Development of a green fluorescent tagged strain of Aspergillus carbonarius to monitor fungal colonization in grapes

International Journal of Food Microbiology

Volumn 148, Issue 2, 2011, Pages 135-140

  Crespo Sempere, A ^a,b   Lopez Perez M ^a   Martinez Culebras P V ^a,b   Gonzalez Candelas L ^a  

^a INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Aspergillus carbonarius; ATMT; Confocal microscopy; Grapes; Green fluorescent protein; Ochratoxin production; Wine

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYGROMYCIN; OCHRATOXIN;

ARTICLE; ASPERGILLUS CARBONARIUS; COCULTURE; CONFOCAL LASER MICROSCOPY; CONFOCAL MICROSCOPY; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS GROWTH; FUNGUS HYPHAE; FUNGUS ISOLATION; GENE EXPRESSION; GENETIC VARIABILITY; GRAPE; NONHUMAN; POLYMERASE CHAIN REACTION; RHIZOBIUM RADIOBACTER; WINE;

ASPERGILLUS; CINNAMATES; CONSUMER PRODUCT SAFETY; DNA, FUNGAL; FOOD CONTAMINATION; FRUIT; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HYGROMYCIN B; HYPHAE; MICROSCOPY, CONFOCAL; OCHRATOXINS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; RHIZOBIUM RADIOBACTER; SPORES, FUNGAL; TRANSFORMATION, GENETIC; VITIS;

AGROBACTERIUM TUMEFACIENS; ASPERGILLUS; ASPERGILLUS CARBONARIUS; FUNGI; VITACEAE;

EID: 79959492257     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.05.021     Document Type: Article

References (33)

1. Battilani P., Logrieco A., Giorni P., Cozzi G., Bertuzzi T., Pietri A. Ochratoxin A production by Aspergillus carbonarius on some grape varieties grown in Italy. Journal of the Science of Food and Agriculture 2004, 84:1736-1740.
2. Battilani P., Magan N., Logrieco A. European research on ochratoxin A in grapes and wine. International Journal of Food Microbiology 2006, 111(Suppl 1):S2-S4.
3. Bau M., Bragulat M.R., Abarca M.L., Minguez S., Cabanes F.J. Ochratoxigenic species from Spanish wine grapes. International Journal of Food Microbiology 2005, 98:125-130.
4. Bellí N., Pardo E., Marin S., Farre G., Ramos A.J., Sanchis V. Occurrence of ochratoxin A and toxigenic potential of fungal isolates from Spanish grapes. Journal of Science of Food and Agriculture 2004, 84:541-546.
5. Bellí N., Marín S., Coronas I., Sanchis V., Ramos A.J. Skin damage, high temperature and relative humidity as detrimental factors for Aspergillus carbonarius infection and ochratoxin A production in grapes. Food Control 2007, 18:1343-1349.
6. Bragulat M.R., Abarca M.L., Cabanes F.J. An easy screening method for fungi producing ochratoxin A in pure culture. International Journal of Food Microbiology 2001, 71:139-144.
7. Chalfie M., Tu Y., Euskirchen G., Ward W., Prasher D. Green fluorescent protein as a marker for gene expression. Science 1994, 263:802-805.
8. Chulze S.N., Magnoli C.E., Dalcero A.M. Occurrence of ochratoxin A in wine and ochratoxigenic mycoflora in grapes and dried vine fruits in South America. International Journal of Food Microbiology 2006, 111:S5-S9.
9. Cormack B.P., Valdivia R.H., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173:33-38.
10. Creppy E.E. Human ochratoxicoses. Journal of Toxicology: Toxin Reviews 1999, 18:273-293.
11. Crespo-Sempere A., González-Candelas L., Martínez-Culebras P.V. Genes differentially expressed by Aspergillus carbonarius strains under ochratoxin A producing conditions. International Journal of Food Microbiology 2010, 142:170-179.
12. Czymmek K.J., Bourett T.M., Howard R.J. Fluorescent protein probes in fungi. Methods in Microbiology 2004, 27-62. Academic Press. T. Savidge, P. Charalabos (Eds.).
13. De Groot M.J.A., Bundock P., Hooykaas P.J.J., Beijersbergen A.G.M. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nature Biotechnology 1998, 16:839-842.
14. Frandsen R.J.N., Andersson J.A., Kristensen M.B., Giese H. Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi. BMC Molecular Biology 2008, 9.
15. Kazi B.A., Emmett R.W., Nancarrow N., Partington D.L. Berry infection and the development of bunch rot in grapes caused by Aspergillus carbonarius. Plant Pathology 2008, 57:301-307.
16. Kuiper-Goodman T., Scott P.M. Risk assessment of the mycotoxin ochratoxin A. Biomedical and Environmental Science 1989, 2:179-248.
17. Leong S.-L.L., Hocking A.D., Pitt J.I., Kazi B.A., Emmett R.W., Scott E.S. Black Aspergillus species in Australian vineyards: from soil to ochratoxin A in wine. Advances in Food Mycology 2006, 153-171. Springer, US. A.D. Hocking, J.I. Pitt, R.A. Samson, U. Thrane (Eds.).
18. Lorang J.M., Tuori R.P., Martinez J.P., Sawyer T.L., Redman R.S., Rollins J.A., Wolpert T.J., Johnson K.B., Rodriguez R.J., Dickman M.B., Ciuffetti L.M. Green fluorescent protein is lighting up fungal biology. Applied Environmental Microbiology 2001, 67:1987-1994.
19. Martínez-Culebras P.V., Ramon D. An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine. International Journal of Food Microbiology 2007, 113:147-153.
20. Martínez-Culebras P.V., Crespo-Sempere A., Sánchez-Hervás M., Elizaquivel P., Aznar R., Ramón D. Molecular characterization of the black Aspergillus isolates responsible for ochratoxin A contamination in grapes and wine in relation to taxonomy of Aspergillus section Nigri. International Journal of Food Microbiology 2009, 132:33-41.
21. Morioka L.R.I., Furlaneto M.C., Bogas A.C., Pompermayer P., Duarte R.T.D., Vieira M.L.C., Watanabe M.A.E., Fungaro M.H.P. Efficient genetic transformation system for the ochratoxigenic fungus Aspergillus carbonarius. Current Microbiology 2006, 52:469-472.
22. Oueslati S., Lasram S., Ramos A.J., Marin S., Mliki A., Sanchis V., Ghorbel A. Alternating temperatures and photoperiod effects on fungal growth and Ochratoxin A production by Aspergillus carbonarius isolated from Tunisian grapes. International Journal of Food Microbiology 2010, 139:210-213.
23. Peng X.-L., Xu W.-T., Wang Y., Huang K.-L., Liang Z.-h., Zhao W.-w., Luo Y.-B. Mycotoxin Ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana. Plant Cell Reports 2010, 29:153-161.
24. Perrone G., Susca A., Cozzi G., Ehrlich K., Varga J., Frisvad J.C., Meijer M., Noonim P., Mahakarnchanakul W., Samson R.A. Biodiversity of Aspergillus species in some important agricultural products. Studies in Mycology 2007, 59:53-66.
25. Petzinger E., Ziegler K. Ochratoxin A from a toxicological perspective. Journal of Veterinary Pharmacology and Therapeutics 2000, 23:91-98.
26. Pfohl-Leszkowicz A., Manderville R.A. Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Molecular Nutrition & Food Research 2007, 51:61-99.
27. Pitt J.I., Hocking A.D. Fungi and food spoilage 1997, Blackie Academic and Professional, London.
28. Pliego C., Kanematsu S., Ruano-Rosa D., de Vicente A., López-Herrera C., Cazorla F.M., Ramos C. GFP sheds light on the infection process of avocado roots by Rosellinia necatrix. Fungal Genetics and Biology 2009, 46:137-145.
29. Rajasekaran K., Cary J., Cotty P., Cleveland T. Development of a GFP-expressing Aspergillus flavus strain to study fungal invasion, colonization, and resistance in cottonseed. Mycopathologia 2008, 165:89-97.
30. Reverberi M., Ricelli A., Zjalic S., Fabbri A., Fanelli C. Natural functions of mycotoxins and control of their biosynthesis in fungi. Applied Microbiology and Biotechnology 2010, 87:899-911.
31. Schmidt-Heydt M., Schunck T., Geisen R. Expression of a gfp gene in Penicillium nordicum under control of the promoter of the ochratoxin A polyketide synthase gene. International Journal of Food Microbiology 2009, 133:161-166.
32. Serra R., Mendonça C., Venâncio A. Fungi and ochratoxin A detected in healthy grapes for wine production. Letters in Applied Microbiology 2006, 42:42-47.
33. Zhang L., Yang J., Niu Q., Zhao X., Ye F., Liang L., Zhang K.-Q. Investigation on the infection mechanism of the fungus Clonostachys rosea against nematodes using the green fluorescent protein. Applied Microbiology and Biotechnology 2008, 78:983-990.