Cytokine effects on gap junction communication and connexin expression in human bladder smooth muscle cells and suburothelial myofibroblasts

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Heinrich, Marco ^a   Oberbach, Andreas ^b   Schlichting, Nadine ^b   Stolzenburg, Jens Uwe ^a   Neuhaus, Jochen ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b UNIVERSITY HOSPITAL LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 43; CONNEXIN 45; CYTOKINE; INTERLEUKIN 10; INTERLEUKIN 4; INTERLEUKIN 6; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR ALPHA; GAP JUNCTION PROTEIN;

ARTICLE; CELL INTERACTION; CONTROLLED STUDY; DOT HYBRIDIZATION; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GAP JUNCTION; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; MYOFIBROBLAST; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SMOOTH MUSCLE FIBER; UPREGULATION; ANIMAL; BLADDER; CELL COMMUNICATION; CELL CULTURE; CELL JUNCTION; CYTOLOGY; DRUG EFFECT; GENETICS; METABOLISM; MOUSE; OVERACTIVE BLADDER; PATHOLOGY; UROTHELIUM;

ANIMALS; CELL COMMUNICATION; CELLS, CULTURED; CONNEXIN 43; CONNEXINS; CYTOKINES; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GAP JUNCTIONS; GENE EXPRESSION REGULATION; HUMANS; MICE; MYOCYTES, SMOOTH MUSCLE; MYOFIBROBLASTS; RNA, MESSENGER; URINARY BLADDER; URINARY BLADDER, OVERACTIVE; UROTHELIUM;

EID: 79957964462     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020792     Document Type: Article

References (56)

1. Sui GP, Rothery S, Dupont E, Fry CH, Severs NJ, (2002) Gap junctions and connexin expression in human suburothelial interstitial cells. BJU Int 90: 118-129.
2. Kanai A, Roppolo J, Ikeda Y, Zabbarova I, Tai C, et al. (2007) Origin of spontaneous activity in neonatal and adult rat bladders and its enhancement by stretch and muscarinic agonists. Am J Physiol Renal Physiol 292: F1065-72.
3. Neuhaus J, Pfeiffer F, Wolburg H, Horn LC, Dorschner W, (2005) Alterations in connexin expression in the bladder of patients with urge symptoms. BJU Int 96: 670-676.
4. Neuhaus J, Heinrich M, Schwalenberg T, Stolzenburg JU, (2009) TGF-beta1 Inhibits Cx43 Expression and Formation of Functional Syncytia in Cultured Smooth Muscle Cells from Human Detrusor. Eur Urol 55: 491-498.
5. Haferkamp A, Mundhenk J, Bastian PJ, Reitz A, Dorsam J, et al. (2004) Increased expression of connexin 43 in the overactive neurogenic detrusor. Eur Urol 46: 799-805.
6. Roosen A, Datta SN, Chowdhury RA, Patel PM, Kalsi V, et al. (2009) Suburothelial Myofibroblasts in the Human Overactive Bladder and the Effect of Botulinum Neurotoxin Type A Treatment. Eur Urol 55: 1440-1448.
7. Erickson DR, Xie SX, Bhavanandan VP, Wheeler MA, Hurst RE, et al. (2002) A comparison of multiple urine markers for interstitial cystitis. J Urol 167: 2461-2469.
8. Ueda T, Tamaki M, Ogawa O, Yoshimura N, (2002) Over expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase in patients with interstitial cystitis and bladder carcinoma. J Urol 167: 347-351.
9. Tyagi P, Barclay D, Zamora R, Yoshimura N, Peters K, et al. (2009) Urine cytokines suggest an inflammatory response in the overactive bladder: a pilot study. Int Urol Nephrol.
10. Sant GR, Kempuraj D, Marchand JE, Theoharides TC, (2007) The mast cell in interstitial cystitis: role in pathophysiology and pathogenesis. Urology 69: 34-40.
11. Zhang H, Yang H, He S, (2010) TNF increases expression of IL-4 and PARs in mast cells. Cell Physiol Biochem 26: 327-336.
12. Bouchelouche K, Alvarez S, Horn T, Nordling J, Bouchelouche P, (2006) Human detrusor smooth muscle cells release interleukin-6, interleukin-8, and RANTES in response to proinflammatory cytokines interleukin-1beta and tumor necrosis factor-alpha. Urology 67: 214-219.
13. Saban R, Simpson C, Vadigepalli R, Memet S, Dozmorov I, et al. (2007) Bladder inflammatory transcriptome in response to tachykinins: neurokinin 1 receptor-dependent genes and transcription regulatory elements. BMC Urol 7: 7.
14. Oberbach A, Schlichting N, Bluher M, Kovacs P, Till H, et al. (2010) Palmitate induced IL-6 and MCP-1 expression in human bladder smooth muscle cells provides a link between diabetes and urinary tract infections. PLoS One 5: e10882.
15. Tacheau C, Laboureau J, Mauviel A, Verrecchia F, (2008) TNF-alpha represses connexin43 expression in HaCat keratinocytes via activation of JNK signaling. J Cell Physiol 216: 438-444.
16. Haghikia A, Ladage K, Lafenetre P, Haghikia A, Hinkerohe D, et al. (2008) Intracellular application of TNF-alpha impairs cell to cell communication via gap junctions in glioma cells. J Neurooncol 86: 143-152.
17. Lim MC, Maubach G, Zhuo L, (2009) TGF-beta1 down-regulates connexin 43 expression and gap junction intercellular communication in rat hepatic stellate cells. Eur J Cell Biol 88: 719-730.
18. Mori R, Power KT, Wang CM, Martin P, Becker DL, (2006) Acute downregulation of connexin43 at wound sites leads to a reduced inflammatory response, enhanced keratinocyte proliferation and wound fibroblast migration. J Cell Sci 119: 5193-5203.
19. Rama A, Matsushita T, Charolidi N, Rothery S, Dupont E, et al. (2006) Up-regulation of connexin43 correlates with increased synthetic activity and enhanced contractile differentiation in TGF-beta-treated human aortic smooth muscle cells. Eur J Cell Biol 85: 375-386.
20. Ahirwar D, Kesarwani P, Manchanda PK, Mandhani A, Mittal RD, (2008) Anti- and proinflammatory cytokine gene polymorphism and genetic predisposition: association with smoking, tumor stage and grade, and bacillus Calmette-Guerin immunotherapy in bladder cancer. Cancer Genet Cytogenet 184: 1-8.
21. Varambally S, Yu J, Laxman B, Rhodes DR, Mehra R, et al. (2005) Integrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression. Cancer Cell 8: 393-406.
22. Lampe PD, Lau AF, (2000) Regulation of gap junctions by phosphorylation of connexins. Arch Biochem Biophys 384: 205-215.
23. Mattey DL, Dawes PT, Nixon NB, Slater H, (1997) Transforming growth factor beta 1 and interleukin 4 induced alpha smooth muscle actin expression and myofibroblast-like differentiation in human synovial fibroblasts in vitro: modulation by basic fibroblast growth factor. Ann Rheum Dis 56: 426-431.
24. Zhang Y, Kanter EM, Laing JG, Aprhys C, Johns DC, et al. (2008) Connexin43 expression levels influence intercellular coupling and cell proliferation of native murine cardiac fibroblasts. Cell Commun Adhes 15: 289-303.
25. Crespin S, Bechberger J, Mesnil M, Naus CC, Sin WC, (2010) The carboxy-terminal tail of connexin43 gap junction protein is sufficient to mediate cytoskeleton changes in human glioma cells. J Cell Biochem 110: 589-597.
26. Rackauskas M, Neverauskas V, Skeberdis VA, (2010) Diversity and properties of connexin gap junction channels. Medicina (Kaunas) 46: 1-12.
27. Wang HZ, Brink PR, Christ GJ, (2006) Gap junction channel activity in short-term cultured human detrusor myocyte cell pairs: gating and unitary conductances. Am J Physiol Cell Physiol 291: C1366-76.
28. Eyden B, (2001) The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research. Ultrastruct Pathol 25: 39-50.
29. Muro AF, Chauhan AK, Gajovic S, Iaconcig A, Porro F, et al. (2003) Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan. J Cell Biol 162: 149-160.
30. Eyden B, (2004) Fibroblast phenotype plasticity: relevance for understanding heterogeneity in "fibroblastic" tumors. Ultrastruct Pathol 28: 307-319.
31. Drake MJ, Fry CH, Eyden B, (2006) Structural characterization of myofibroblasts in the bladder. BJU Int 97: 29-32.
32. Laird DW, (2006) Life cycle of connexins in health and disease. Biochem J 394: 527-543.
33. Kuijpers KA, Heesakkers JP, Jansen CF, Schalken JA, (2007) Cadherin-11 is Expressed in Detrusor Smooth Muscle Cells and Myofibroblasts of Normal Human Bladder. Eur Urol 52: 1213-1222.
34. Roosen A, Apostolidis A, Elneil S, Khan S, Panicker J, et al. (2009) Cadherin-11 up-regulation in overactive bladder suburothelial myofibroblasts. J Urol 182: 190-195.
35. Vandooren B, Cantaert T, ter Borg M, Noordenbos T, Kuhlman R, et al. (2008) Tumor necrosis factor alpha drives cadherin 11 expression in rheumatoid inflammation. Arthritis Rheum 58: 3051-3062.
36. Monahan TS, Andersen ND, Panossian H, Kalish JA, Daniel S, et al. (2007) A novel function for cadherin 11/osteoblast-cadherin in vascular smooth muscle cells: modulation of cell migration and proliferation. J Vasc Surg 45: 581-589.
37. Abbaci M, Barberi-Heyob M, Stines JR, Blondel W, Dumas D, et al. (2007) Gap junctional intercellular communication capacity by gap-FRAP technique: a comparative study. Biotechnol J 2: 50-61.
38. Wade MH, Trosko JE, Schindler M, (1986) A fluorescence photobleaching assay of gap junction-mediated communication between human cells. Science 232: 525-528.
39. Lampe PD, Lau AF, (2004) The effects of connexin phosphorylation on gap junctional communication. Int J Biochem Cell Biol 36: 1171-1186.
40. Lampe PD, TenBroek EM, Burt JM, Kurata WE, Johnson RG, et al. (2000) Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication. J Cell Biol 149: 1503-1512.
41. Kwak BR, Hermans MM, De Jonge HR, Lohmann SM, Jongsma HJ, et al. (1995) Differential regulation of distinct types of gap junction channels by similar phosphorylating conditions. Mol Biol Cell 6: 1707-1719.
42. Martin FJ, Prince AS, (2008) TLR2 regulates gap junction intercellular communication in airway cells. J Immunol 180: 4986-4993.
43. Kwak BR, Jongsma HJ, (1996) Regulation of cardiac gap junction channel permeability and conductance by several phosphorylating conditions. Mol Cell Biochem 157: 93-99.
44. Duthe F, Plaisance I, Sarrouilhe D, Herve JC, (2001) Endogenous protein phosphatase 1 runs down gap junctional communication of rat ventricular myocytes. Am J Physiol Cell Physiol 281: C1648-56.
45. Giepmans BN, Hengeveld T, Postma FR, Moolenaar WH, (2001) Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication. J Biol Chem 276: 8544-8549.
46. Meilleur MA, Akpovi CD, Pelletier RM, Vitale ML, (2007) Tumor necrosis factor-alpha-induced anterior pituitary folliculostellate TtT/GF cell uncoupling is mediated by connexin 43 dephosphorylation. Endocrinology 148: 5913-5924.
47. Fortin ME, Pelletier RM, Meilleur MA, Vitale ML, (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF. Biol Reprod 74: 2-12.
48. Neuhaus J, Schlichting N, Oberbach A, Stolzenburg JU, (2007) [Lipopolysaccharide-mediated regulation of interleukin-6 in cultured human detrusor smooth muscle cells.]. Urologe A 46: 1193-1197.
49. Bouchelouche K, Andresen L, Alvarez S, Nordling J, Nielsen OH, et al. (2006) Interleukin-4 and 13 Induce the Expression and Release of Monocyte Chemoattractant Protein 1, Interleukin-6 and Stem Cell Factor From Human Detrusor Smooth Muscle Cells: Synergy With Interleukin-1beta and Tumor Necrosis Factor-alpha. J Urol 175: 760-765.
50. Saban MR, O'Donnell MA, Hurst RE, Wu XR, Simpson C, et al. (2008) Molecular networks discriminating mouse bladder responses to intravesical bacillus Calmette-Guerin (BCG), LPS, and TNF-alpha. BMC Immunol 9: 4.
51. Shim JY, Park SW, Kim DS, Shim JW, Jung HL, et al. (2008) The effect of interleukin-4 and amphiregulin on the proliferation of human airway smooth muscle cells and cytokine release. J Korean Med Sci 23: 857-863.
52. Aoudjehane L, Pissaia AJ, Scatton O, Podevin P, Massault PP, et al. (2008) Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway. Lab Invest 88: 973-985.
53. Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, et al. (2007) Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells. Nat Immunol 8: 1372-1379.
54. Porter KE, Turner NA, O'Regan DJ, Ball SG, (2004) Tumor necrosis factor alpha induces human atrial myofibroblast proliferation, invasion and MMP-9 secretion: inhibition by simvastatin. Cardiovasc Res 64: 507-515.
55. Sharrow AC, Li Y, Micsenyi A, Griswold RD, Wells A, et al. (2008) Modulation of osteoblast gap junction connectivity by serum, TNFalpha, and TRAIL. Exp Cell Res 314: 297-308.
56. Akamine R, Yamamoto T, Watanabe M, Yamazaki N, Kataoka M, et al. (2007) Usefulness of the 5′ region of the cDNA encoding acidic ribosomal phosphoprotein P0 conserved among rats, mice, and humans as a standard probe for gene expression analysis in different tissues and animal species. J Biochem Biophys Methods 70: 481-486.