Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes

Plant OMICS

Volumn 4, Issue 1, 2011, Pages 29-33

  Yang, Chunxian ^a   Chen, Min ^a   Zeng, Lingjiang ^a   Zhang, Lei ^b   Liu, Xiaoqiang ^a   Lan, Xiaozhong ^c   Tang, Kexuan ^d   Liao, Zhihua ^a  

^a SOUTHWEST UNIVERSITY   (China)

^b SECOND MILITARY MEDICAL UNIVERSITY   (China)

^c Tibet Agricultural and Animal Husbandry College   (China)

^d SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Atropa Belladonna; H6H; Hairy Root; Metabolic Engineering; PMT; Tropane Alkaloids

Indexed keywords

ATROPA BELLADONNA;

EID: 79957921486     PISSN: 18360661     EISSN: 18363644     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Carvalho EB, Curtis WR. (1998) Characterization of fluid flow resistance in root cultures with a convective flow tubular bioreactor. Biotechnology and Bioengineering 60:375-384.
2. Dechaux C, Boitel-Conti M. (2005) A strategy for overaccumulation of scopolamine in Datura innoxia hairy root culture. Acta Biologica Coviensia Series Botanica 47: 101-107
3. Giri A, Narasu ML. (2000) Transgenic hairy roots: recent trends and applications. Biotechnology Advances 18:1-22
4. Guggisberg A, Hesse M. (1983) Putrescine, spermidine, spermine, andrelated polyamine alkaloids. In: Brossi A (ed) The Alkaloids, Academic Press, New York
5. Hashimoto T, Hayashi A, Amano Y, Kohno J, Iwanari H, Usuda S, Yamada Y. (1991) Hyoscyamine 6-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. Journal of Biological Chemistry 226: 4648-4653
6. Hibi N, Fujita T, Hatano M, Hashimoto T, Yamada Y. (1992) Putrescine N-methyltransferase in cultured roots of Hyoscyamus albus. Plant Physiology 100:826-835
7. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey KM. (1999) Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208: 545-551
8. Lan XZ, Quan H. (2010) Hairy root culture of Przewalskia tangutica for enhanced production of pharmaceutical tropane alkaloids. Journal of Medicinal Plants Research 4: 1477-481
9. Li W, Koike K, Asad Y, Yoshikaw T, Nikaido T. (2005) Biotransformation of paeonol by Panax ginseng root and cell cultures. Journal of Molecular Catalysis B: Enzymatic 5:117-121
10. Motomura K, Fujiwara Y, Kiyota N, Tsurushima K, Takeya M, Nohara T, Nagai R, Ikeda T. (2009) Astragalosides Isolated from the Root of Astragalus Radix Inhibit the Formation of Advanced Glycation End Products. Joural of Agricultural and Food Chemistry 57: 7666-7672
11. Moyano E, Fornalé S, Palazón J, Cusidó RM, Bagni B, Piñol MT. (2002) Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene. Phytochemistry 9: 697-702
12. Moyano E, Jouhikainen K, Tammela P, Palazón J, Cusidó RM, Piñol MT, Teeri TH, Oksman-Caldentey KM. (2003) Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. Journal of Experimental Botany 54: 203-211
13. Palazón J, Moyano E, Cusidó RM, Bonfill M, Oksman-Caldentey KM, Piñol MT. (2003) Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Science 165: 1289-1295
14. Rao AQ, Bakhsh A, Kiani S, Shahzad K, Shahid AA, Husnain T, Riazuddin S. (2009) The myth of plant transformation. Biotechnology Advances 27:753-763
15. Rothe G, Hachiya A, Yamada Y, Hashimoto T, Dräger B. (2003) Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine Nmethyltransferase. Journal of Experimental Botany 54: 2065-2070
16. Rothe G, Hachiya A, Yamada Y, Hashimoto T, Dräger B. (2003) Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase. Journal of Experimental Botany 54: 2065-2070
17. Yun DJ, Hashimoto T, Yamada Y. (1992) Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition. Proceedings of the National Academy of Sciences 24:11799-803
18. Zhang L, Ding RX, Chai YR, Bonfill M, Moyano E, Oksman-Caldentey KM, Xu TF,. Wang ZN Zhang HM, Kai GY, Liao ZH, Sun XF, Tang KX (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proceedings of the National Academy of Sciences 17:6786-6791
19. Zhang L, Kai GY, Lu BB, Zhang HM, Tang KX, Jiang JH, Chen WS. (2005) Metabolic engineering of tropane alkaloid biosynthesis in plants. Journal of Integrative Plant Biology 47:136-143
20. Zheng XY. (2005) Herba Belladonna. In: Zheng XY (ed) Pharmacopoeia of People's Republic of China, Beijing of China, 2005