Protein hydrolysis by immobilized and stabilized trypsin

Biotechnology Progress

Volumn 27, Issue 3, 2011, Pages 677-683

  Marques, Daniela ^a,b   Pessela, Benavides C ^c   Betancor, Lorena ^d   Monti, Rubens ^b   Carrascosa, Alfonso V ^a   Rocha Martin, Javier ^c   Guisán, Jose M ^a   Fernandez Lorente, Gloria ^c  

^a CSIC   (Spain)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^c INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

^d UNIVERSIDAD ORT URUGUAY   (Uruguay)

Author keywords

Cheese whey hydrolysis; Multichain immobilization of trypsin; Two step multipoint enzyme immobilization

Indexed keywords

ACTIVE SITE; AMINO GROUP; CHEESE WHEY; CHEESE WHEY HYDROLYSIS; COVALENT IMMOBILIZATION; EXPERIMENTAL CONDITIONS; HIGH CONCENTRATION; HIGH MOLECULAR WEIGHT; HIGH MOLECULAR WEIGHT PROTEIN; IMMOBILIZED BIOCATALYSTS; IMMOBILIZED TRYPSIN; INITIAL ACTIVITY; MULTICHAIN IMMOBILIZATION OF TRYPSIN; MULTIPOINT; MULTIPOINT IMMOBILIZATION; PROTEIN HYDROLYSIS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBLE ENZYMES; SURFACE REGION; SYNTHETIC SUBSTRATES; TERMINAL GROUPS; THERMALLY STABLE; THREE DIMENSIONAL (3D) STRUCTURES; TWO-STEP MULTIPOINT ENZYME IMMOBILIZATION;

ALDEHYDES; ALKALINITY; AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; CATALYST ACTIVITY; ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; HYDROLYSIS; MOLECULAR WEIGHT; PROTEINS; PROTEOLYSIS; SODIUM; SODIUM SULFATE; THREE DIMENSIONAL;

ENZYME IMMOBILIZATION;

IMMOBILIZED ENZYME; PROTEIN; TRYPSIN;

ARTICLE; BINDING SITE; BIOCATALYSIS; CHEMISTRY; HYDROLYSIS; KINETICS; METABOLISM; PH; PROTEIN CONFORMATION; PROTEIN STABILITY; SYNTHESIS;

BINDING SITES; BIOCATALYSIS; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; PROTEIN CONFORMATION; PROTEIN STABILITY; PROTEINS; TRYPSIN;

EID: 79958245094     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.600     Document Type: Article

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