Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension

Methods in Molecular Biology

Volumn 729, Issue , 2011, Pages 153-166

  Gratz, Andreas ^a   Jose, Joachim ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Directed evolution; Error prone PCR; EstE; Esterase; Library; Random mutagenesis; SOE PCR

Indexed keywords

PEPTIDE LIBRARY;

AMINO ACID SEQUENCE; ARTICLE; METHODOLOGY; MOLECULAR EVOLUTION; MOLECULAR GENETICS; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; EVOLUTION, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, TERTIARY;

EID: 79958272586     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-065-2_10     Document Type: Chapter

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