Evaluation of two commercially available, inexpensive alternative assays used for assessing viral load in a cohort of human immunodeficiency virus type 1 subtype C-infected patients from South Africa

Journal of Clinical Microbiology

Volumn 43, Issue 2, 2005, Pages 857-861

  Stevens, G ^a   Rekhviashvili, N ^a   Scott, L E ^a   Gonin, René ^b   Stevens, W ^a  

^a UNIVERSITY OF THE WITWATERSRAND MEDICAL SCHOOL   (South Africa)

^b WESTAT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN P24; ANTIVIRUS AGENT; RNA DIRECTED DNA POLYMERASE; VIRUS RNA;

ARTICLE; ASSAY; CELL COUNT; CONTROLLED STUDY; CORRELATION ANALYSIS; COST EFFECTIVENESS ANALYSIS; DISEASE ACTIVITY; DISEASE MARKER; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; STATISTICAL SIGNIFICANCE; VIRUS LOAD;

ANTIRETROVIRAL THERAPY, HIGHLY ACTIVE; CD4 LYMPHOCYTE COUNT; HIV CORE PROTEIN P24; HIV INFECTIONS; HIV-1; HUMANS; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SOUTH AFRICA; VIRAL LOAD;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 13844280917     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.2.857-861.2005     Document Type: Article

References (23)

1. Awad, R. J., G. E. Corrigan, D. H. Ekstrand, R. Thorstensson, C. Kallander, and J. S. Gronowitz. 1997. Measurement of levels of human immunodeficiency virus type 1 reverse transcriptase (RT) and RT-activity-blocking antibody in humans by a new standardized colorimetric assay. J. Clin. Microbiol. 35:1080-1089.
2. Boni, J., M. Opravil, Z. Tomasik, M. Rothen, K. Bisset, P. Grob, R. Lüthy, and J. Schüpbach. 1997. Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV p24 antigen assay with heat-denatured plasma. AIDS 11:F47-F52.
3. Braun, J., J. C. Plantier, M. F. Hellot, E. Tuaillon, M. Gueudin, F. Damond, A. Malmsten, G. E. Corrigan, and F. Simon. 2003. A new quantitative HIV viral load assay based on plasma virion reverse transcriptase activity for the different types, groups and subtypes. AIDS 17:331-336.
4. Brown, P. 2000. Cheaper AIDS drugs due for Third World. Nature 405:263.
5. Burgisser, P., P. Vernazza, M. Flepp, J. Boni, Z. Tomasik, U. Hummel, G. Pantaleo, and J. Schupbach. 2000. Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV cohort study. J. Acquir. Immune Defic. Syndr. 23:138-144.
6. Department of Health. 2000. National HIV sero-prevalence survey of women attending public antenatal clinics in South Africa. Directorate, Health Systems Research and Epidemiology, Department of Health, Pretoria, South Africa.
7. Diggle, P. J., K.-Y. Liang, and S. L. Zeger. 1994. Analysis of longitudinal data. Clarendon Press, Oxford, United Kingdom.
8. Garcia, J. G., S. Yamamoto, M. Gomez-Cano, V. Soriano, T. A. Green, M. P. Busch, T. M. Folks, and W. Heneine. 1998. Measurement of human immunodeficiency virus type-1 plasma virus load based on reverse transcriptase (RT) activity: evidence of variability of virion-associated RT. J. Infect. Dis. 177:1221-1229.
9. Glencross, D. K., B. V. Mendelow, and W. S. Stevens. 2003. Laboratory monitoring of HIV/AIDS in a resource-poor setting. S. Afr. Med. J. 93:262-263.
10. + T cell counts and HIV type 1 viral loads in untreated, infected adolescents. AIDS Res. Hum. Retrovir. 16:959-963.
11. Human Sciences Research Council of South Africa. 2002. The Nelson Mandela/SHRC study of HIV/AIDS, South African national HIV prevalence, behavioral risks and mass media, household survey 2002. Human Sciences Research Council of South Africa, Cape Town, South Africa.
12. Johnson, D. 2000. Principles of treating HIV. S. Afr. Med. J. 90:227-230.
13. Joint United Nations Programme on HIV/AIDS. 2002. UNAIDS report on the global HIV/AIDS epidemic. Joint United Nations Programme on HIV/AIDS, Geneva, Switzerland.
14. Ledergerber, B., M. Flepp, J. Boni, Z. Tomasik, R. W. Cone, R. Lüthy, and J. Schüpbach. 2000. Human immunodeficiency virus type 1 p24 concentration measured by boosted ELISA of heat-denatured plasma correlates with decline in CD4 cells, progression to AIDS, and survival: comparison with viral RNA measurement. J. Infect. Dis. 181:1280-1288.
15. Malmsten, A., X. W. Shao, K. Aperia, G. E. Corrigan, E. Sandstrom, C. F. Kallander, T. Leitner, and J. S. Gronowitz. 2003. HIV-1 viral load determination based on reverse transcriptase activity from human plasma. J. Med. Virol. 71:347-359.
16. Mellors, J. W., C. W. Rinaldo, P. Gupta, R. M. White, J. A. Todd, and L. A. Kingsley. 1996. Prognosis of HIV infection predicted by the quantity of virus in plasma. Science 272:1167-1170.
17. Nadal, D., J. Boni, C. Kind, O. E. Varnier, F. Steiner, Z. Tomasik, and J. Schupbach. 1999. Prospective evaluation of amplification boosted ELISA for heat-denatured p24 antigen for the diagnosis and monitoring of pediatric human immunodeficiency virus type I infection. J. Infect. Dis. 180:1089-1095.
18. Schupbach, J., J. Boni, Z. Tomasik, J. Jendis, R. Seger, C. Kind, et al. 1994. Sensitive detection and early prognostic significance of p24 antigen in heat-denatured plasma of human immunodeficiency virus type 1 infected infants. J. Infect. Dis. 170:318-324.
19. Schüpbach, J., and J. Boni. 1993. Quantitative and sensitive detection of immune-complexed and free HIV antigen after boiling of serum. J. Virol. Methods 43:247-256. (Erratum, 45:245.)
20. Schüpbach, J., M. Flepp, D. Pontelli, Z. Tomasik, R. Lüthy, and J. Boni. 1996. Heat mediated immune complex dissociation and enzyme linked immunosorbent assay signal amplification render p24 antigen detection in plasma as sensitive as HIV-1 RNA detection by polymerase chain reaction. AIDS 10:1085-1090.
21. Schupbach, J., J. Boni, L. R. Bisset, Z. Tomasik, M. Fischer, H. F. Gunthard, B. Ledergerber, M. Opravil, and the Swiss HIV Cohort Study. 2003. HIV-1 p24 antigen is a significant inverse correlate of CD4 T-cell change in patients with suppressed viremia under long-term antiretroviral therapy. J. Acquir. Immune Defic. Syndr. 33:292-299.
22. Snedecor, G. W., and W. G. Cochran. 1989. Statistical methods, 8th ed. Iowa State University Press, Ames.
23. World Health Organization. 2002. Scaling up antiretroviral therapy in resource limited settings. WHO guidelines. WHO document, April 2002. http://www.who.int/entity/hiv/topics/arv/en/scaling_exe_summary.pdf. World Health Organization, Geneva, Switzerland.