Purification, characterization and functional expression of a new peptide with an analgesic effect from Chinese scorpion Buthus martensii Karsch (BmK AGP-SYPU1)

Biomedical Chromatography

Volumn 25, Issue 7, 2011, Pages 801-807

  Wang, Yu ^a   Wang, Li ^a   Cui, Yong ^a   Song, Yong Bo ^a   Liu, Yan Feng ^a   Zhang, Rong ^a   Wu, Chun Fu ^a   Zhang, Jing Hai ^a  

^a SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Analgesic activity; BmK AGP SYPU1; Buthus martensii Karsch; Gene cloning; Purification; Recombinant expression

Indexed keywords

CLONING; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAMMALS; PEPTIDES;

ANALGESIC ACTIVITY; ANALGESIC EFFECT; BUTHUS MARTENSI KARSCH AGP-SYPU1; BUTHUS MARTENSII; BUTHUS MARTENSII KARSCH; CHROMATOGRAPHIC PROCESS; FUNCTIONAL EXPRESSION; GENE CLONING; PRIMARY STRUCTURES; RECOMBINANT EXPRESSION;

PURIFICATION;

AMINO ACID; ANALGESIC AGENT; BMK AGP SYPU1 PROTEIN; COMPLEMENTARY DNA; PROTEIN; SCORPION VENOM; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANALGESIC ACTIVITY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BINDING AFFINITY; BUTHUS MARTENSII KARSCH; CAPILLARY ELECTROPHORESIS; CHELATION; CONTROLLED STUDY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE SEQUENCE; GENOMICS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SCORPION;

ESCHERICHIA COLI; MESOBUTHUS MARTENSII; MUS; SCORPIONES;

EID: 79958187562     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1519     Document Type: Article

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