RNA in patients with chronic HIV infection during structured treatment interruptions. J Clin Microbiol 2004;42:1620-5
33. Burgisser P, Vernazza P, Flepp M, et al. Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study. J Acquir Immune Defic Syndr 2000;23:138-44