Chemically mimicked hypoxia modulates gene expression and protein levels of the sodium calcium exchanger in HEK 293 cell line via HIF-1α

General Physiology and Biophysics

Volumn 30, Issue 2, 2011, Pages 196-206

  Hudecova, Sona ^a   Lencesova, Lubomira ^a,b   Csaderova, Lucia ^b   Sirova, Marta ^a   Cholujova, Dana ^c   Cagala, Martin ^a   Kopacek, Juraj ^d   Dobrota, Dusan ^e   Pastorekova, Silvia ^b,d   Krizanova, Olga ^a  

^a INSTITUTE OF MOLECULAR PHYSIOLOGY AND GENETICS   (Slovakia)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

^c CANCER RESEARCH INSTITUTE   (Slovakia)

^d INSTITUTE OF VIROLOGY   (Slovakia)

^e University Teaching Hospital in Martin   (Slovakia)

Author keywords

Apoptosis; DMOG; Sodium calcium exchanger

Indexed keywords

DIMETHYLOXALLYL GLYCINE; GLYCINE DERIVATIVE; HYPOXIA INDUCIBLE FACTOR 1ALPHA; LUCIFERASE; MESSENGER RNA; SODIUM CALCIUM EXCHANGE PROTEIN; SODIUM CALCIUM EXCHANGE PROTEIN 1; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL HYPOXIA; CELL PH; CELL STRAIN HEK293; CONTROLLED STUDY; EMBRYO; FIBROBLAST; GENE EXPRESSION REGULATION; GENE INDUCTION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INDUCTION; REPORTER GENE; UPREGULATION;

ANIMALS; ANNEXIN A5; ANOXIA; APOPTOSIS; FIBROBLASTS; FLUORESCENT DYES; GENE EXPRESSION REGULATION; HEK293 CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; MICE; MICROSCOPY, FLUORESCENCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SODIUM-CALCIUM EXCHANGER;

EID: 79958224743     PISSN: 02315882     EISSN: None     Source Type: Journal    
DOI: 10.4149/gpb_2011_02_196     Document Type: Article

References (30)

1. Boscia F., Gala R., Pannaccione A., Secondo A., Scorziello A., Di Renzo G., Annunziato L. (2009): NCX1 expression and functional activity increase in microglia invading the infarct core. Stroke 11, 3608-3617 doi:10.1161/STROKEAHA. 109.557439
2. Calvert J. W., Cahill J., Yamaquchi-Okada M., Zhang J. H. (2006): Oxygen treatment after experimental hypoxia-ischemia in neonatal rats afters the expression of HIF-1 alpha and its downstream target genes. J. Appl. Physiol. 10, 853-865 doi:10.1152/japplphysiol.00268.2006
3. Correia S. C., Moreira P. I. (2010): Hypoxia-inducible factor 1: a new hope to counteract neurodegeneration? J. Neurochem. 112, 1-12 doi:10.1111/j.1471-4159.2009.06443.x
4. Cummins E. P., Berra E., Comerford K. M., Ginouves A., Fitzgerald K. T., Seeballuck F., Godson C., Nielsen J. E., Moynagh P., Pouyssegur J., Taylor C. T. (2006): Prolyl hydroxylase-1 negatively regulates IkappaB kinase-beta, giving insight into hypoxia-induced NFkappaB activity. Proc. Natl. Acad. Sci. U.S.A 103, 18154-18159 doi:10.1073/pnas.0602235103
5. Diaz-Horta O., Kamagate A., Herchuelz A., Van Eylen F. (2002): Na+/Ca2+ exchanger overexpression induces endoplasmic reticulum-related apoptosis and caspase-12 activation in insulin-releasing BRIN-BD11 cells. Diabetes 51, 1815-1824 doi:10.2337/diabetes.51.6.1815 (Pubitemid 34564294)
6. Eigel B. N., Gursahani H., Hadley R. W. (2004): ROS are required for rapid reactivation of Na+/Ca2+ exchanger in hypoxic reoxygenated guinea pig ventricular myocytes. Am. J. Physiol. Heart. Circ. Physiol. 286, H955-963 doi:10.1152/ajpheart.00721.2003
7. Fan X., Heijnen C. J., van der Kooij M. A., Groenendaal F., van Bel F. (2009): The role and regulation of hypoxia-inducible factor-1 alpha expression in brain development and neonatal hypoxic-ischemic brain injury. Brain. Res. Rev. 62, 99-108 doi:10.1016/j.brainresrev.2009.09.006
8. Hudecova S., Tillinger A., Mravec B., Kvetnansky R., Krizanova O. (2004a): Effect of 6-hydroxydopamine on the gene expression of Na+/Ca2+ exchanger in the rat heart. Gen. Physiol. Biophys. 23, 307-313
9. Hudecova S., Vadaszova A., Soukup T., Krizanova O. (2004b): Effect of thyroid hormones on the gene expression of calcium transport systems in rat muscle. Life Sci. 75, 923-931 doi:10.1016/j.lfs.2004.01.026 (Pubitemid 38749251)
10. Hudecova S., Kubovcakova L., Kvetnansky R., Kopacek J., Pastorekova S., Novakova M., Knezl V., Tarabova B., Lacinova L., Sulova Z., Breier A., Jurkovicova D., Krizanova O. (2007): Modulation of expression of Na+/Ca2+ exchanger in heart of rat and mouse under stress. Acta Physiol. (Oxf) 190, 127-136 doi:10.1111/j.1748-1716.2007.01673.x
11. Jurkovicova D., Kopacek J., Stefanik P., Kubovcakova L., Zahradnikova A. Jr., Zahradnikova A., Pastorekova S., Krizanova O. (2007): Hypoxia modulates gene expression of IP3 receptors in rodent cerebellum. Pflugers Arch. 454, 415-425
12. Katschinski D. M., Le L., Heinrich D., Wagner K. F., Hofer T., Schindler S. G., Wenger R. H. (2002): Heat induction of the unphosphorylated form of hypoxia-inducible factor-1alpha is dependent on heat shock protein-90 activity. J. Biol. Chem. 277, 9262-9267 doi:10.1074/jbc.M110377200
13. Li Z., Matsuoka S., Hryshko L. V., Nicoll D. A., Bersohn M. M., Burke E. P., Lifton R. P., Philipson K. D. (1994): Cloning of the NCX2 isoform of the plasma membrane Na+-Ca2+ exchanger. J. Biol. Chem. 269, 17434-17439
14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
15. Miyamoto S., Howes A. L., Adams J. W., Dorn G. W. 2nd, Brown J. H. (2005): Ca2+ dysregulation induces mitochondrial depolarization and apoptosis: role of Na+/Ca2+ exchanger and AKT. J. Biol. Chem. 280, 38505-38512 doi:10.1074/jbc.M505223200
16. Molinaro P., Cuomo O., Pignataro G., Boscia F., Sirabella R., Pannaccione A., Secondo A., Scorziello A., Adornetto A., Gala R., Viggiano D., Sokolow S., Herchuelz A., Schurmans S., Di Renzo G., Annunziato L. (2008): Targeted disruption of Na+/Ca2+ exchanger 3 (NCX3) gene leads to a worsening of ischemic brain damage. J. Neurosci. 28, 1179-1184 doi:10.1523/JNEUROSCI.4671-07.2008
17. Nagano T., Osakada M., Ago Y., Koyama Y., Baba A., Maeda S., Takemura M., Matsuda T. (2005): SEA0400, a specific inhibitor of the Na+-Ca2+ exchanger, attenuates sodium nitroprusside-induced apoptosis in cultured rat microglia. Br. J. Pharmacol. 144, 669-679 doi:10.1038/sj.bjp.0706104
18. Nicoll D. A., Quednau B. D., Qui Z., Xia Y. R., Lusis A. J., Philipson K. D. (1996): Cloning of a third mammalian Na+-Ca2+ exchanger. NCX3. J. Biol. Chem. 271, 24914-24921 doi:10.1074/jbc.271.40.24914
19. Ryan H. E., Poloni M., McNulty W., Elson D., Gassmann M., Arbeit J. M., Johnson R. S. (2000): Hypoxia-inducible factor-1alpha is a positive factor in solid tumor growth. Cancer Res. 60, 4010-4015 (Pubitemid 30636574)
20. Ryan S., Taylor C. T., McNicholas W. T. (2005): Selective activation of inflammatory pathways by intermittent hypoxia in obstructive sleep apnea syndrome. Circ. 112, 2660-2667 doi:10.1161/CIRCULATIONAHA.105.556746 (Pubitemid 41532603)
21. Seagroves T. N., Ryan H. E., Lu H., Wouters B. G., Knapp M., Thibault P., Laderoute K., Johnson R. S. (2001): Transcription factor HIF-1 is a necessary mediator of the pasteur effect in mammalian cells. Mol. Cell. Biol. 21, 3436-3444 doi:10.1128/MCB.21.10.3436-3444.2001
22. Semenza G. L. (2000): HIF-1: mediator of physiological and pathophysiological responses to hypoxia. J. Appl. Physiol. 88, 1474-1480
23. Shono Y., Kamouchi M., Kitazono T., Kuroda J., Nakamura K., Hagiwara N., Ooboshi H., Ibayashi S., Iida M. (2010): Change in pHi causes the toxic Ca2+ entry via NCX1 in neuron- and glia-derived cells. Cell. Mol. Neurobiol. 30, 453-460 doi:10.1007/s10571-009-9470-7
24. Siddiq A., Aminova L. R., Ratan R. R. (2008): Prolyl 4-hydroxylase activity-responsive transcription factors: from hydroxylation to gene expression and neuroprotection. Front. Biosci. 13, 2875-2887 doi:10.2741/2892
25. Sirabella R., Secondo A., Pannaccione A., Scorziello A., Valsecchi V., Adornetto A., Bilo L., di Renzo G., Annunziato L. (2009): Anoxia-induced NF-kappaB-dependent upregulation of NCX1 contributes to Ca2+ refiling into endoplasmic reticulum in cortical neurons. Stroke 40, 922-929 doi:10.1161/STROKEAHA.108.531962
26. Taylor C. T., Dzus A. L., Colgan S. P. (1998): Autocrine regulation of epithelial permeability by hypoxia: role for polarized release of tumor necrosis factor alpha. Gastroenterol. 114, 657-668 doi:10.1016/S0016-5085(98)70579-7 (Pubitemid 28160400)
27. Valsecchi V., Pignataro G., Del Prete A., Sirabella R., Matrone C., Boscia F., Scorziello A., Sisalli M. J., Esposito E., Zambrano N., Di Renzo G., Annunziato L. (2011): NCX1 is a novel target gene for hypoxia-inducible factor-1 in ischemic brain preconditioning. Stroke 42, 754-763 doi:10.1161/STROKEAHA. 110.597583
28. Wakimoto K., Kobayashi K., Kuro O. M., Yao A., Iwamoto T, Yanaka N., Kita S., Nishida A., Azuma S., Toyoda Y., Omori K., Imahie H., Oka T., Kudoh S., Kohmoto O., Yazaki Y., Shigekawa M., Imai Y., Nabeshima Y., Komuro I. (2000): Targeted disruption of Na+/Ca2+ exchanger gene leads to cardiomyocyte apoptosis and defects in heartbeat. J. Biol. Chem. 275, 36991-36998 doi:10.1074/jbc. M004035200
29. Yamashita J., Itoh M., Kuro T., Kobayashi Y., Ogata M., Takaoka M., Matsumura Y. (2001): Pre-ischemic or post-ischemic treatment with a novel Na+/Ca2+ exchange inhibitor, KB-R7943, shows renal protective effects in rats with ischemic acute renal failure. J. Pharmacol. Exp. Ther. 296, 412-419
30. Yamashita J., Kita S., Iwamoto T., Ogata M., Takaoka M., Tazawa N., Nishikawa M., Wakimoto K., Shigekawa M., Komuro I., Matsumura Y. (2003): Attenuation of ischemia/reperfusion- induced renal injury in mice deficient in Na+/Ca2+ exchanger. J. Pharmacol. Exp. Ther. 304, 284-293 doi:10.1124/jpet.102. 039024