Tandem immunoaffinity purification of protein complexes from Caenorhabditis elegans

BioTechniques

Volumn 36, Issue 5, 2004, Pages 778-782

  Polanowska, Jolanta ^a   Martin, Julie S ^a   Fisher, Rhoda ^a   Scopa, Tina ^a   Rae, Ian ^a   Boulton, Simon J ^a,b  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b London Research Institute   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; IMMUNOAFFINITY CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PROTEIN PURIFICATION;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; INVERTEBRATA;

EID: 2442438994     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04365bm05     Document Type: Article

References (9)

1. Rigaut, G., A. Shevchenko, B. Rutz, M. Wilm, M. Mann, and B. Seraphin. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17:1030-1032.
2. Deshaies, R.J., J.H. Seol, W.H. McDonald, G. Cope, S. Lyapina, A. Shevchenko, R. Verma, and J.R. Yates, 3rd. 2002. Charting the protein complexome in yeast by mass spectrometry. Mol. Cell Proteomics 1:3-10.
3. Gavin, A.C., M. Bosche, R. Krause, P. Grandi, M. Marzioch, A. Bauer, J. Schultz, J.M. Rick, et al. 2002. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415:141-147.
4. Ho, Y., A. Gruhler, A. Heilbut, G.D. Bader, L. Moore, S.L. Adams, A. Millar, P. Taylor, et al. 2002. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature 415:180-183.
5. Shevchenko, A., D. Schaft, A. Roguev, W.W. Pijnappei, and A.F. Stewart. 2002. Deciphering protein complexes and protein interaction networks by tandem affinity purification and mass spectrometry: analytical perspective. Mol. Cell Proteomics 1:204-212.
6. Walhout, A.J., G.F. Temple, M.A. Brasch, J.L. Hartley, M.A. Lorson, S. van den Heuvel, and M. Vidal. 2000. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328:575-592.
7. Praltis, V., E. Casey, D. Collar, and J. Austin. 2001. Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157:1217-1226.
8. Brenner, S. 1974. The genetics of Caenorhabditis elegans. Genetics 77:71-94.
9. Shevchenko, A., M. Wilm, O. Vorm, and M. Mann. 1996. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68:850-858.