Selectable genetic markers for nematode transgenesis

Cellular and Molecular Life Sciences

Volumn 68, Issue 11, 2011, Pages 1917-1927

  Giordano Santini, Rosina ^a   Dupuy, Denis ^a  

^a INSTITUT EUROPÉEN DE CHIMIE ET BIOLOGIE   (France)

Author keywords

Antibiotics; Caenorhabditis elegans; Genetics; Model organisms; Nematodes; Transgenesis

Indexed keywords

PHLEOMYCIN; PUROMYCIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ANTIMICROBIAL ACTIVITY; BIOPROCESS; CAENORHABDITIS ELEGANS; DNA DETERMINATION; DNA INTEGRATION; GENE INSERTION; GENE SEQUENCE; GENETIC MARKER; GENOMICS; NONHUMAN; REVIEW; TRANSGENIC ANIMAL; TRANSGENICS;

ANIMALS; CAENORHABDITIS ELEGANS; DRUG RESISTANCE, MICROBIAL; GENE TRANSFER TECHNIQUES; GENETIC MARKERS; MODELS, GENETIC;

CAENORHABDITIS ELEGANS; NEMATODA;

EID: 79958194479     PISSN: 1420682X     EISSN: 14209071     Source Type: Journal    
DOI: 10.1007/s00018-011-0670-1     Document Type: Review

References (89)

1. S Brenner 1974 The genetics of Caenorhabditis elegans Genetics 77 1 71 94 4366476 1:STN:280:DyaE2c3ntFWlsw%3D%3D
2. Riddle DL, Blumenthal T, Meyer BJ, Preiss JR (1997) C. elegans II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
3. DT Stinchcomb JE Shaw SH Carr D Hirsh 1985 Extrachromosomal DNA transformation of Caenorhabditis elegans Mol Cell Biol 5 12 3484 3496 3837845 1:CAS:528:DyaL28XivFyjtQ%3D%3D (Pubitemid 16233056)
4. A Fire RH Waterston 1989 Proper expression of myosin genes in transgenic nematodes EMBO J 8 11 3419 3428 2583105 1:CAS:528:DyaL1MXmt1KmtL8%3D (Pubitemid 19273572)
5. S Goetinck RH Waterston 1994 The Caenorhabditis elegans muscle-affecting gene unc-87 encodes a novel thin filament-associated protein J Cell Biol 127 1 79 93 7929573 10.1083/jcb.127.1.79 1:CAS:528:DyaK2cXmsVOktbc%3D (Pubitemid 24307658)
6. R Aronoff R Baran J Hodgkin 2001 Molecular identification of smg-4. required for mRNA surveillance in C. elegans Gene 268 1-2 153 164 11368911 10.1016/S0378-1119(01)00414-0 1:CAS:528:DC%2BD3MXjsFWrs7o%3D (Pubitemid 32463799)
7. JC Way M Chalfie 1988 mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans Cell 54 1 5 16 2898300 10.1016/0092-8674(88)90174-2 1:CAS:528:DyaL1MXlt1Wisw%3D%3D
8. S Stone JE Shaw 1993 A Caenorhabditis elegans act-4::lacZ fusion: use as a transformation marker and analysis of tissue-specific expression Gene 131 2 167 173 8406009 10.1016/0378-1119(93)90290-J 1:CAS:528:DyaK3sXmsVahtb0%3D
9. AM Labrousse MD Zappaterra DA Rube AM van der Bliek 1999 C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane Mol Cell 4 5 815 826 10619028 10.1016/S1097-2765(00)80391-3 1:CAS:528:DyaK1MXnslOisrk%3D
10. M Chalfie Y Tu G Euskirchen WW Ward DC Prasher 1994 Green fluorescent protein as a marker for gene expression Science 263 5148 802 805 8303295 10.1126/science.8303295 1:CAS:528:DyaK2cXitFWkurc%3D (Pubitemid 24093206)
11. A Fire K Kondo R Waterston 1990 Vectors for low copy transformation of C. elegans Nucleic Acids Res 18 14 4269 4270 2377476 10.1093/nar/18.14.4269 1:CAS:528:DyaK3cXlsVKitrk%3D (Pubitemid 20240243)
12. PD Baum G Garriga 1997 Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin mutants Neuron 19 1 51 62 9247263 10.1016/S0896-6273(00)80347-5 1:CAS:528:DyaK2sXltFCjsro%3D (Pubitemid 27332993)
13. J Yochem T Gu M Han 1998 A new marker for mosaic analysis in Caenorhabditis elegans indicates a fusion between hyp6 and hyp7, two major components of the hypodermis Genetics 149 3 1323 1334 9649523 1:CAS:528:DyaK1cXkslOntbc%3D (Pubitemid 28334526)
14. IA Hope 1991 'Promoter trapping' in Caenorhabditis elegans Development 113 2 399 408 1782857 1:CAS:528:DyaK38XhtVShsbg%3D
15. Hobert O, Loria P (2005) Uses of GFP in C. elegans. In: Chalfie M, Kain S (eds) Green fluorescent protein: properties, applications and protocols. Wiley, New York
16. T Bacaj S Shaham 2007 Temporal control of cell-specific transgene expression in Caenorhabditis elegans Genetics 176 4 2651 2655 17603102 10.1534/genetics.107.074369 1:CAS:528:DC%2BD2sXhtFGmsr3N (Pubitemid 47360690)
17. Boulin T, Etchberger JF, Hobert O (2006) Reporter gene fusions. WormBook. doi: 10.1895/wormbook.1.106.1
18. BE Vogel EM Hedgecock 2001 Hemicentin, a conserved extracellular member of the immunoglobulin superfamily, organizes epithelial and other cell attachments into oriented line-shaped junctions Development 128 6 883 894 11222143 1:CAS:528:DC%2BD3MXislaqurk%3D (Pubitemid 32288199)
19. PM Loria A Duke JB Rand O Hobert 2003 Two neuronal, nuclear-localized RNA binding proteins involved in synaptic transmission Curr Biol 13 15 1317 1323 12906792 10.1016/S0960-9822(03)00532-3 1:CAS:528:DC%2BD3sXmt1Kit74%3D (Pubitemid 36953305)
20. AS Gonzalez-Serricchio PW Sternberg 2006 Visualization of C. elegans transgenic arrays by GFP BMC Genet 7 36 16759392 10.1186/1471-2156-7-36 (Pubitemid 46277095)
21. H Hutter 2000 New ways to look at axons in Caenorhabditis elegans Microsc Res Tech 48 1 47 54 10620784 10.1002/(SICI)1097-0029(20000101)48:1<47::AID- JEMT6>3.0.CO;2-1 1:STN:280:DC%2BD3c%2FovVKqsQ%3D%3D (Pubitemid 30036097)
22. JG White DG Albertson MA Anness 1978 Connectivity changes in a class of motoneurone during the development of a nematode Nature 271 5647 764 766 625347 10.1038/271764a0 1:STN:280:DyaE1c7hsVWjsw%3D%3D (Pubitemid 8278348)
23. The C. elegans Sequencing Consortium (1998) Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 282(5396):2012-2018
24. D Dupuy N Bertin CA Hidalgo K Venkatesan D Tu D Lee J Rosenberg N Svrzikapa A Blanc A Carnec AR Carvunis R Pulak J Shingles J Reece-Hoyes R Hunt-Newbury R Viveiros WA Mohler M Tasan FP Roth C Le Peuch IA Hope R Johnsen DG Moerman AL Barabasi D Baillie M Vidal 2007 Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans Nat Biotechnol 25 6 663 668 17486083 10.1038/nbt1305 1:CAS:528:DC%2BD2sXmtlersro%3D (Pubitemid 46897203)
25. R Hunt-Newbury R Viveiros R Johnsen A Mah D Anastas L Fang E Halfnight D Lee J Lin A Lorch S McKay HM Okada J Pan AK Schulz D Tu K Wong Z Zhao A Alexeyenko T Burglin E Sonnhammer R Schnabel SJ Jones MA Marra DL Baillie DG Moerman 2007 High-throughput in vivo analysis of gene expression in Caenorhabditis elegans PLoS Biol 5 9 e237 17850180 10.1371/journal.pbio.0050237 (Pubitemid 47416596)
26. JS Reece-Hoyes J Shingles D Dupuy CA Grove AJ Walhout M Vidal IA Hope 2007 Insight into transcription factor gene duplication from Caenorhabditis elegans promoterome-driven expression patterns BMC Genomics 8 27 17244357 10.1186/1471-2164-8-27
27. SE Celniker LA Dillon MB Gerstein KC Gunsalus S Henikoff GH Karpen M Kellis EC Lai JD Lieb DM MacAlpine G Micklem F Piano M Snyder L Stein KP White RH Waterston 2009 Unlocking the secrets of the genome Nature 459 7249 927 930 19536255 10.1038/459927a 1:CAS:528:DC%2BD1MXnsVagu7k%3D
28. CC Mello JM Kramer D Stinchcomb V Ambros 1991 Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences EMBO J 10 12 3959 3970 1935914 1:CAS:528:DyaK38XhsVOm (Pubitemid 21905425)
29. A Fire 1986 Integrative transformation of Caenorhabditis elegans EMBO J 5 10 2673 2680 16453714 1:CAS:528:DyaL2sXhtlSn
30. V Praitis E Casey D Collar J Austin 2001 Creation of low-copy integrated transgenic lines in Caenorhabditis elegans Genetics 157 3 1217 1226 11238406 1:CAS:528:DC%2BD3MXisFyktLo%3D (Pubitemid 32225014)
31. T Wilm P Demel HU Koop H Schnabel R Schnabel 1999 Ballistic transformation of Caenorhabditis elegans Gene 229 1-2 31 35 10095101 10.1016/S0378-1119(99)00043-8 1:CAS:528:DyaK1MXit1Knu7w%3D (Pubitemid 29148304)
32. M Maduro D Pilgrim 1995 Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system Genetics 141 3 977 988 8582641 1:CAS:528:DyaK28Xht1Cltrk%3D
33. DV Clark DS Suleman KA Beckenbach EJ Gilchrist DL Baillie 1995 Molecular cloning and characterization of the dpy-20 gene of Caenorhabditis elegans Mol Gen Genet 247 3 367 378 7770042 10.1007/BF00293205 1:CAS:528:DyaK2MXmsVShtb8%3D
34. C Thacker JA Sheps AM Rose 2006 Caenorhabditis elegans dpy-5 is a cuticle procollagen processed by a proprotein convertase Cell Mol Life Sci 63 10 1193 1204 16649143 10.1007/s00018-006-6012-z 1:CAS:528:DC%2BD28XlvVeqt7Y%3D (Pubitemid 43939005)
35. M Granato H Schnabel R Schnabel 1994 pha-1, a selectable marker for gene transfer in C. elegans Nucleic Acids Res 22 9 1762 1763 8202383 10.1093/nar/22.9.1762 1:CAS:528:DyaK2cXlt1Khu7k%3D (Pubitemid 24155838)
36. SG Clark X Lu HR Horvitz 1994 The Caenorhabditis elegans locus lin-15, a negative regulator of a tyrosine kinase signaling pathway, encodes two different proteins Genetics 137 4 987 997 7982579 1:CAS:528:DyaK2MXmslWktg%3D%3D (Pubitemid 24235613)
37. A Fire D Albertson SW Harrison DG Moerman 1991 Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle Development 113 2 503 514 1782862 1:CAS:528:DyaK38XhtVSms7c%3D
38. JD Thatcher C Haun PG Okkema 1999 The DAF-3 Smad binds DNA and represses gene expression in the Caenorhabditis elegans pharynx Development 126 1 97 107 9834189 1:CAS:528:DyaK1MXpsVOmsg%3D%3D (Pubitemid 29045903)
39. J Polanowska JS Martin R Fisher T Scopa I Rae SJ Boulton 2004 Tandem immunoaffinity purification of protein complexes from Caenorhabditis elegans Biotechniques 36 5 778 780, 782 15152596 1:CAS:528:DC%2BD2cXktV2nu78%3D
40. C Mello A Fire 1995 DNA transformation Methods Cell Biol 48 451 482 8531738 10.1016/S0091-679X(08)61399-0 1:CAS:528:DyaK2MXps1Cmu74%3D
41. C Frokjaer-Jensen MW Davis CE Hopkins BJ Newman JM Thummel SP Olesen M Grunnet EM Jorgensen 2008 Single-copy insertion of transgenes in Caenorhabditis elegans Nat Genet 40 11 1375 1383 18953339 10.1038/ng.248 1:CAS:528: DC%2BD1cXhtlSkurbK
42. D Bazopoulou N Tavernarakis 2009 The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans Genetica 137 1 39 46 19343510 10.1007/s10709-009-9361-3 1:CAS:528:DC%2BD1MXptVGjsLk%3D
43. SN Cohen AC Chang HW Boyer RB Helling 1973 Construction of biologically functional bacterial plasmids in vitro Proc Natl Acad Sci USA 70 11 3240 3244 4594039 10.1073/pnas.70.11.3240 1:CAS:528:DyaE2cXksFGgug%3D%3D
44. J Davies A Jimenez 1980 A new selective agent for eukaryotic cloning vectors Am J Trop Med Hyg 29 5 Suppl 1089 1092 7001938 1:STN:280: DyaL3M%2FmtVSktg%3D%3D (Pubitemid 11240793)
45. A Jimenez J Davies 1980 Expression of a transposable antibiotic resistance element in Saccharomyces Nature 287 5785 869 871 6253817 10.1038/287869a0 1:CAS:528:DyaL3MXpvFKluw%3D%3D (Pubitemid 11214492)
46. A Gatignol M Baron G Tiraby 1987 Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae Mol Gen Genet 207 2-3 342 348 2441230 10.1007/BF00331599 1:CAS:528:DyaL2sXkslWqsLs%3D (Pubitemid 17086378)
47. M Kimura T Kamakura QZ Tao I Kaneko I Yamaguchi 1994 Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae Mol Gen Genet 242 2 121 129 8159161 10.1007/BF00391004 1:CAS:528:DyaK2cXlslWlsLw%3D (Pubitemid 24043211)
48. L Gritz J Davies 1983 Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae Gene 25 2-3 179 188 6319235 10.1016/0378-1119(83)90223-8 1:CAS:528:DyaL2cXpslChtA%3D%3D (Pubitemid 14204644)
49. F Colbere-Garapin F Horodniceanu P Kourilsky AC Garapin 1981 A new dominant hybrid selective marker for higher eukaryotic cells J Mol Biol 150 1 1 14 6271971 10.1016/0022-2836(81)90321-1 1:CAS:528:DyaL3MXmtFOhu78%3D
50. K Blochlinger H Diggelmann 1984 Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells Mol Cell Biol 4 12 2929 2931 6098829 1:CAS:528:DyaL2MXktVyrtA%3D%3D (Pubitemid 15106853)
51. S de la Luna I Soria D Pulido J Ortin A Jimenez 1988 Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker Gene 62 1 121 126 10.1016/0378-1119(88)90585-9 (Pubitemid 18062011)
52. TA Pfeifer DD Hegedus TA Grigliatti DA Theilmann 1997 Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines Gene 188 2 183 190 9133590 10.1016/S0378-1119(96)00756-1 1:CAS:528:DyaK2sXhsFCmsbs%3D (Pubitemid 27170992)
53. K Okano N Miyajima N Takada M Kobayashi H Maekawa 1992 Basic conditions for the drug selection and transient gene expression in the cultured cell line of Bombyx mori In Vitro Cell Dev Biol 28A 11-12 779 781 1483968 10.1007/BF02631067 1:CAS:528:DyaK3sXhvValt70%3D (Pubitemid 23054052)
54. MG Lopez V Alfonso E Carrillo O Taboga 2010 Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ- baculovirus occlusion and larval per os infectivity J Biotechnol 145 2 199 205 19896510 10.1016/j.jbiotec.2009.10.015 1:CAS:528:DC%2BC3cXpt12q
55. L Herrera-Estrella MD Block E Messens JP Hernalsteens MV Montagu J Schell 1983 Chimeric genes as dominant selectable markers in plant cells EMBO J 2 6 987 995 16453464 1:CAS:528:DyaL3sXltVShtL4%3D
56. P Perez G Tiraby J Kallerhoff J Perret 1989 Phleomycin resistance as a dominant selectable marker for plant cell transformation Plant Mol Biol 13 4 365 373 2485087 10.1007/BF00015548 1:CAS:528:DyaK3cXkvF2ksw%3D%3D
57. C Waldron EB Murphy JL Roberts GD Gustafson SL Armour SK Malcolm 1985 Resistance to hygromycin B: a new marker for plant transformation studies Plant Mol Biol 5 103 108 10.1007/BF00020092 1:CAS:528:DyaL28Xhs1Cq
58. PJ Punt RP Oliver MA Dingemanse PH Pouwels CA van den Hondel 1987 Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli Gene 56 1 117 124 2824287 10.1016/0378-1119(87)90164-8 1:CAS:528:DyaL2sXmtlSmsL0%3D
59. S Gastmann A Dunkler A Walther K Klein J Wendland 2007 A molecular toolbox for manipulating Eremothecium coryli Microbiol Res 162 4 299 307 17716882 10.1016/j.micres.2007.05.008 1:CAS:528:DC%2BD2sXhsVeksLfJ (Pubitemid 47496216)
60. D Cullen SA Leong LJ Wilson DJ Henner 1987 Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph Gene 57 1 21 26 3322945 10.1016/0378-1119(87)90172-7 1:CAS:528:DyaL1cXhsFeq (Pubitemid 17160003)
61. S Jain H Durand G Tiraby 1992 Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker Mol Gen Genet 234 3 489 493 1406595 10.1007/BF00538710 1:CAS:528:DyaK38Xmt1Ogt7g%3D
62. M Dubin W Nellen 2010 A versatile set of tagged expression vectors to monitor protein localisation and function in Dictyostelium Gene 465 1-2 1 8 20600701 10.1016/j.gene.2010.06.010 1:CAS:528:DC%2BC3cXhtVajsLbE
63. CF Brunk P Navas 1988 Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival Exp Cell Res 174 2 525 532 2892691 10.1016/0014-4827(88)90322-9 1:STN:280:DyaL1c7htF2muw%3D%3D (Pubitemid 18067996)
64. DJ Freedman SM Beverley 1993 Two more independent selectable markers for stable transfection of Leishmania Mol Biochem Parasitol 62 1 37 44 8114824 10.1016/0166-6851(93)90175-W 1:CAS:528:DyaK2cXntVChtQ%3D%3D
65. S Goyard SM Beverley 2000 Blasticidin resistance: a new independent marker for stable transfection of Leishmania Mol Biochem Parasitol 108 2 249 252 10838227 10.1016/S0166-6851(00)00210-3 1:CAS:528:DC%2BD3cXjs1Chtbs%3D (Pubitemid 30317454)
66. CE Suarez TF McElwain 2009 Stable expression of a GFP-BSD fusion protein in Babesia bovis merozoites Int J Parasitol 39 3 289 297 18831975 10.1016/j.ijpara.2008.08.006 1:CAS:528:DC%2BD1MXhtFert7c%3D
67. H Steller V Pirrotta 1985 A transposable P vector that confers selectable G418 resistance to Drosophila larvae EMBO J 4 1 167 171 16453599 1:CAS:528:DyaL2MXht1Kgurk%3D
68. LH Miller RK Sakai P Romans RW Gwadz P Kantoff HG Coon 1987 Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae Science 237 4816 779 781 3039658 10.1126/science.3039658 1:CAS:528: DyaL2sXlsVymtr4%3D (Pubitemid 17134523)
69. AC Morris P Eggleston JM Crampton 1989 Genetic transformation of the mosquito Aedes aegypti by micro-injection of DNA Med Vet Entomol 3 1 1 7 2519641 10.1111/j.1365-2915.1989.tb00467.x 1:STN:280:DyaK387gtlantA%3D%3D
70. V McGrane JO Carlson BR Miller BJ Beaty 1988 Microinjection of DNA into Aedes triseriatus ova and detection of integration Am J Trop Med Hyg 39 5 502 510 3195697 1:CAS:528:DyaL1MXntVClsg%3D%3D
71. AM Handler DA O'Brochta 1991 Prospects for gene transformation in insects Annu Rev Entomol 36 159 183 1848743 10.1146/annurev.en.36.010191.001111 1:CAS:528:DyaK3MXhtFGrt74%3D (Pubitemid 21903675)
72. R Giordano-Santini S Milstein N Svrzikapa D Tu R Johnsen D Baillie M Vidal D Dupuy 2010 An antibiotic selection marker for nematode transgenesis Nat Methods 7 9 721 723 20729841 10.1038/nmeth.1494 1:CAS:528:DC%2BC3cXhtVGgtbrM
73. JI Semple R Garcia-Verdugo B Lehner 2010 Rapid selection of transgenic C. elegans using antibiotic resistance Nat Methods 7 9 725 727 20729840 10.1038/nmeth.1495 1:CAS:528:DC%2BC3cXhtVGgtbzK
74. S Bar-Nun Y Shneyour JS Beckmann 1983 G-418, an elongation inhibitor of 80s ribosomes Biochim Biophys Acta 741 1 123 127 6193810 1:CAS:528: DyaL3sXmtVyhsLs%3D (Pubitemid 13010172)
75. N Tanaka H Yamaguchi H Umezawa 1963 Mechanism of action of phleomycin, a tumor-inhibitory antibiotic Biochem Biophys Res Commun 10 171 174 13984808 10.1016/0006-291X(63)90045-7 1:CAS:528:DyaF3sXktFWgt7w%3D
76. WG Kelly S Xu MK Montgomery A Fire 1997 Distinct requirements for somatic and germline expression of a generally expressed Caenorhabditis elegans gene Genetics 146 1 227 238 9136012 1:CAS:528:DyaK2sXmsVarsb8%3D (Pubitemid 27194401)
77. M Hammarlund MT Palfreyman S Watanabe S Olsen EM Jorgensen 2007 Open syntaxin docks synaptic vesicles PLoS Biol 5 8 e198 17645391 10.1371/journal.pbio.0050198 (Pubitemid 47328274)
78. C Frokjaer-Jensen MW Davis G Hollopeter J Taylor TW Harris P Nix R Lofgren M Prestgard-Duke M Bastiani DG Moerman EM Jorgensen 2010 Targeted gene deletions in C. elegans using transposon excision Nat Methods 7 6 451 453 20418868 10.1038/nmeth.1454 1:CAS:528:DC%2BC3cXltFKlsLc%3D
79. X Li HC Massey Jr TJ Nolan GA Schad K Kraus M Sundaram JB Lok 2006 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements Int J Parasitol 36 6 671 679 16500658 10.1016/j.ijpara.2005.12.007 1:CAS:528:DC%2BD28XkvVynsb8%3D (Pubitemid 43765724)
80. B Schlager X Wang G Braach RJ Sommer 2009 Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus Genesis 47 5 300 304 19298013 10.1002/dvg.20499 1:CAS:528:DC%2BD1MXnslKlsb0%3D
81. K Shiomi MJA Omura 2004 Antiparasitic agents produced by microorganisms Proc Jpn Acad 80 245 258 10.2183/pjab.80.245 1:CAS:528:DC%2BD2cXnsFSju7o%3D (Pubitemid 39351116)
82. WN Grant SJ Skinner J Newton-Howes K Grant G Shuttleworth DD Heath CB Shoemaker 2006 Heritable transgenesis of Parastrongyloides trichosuri: a nematode parasite of mammals Int J Parasitol 36 4 475 483 16500659 10.1016/j.ijpara.2005.12.002 1:CAS:528:DC%2BD28XkvVynsL0%3D (Pubitemid 43744425)
83. S Hashmi G Hashmi R Gaugler 1995 Genetic transformation of an entomopathogenic nematode by microinjection J Invertebr Pathol 66 3 293 296 8568284 10.1006/jipa.1995.1103 1:STN:280:DyaK28%2Fnt1Cmug%3D%3D
84. JC Corbo M Levine RW Zeller 1997 Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian Ciona intestinalis Development 124 3 589 602 9043074 1:CAS:528:DyaK2sXhtlelu7w%3D (Pubitemid 27092425)
85. DW Wilson BS Crabb JG Beeson 2010 Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays Malar J 9 152 20525251 10.1186/1475-2875-9-152
86. T Matsuoka S Awazu E Shoguchi N Satoh Y Sasakura 2005 Germline transgenesis of the ascidian Ciona intestinalis by electroporation Genesis 41 2 67 72 15682387 10.1002/gene.20096 1:CAS:528:DC%2BD2MXjtFSrur8%3D (Pubitemid 40365660)
87. X-Y Guo L Dong S-P Wang T-Q Guo J-Y Wang C-D Lu 2004 Introduction of foreign genes into silkworm eggs by electroporation and its application in transgenic vector test Acta Biochim Biophys Sin 36 5 323 330 15156273 10.1093/abbs/36.5.323 (Pubitemid 39107280)
88. B Maher 2009 Evolution: biology's next top model? Nature 458 7239 695 698 19360058 10.1038/458695a
89. HM Chamberlin 2010 C. elegans select Nat Methods 7 9 693 695 20805797 10.1038/nmeth0910-693 1:CAS:528:DC%2BC3cXhtV2hsbjO