Defining the strain-dependent impact of the staphylococcal accessoryregulator (sarA) on the alpha-toxin phenotype of Staphylococcus aureus

Journal of Bacteriology

Volumn 193, Issue 12, 2011, Pages 2948-2958

  Zielinska, Agnieszka K ^a   Beenken, Karen E ^a   Joo, Hwang Soo ^b   Mrak, Lara N ^a   Griffin, Linda M ^a   Luong, Thanh T ^a   Lee, Chia Y ^a   Otto, Michael ^b   Shaw, Lindsey N ^c   Smeltzer, Mark S ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCESSORY GENE REGULATOR PROTEIN; ALPHA TOXIN; AUREOLYSIN; BACTERIAL PROTEIN; BACTERIAL RNA; BACTERIAL TOXIN; PHENOL SOLUBLE MODULIN; PROTEIN HLA; PROTEINASE; PROTEINASE INHIBITOR; REGULATOR PROTEIN; STAPHYLOCOCCAL ACCESSORY REGULATOR PROTEIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; GENETIC TRANSCRIPTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEGRADATION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; BACTERIAL TOXINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VARIATION; HEMOLYSIN PROTEINS; MUTATION; PHENOLS; PHENOTYPE; RNA, BACTERIAL; RNA, MESSENGER; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 79958041272     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.01517-10     Document Type: Article

References (46)

1. Beenken, K. E., J. S. Blevins, and M. S. Smeltzer. 2003. Mutation of sarA in Staphylococcus aureus limits biofilm formation. Infect. Immun. 71:4206- 4211.
2. Beenken, K. E., et al. 2010. Epistatic relationships between sarA and agr in Staphylococcus aureus biofilm formation. PLoS One 5:e10790.
3. Blevins, J. S., K. E. Beenken, M. O. Elasri, B. K. Hurlburt, and M. S. Smeltzer. 2002. Strain-dependent differences in the regulatory roles of sarA and agr in Staphylococcus aureus. Infect. Immun. 70:470-480.
4. Blevins, J. S., A. F. Gillaspy, T. M. Rechtin, B. K. Hurlburt, and M. S. Smeltzer. 1999. The staphylococcal accessory regulator (sar) controls expression of the Staphylococcus aureus collagen adhesin gene (cna) in an agrindependent manner. Mol. Microbiol. 33:317-326.
5. Boles, B. R., and A. R. Horswill. 2008. agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog. 4:e1000052.
6. Cassat, J. E., et al. 2005. Comparative genomics of Staphylococcus aureus musculoskeletal isolates. J. Bacteriol. 187:576-592.
7. Cassat, J. E., et al. 2006. Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390. Microbiology 152: 3075-3090.
8. Cheung, A. L., A. S. Bayer, G. Zhang, H. Gresham,X and Y.-Q. Xiong. 2004. Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus. FEMS Immunol. Med. Microbiol., 40:1-9.
9. Cheung, A. L., J. M. Koomey, C. A. Butler, S. J. Projan, and V. A. Fischetti. 1992. Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr. Proc. Natl. Acad Sci. U. S. A. 89:6462-6466.
10. Cheung, A. L., and S. J. Projan. 1994. Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr. J. Bacteriol. 176:4168-4172.
11. Chien, Y., A. C. Manna, and A. L. Cheung. 1998. SarA level is a determinant of agr activation in Staphylococcus aureus. Mol. Microbiol. 30:991-1001.
12. Chien, Y., A. C. Manna, S. J. Projan, and A. L. Cheung. 1999. SarA, a global regulator of virulence determinants in Staphylococcus aureus, binds to a conserved motif essential for sar-dependent gene regulation. J. Biol. Chem. 274:37169-37176.
13. DeLeo, F. R., M. Otto, B. N. Kreiswirth, and H. F. Chambers. 2010. Community-associated meticillin-resistant Staphylococcus aureus. Lancet 375: 1557-1568.
14. Diep, B. A., H. A. Carleton, R. F. Chang, G. F. Sensabaugh, and F. Perdreau-Remington. 2006. Roles of 34 virulence genes in the evolution of hospitaland community-associated strains of methicillin-resistant Staphylococcus aureus. J. Infect. Dis. 193:1495-1503.
15. Dunman, P. M., et al. 2001. Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci. J. Bacteriol. 183:7341-7353.
16. Gillaspy, A. F., et al. 1995. Role of the accessory gene regulator (agr) in the pathogenesis of staphylococcal osteomyelitis. Infect. Immun. 63:3373-3380.
17. Herbert, S., et al. 2010. Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates. Infect. Immun. 78:2877-2889.
18. B modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J. Bacteriol. 184:5457-5467.
19. Kernodle, D. S., R. K. Voladri, B. E. Menzies, C. C. Hager, and K. M. Edwards. 1997. Expression of an antisense hla fragment in Staphylococcus aureus reduces alpha-toxin production in vitro and attenuates lethal activity in a murine model. Infect. Immun. 65:179-184.
20. Kielian, T., A. Cheung, and W. F. Hickey. 2001. Diminished virulence of an alpha-toxin mutant of Staphylococcus aureus in experimental brain abscesses. Infect. Immun. 69:6902-6911.
21. Kobayashi, S. D., and F. R. DeLeo. 2009. An update on community-associated MRSA virulence. Curr. Opin. Pharmacol. 9:545-551.
22. Li, S., S. Arvidson, and R. Möllby. 1997. Variation in the agr-dependent expression of alpha-toxin and protein A among clinical isolates of Staphylococcus aureus from patients with septicaemia. FEMS Microbiol. Lett. 152: 155-161.
23. Lindsay, J. A., and S. J. Foster. 1999. Interactive regulatory pathways control virulence determinant production and stability in response to environmental conditions in Staphylococcus aureus. Mol. Gen. Genet. 262:323-331.
24. Luong, T. T., et al. 2011. Staphylococcus aureus ClpC divergently regulates capsule via sae and codY in strain Newman but activates capsule via codY in strain UAMS-1 and in strain Newman with repaired saeS. J. Bacteriol. 193:686-694.
25. McCallum, N., M. Bischoff, H. Maki, A. Wada, and B. Berger-Bachi. 2004. TcaR, a putative MarR-like regulator of sarS expression. J. Bacteriol. 186: 2966-2972.
26. McCormick, C. C., A. R. Caballero, C. L. Balzli, A. Tang, and R. J. O'Callaghan. 2009. Chemical inhibition of alpha-toxin, a key corneal virulence factor of Staphylococcus aureus. Invest. Ophthalmol. Vis. Sci. 50:2848-2854.
27. McElroy, M. C., et al. 1999. Alpha-toxin damages the air-blood barrier of the lung in a rat model of Staphylococcus aureus-induced pneumonia. Infect. Immun. 67:5541-5544.
28. Miller, K. D., D. L. Hetrick, and D. J. Bielefeldt. 1977. Production and properties of Staphylococcus aureus (strain Newman D2C) with uniform clumping factor activity. Thromb. Res. 10:203-211.
29. Montgomery, C. P., et al. 2008. Comparison of virulence in communityassociated methicillin-resistant Staphylococcus aureus pulsotypes USA300 and USA400 in a rat model of pneumonia. J. Infect. Dis. 198:561-570.
30. Morfeldt, E., D. Taylor, A. von Gabain, and S. Arvidson. 1995. Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA, RNAIII. EMBO J. 14:4569-4577.
31. Nilsson, I. M., O. Hartford, T. Foster, and A. Tarkowski. 1999. Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis. Infect. Immun. 67:1045-1049.
32. Novick, R. P., and D. Jiang. 2003. The staphylococcal saeRS system coordinates environmental signals with agr quorum sensing. Microbiology 149: 2709-2717.
33. Novick, R. P., et al. 1993. Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J. 12:3967-3975.
34. Oscarsson, J., A. Kanth, K. Tegmark-Wisell, and S. Arvidson. 2006. SarA is a repressor of hla (alpha-hemolysin) transcription in Staphylococcus aureus: its apparent role as an activator of hla in the prototype strain NCTC 8325 depends on reduced expression of sarS. J. Bacteriol. 188:8526-8533.
35. Phillips, J. R., T. J. Tripp, W. E. Regelmann, P. M. Schlievert, and O. D. Wangensteen. 2006. Staphylococcal alpha-toxin causes increased tracheal epithelial permeability. Pediatr. Pulmonol. 41:1146-1152.
36. Reed, S. B., et al. 2001. Molecular characterization of a novel Staphylococcus aureus serine protease operon. Infect. Immun. 69:1521-1527.
37. Rogasch, K., et al. 2006. Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains. J. Bacteriol. 188:7742-7758.
38. Schafer, D., et al. 2009. A point mutation in the sensor hisitidine kinase SaeS of Staphylococcus aureus strain Newman alters the response to biocide exposure. J. Bacteriol. 191:7306-7314.
39. Shaw, L., E. Golonka, J. Potempa, and S. J. Foster. 2004. The role and regulation of the extracellular proteases of Staphylococcus aureus. Microbiology 150:217-228.
40. Sifri, C. D., J. Park, G. A. Helm, M. E. Stemper, and S. K. Shukla. 2007. Fatal brain abscess due to community-associated methicillin-resistant Staphylococcus aureus strain USA300. Clin. Infect. Dis. 45:e113-e117.
41. Tenover, F. C., and R. V. Goering. 2009. Methicillin-resistant Staphylococcus aureus strain USA300: origin and epidemiology. J. Antimicrob. Chemother. 64:441-446.
42. Tenover, F. C., et al. 1994. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J. Clin. Microbiol. 32:407-415.
43. Tsang, L. H., J. E. Cassat, L. N. Shaw, K. E. Beenken, and M. S. Smeltzer. 2008. Factors contributing to the biofilm-deficient phenotype of Staphylococcus aureus sarA mutants. PLoS One 3:e3361.
44. Wang, R., et al. 2007. Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat. Med. 13:1510-1514.
45. Weiss, E. C., H. J. Spencer, S. J. Daily, B. D. Weiss, and M. S. Smeltzer. 2009. Impact of sarA on antibiotic susceptibility of Staphylococcus aureus in a catheter-associated in vitro model of biofilm formation. Antimicrob. Agents Chemother. 53:2475-2482.
46. Weiss, E. C., et al. 2009. Impact of sarA on daptomycin susceptibility of Staphylococcus aureus biofilms in vivo. Antimicrob. Agents Chemother. 53: 4096-4102.