Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus

Journal of Experimental Botany

Volumn 54, Issue 381, 2003, Pages 203-211

  Moyano, Elisabet ^a   Jouhikainen, Katja ^d   Tammela, Päivi ^d   Palazón, Javier ^b   Cusidó, Rosa M ^b   Piñol, M Teresa ^b   Teeri, Teemu H ^d   Oksman Caldentey, Kirsi Marja ^c  

^a UNIVERSITAT POMPEU FABRA   (Spain)

^b UNIVERSITY OF BARCELONA   (Spain)

^c VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^d NONE

Author keywords

35S pmt gene; Datura metel; Hairy root cultures; Hyoscyamine; Hyoscyamus muticus; Scopolamine; Tropane alkaloids

Indexed keywords

CATALYSIS; DRUG PRODUCTS; ENZYMES; GENETIC ENGINEERING; METABOLITES; PLANT CELL CULTURE;

GENETIC OVEREXPRESSION;

AMINO ACIDS;

DATURA METEL; EMBRYOPHYTA; EUKARYOTA; HYOSCYAMUS MUTICUS; NICOTIANA TABACUM;

EID: 0037300536     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/erg014     Document Type: Article

References (40)

1. Blondi S, Scaramagli S, Oksman-Caldentey K-M, Poli F. 2002. Secondary metabolism in root and callus cultures of Hyoscyamus multicus L.: the relationship between morphological organization and response to methyl jasmonate. Plant Science 163, 563-569.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
3. Celma RC, Palazón J, Cusidó RM, Piñol MT, Keil M. 2001. Decreased scopolamine yield in field-grown Duboisia plants regenerated from hairy roots. Planta Medica 67, 249-253.
4. Cusidó RM, Palazón J, Piñol MT, Bonfill M, Morales C. 1999. Datura metel: in vitro production of tropane alkaloids. Planta Medica 65, 144-148.
5. David C, Chilton MD, Tempé J. 1984. Conservation of T-DNA in plants regenerated from hairy root cultures. Bio/Technology 2, 73-76.
6. Edwards E, Johnstone C, Thompson A. 1991. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19, 1349.
7. Feth F, Arfmann H-A, Wray V, Wagner KG. 1985. Determination of putrescine N-methyltransferase by high performance liquid chromatography. Phytochemistry 24, 921-923.
8. Gamborg OL, Miller RA, Ojima K. 1986. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50, 151-158.
9. Hanahan D. 1983. Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166, 557-562.
10. Hashimoto T, Yamada Y. 1983. Scopolamine production in suspension cultures and rediffentiated roots of Hyoscyamus niger. Planta Medica 47, 121-141.
11. Hibi N, Fujita T, Hatano M, Hashimoto T, Yamada Y. 1992. Putrescine N-methyltransferase in cultured roots of Hyoscyamus albus. Plant Physiology 100, 826-835.
12. Hibi N, Higashiguchi S, Hashimoto T, Yamada Y. 1994. Gene expression in tobacco low-nicotine mutants. The Plant Cell 6, 723-735.
13. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey K-M. 1999. Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208, 545-551.
14. Kado CI, Liu ST. 1981. Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145, 1365-1373.
15. Kanegae T, Kajiya H, Amano Y, Hashimoto T, Yamada Y. 1994. Species-dependent expression of the hyoscyamine 6β-hydroxylase gene in the pericycle. Plant Physiology 105, 483-490.
16. Kholodenko BN, Cascante M, Hoek JB, Westerhoff HV, Schwaber J. 1998. Desired metabolite concentrations and fluxes. Biotechnology and Bioengineering 59, 239-247.
17. Maniatis T, Friotsh EF, Sambrook J. 1982. Molecular cloning, a laboratory manual. New York: Cold Spring Harbor Laboratory.
18. Martin-Tanguy J. 1997. Conjugated polyamines and reproductive development: biochemical, molecular and physiological approaches. Physiologia Plantarum 100, 675-688.
19. Miller JH. 1972. Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory.
20. Moyano E, Fornalé S, Palazón J, Cusidó RM, Bonfill M, Morales C, Piñol MT. 1999. Effect of Agrobacterium rhizogenes T-DNA on alkaloid production in Solanaceae plants. Phytochemistry 52, 1287-1292.
21. Mozo T, Hooykaas PJJ. 1991. Electroporation of megaplasmids into Agrobacterium. Plant Molecular Biology 16, 917-918.
22. Muranaka T, Ohkawa H, Yamada Y. 1993. Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system. Applied Microbiology and Biotechnology 40, 219-223.
23. Oksman-Caldentey K-M, Arroo R. 2000. Regulation of tropane alkaloid metabolism in plants and plant cell cultures. In: Verpoorte R, Alfermann AW, eds. Metabolic engineering of plant secondary metabolism. Dordrecht: Kluwer Academic Publishers, 253-281.
24. Oksman-Caldentey K-M, Kivelä O, Hiltunen R. 1991. Spontaneous shoot organogenesis and plant regeneration from hairy root cultures of Hyoscyamus muticus. Plant Science 78, 129-136.
25. Oksman-Caldentey K-M, Strauss A. 1986. Somaclonal variation of scopolamine content in protoplast-derived cell culture clones of Hyoscyamus muticus. Planta Medica 52, 6-12.
26. Oksman-Caldentey K-M, Vuorela H, Strauss A, Hiltunen R. 1987. Variation in the tropane alkaloid content of Hyoscyamus muticus plants and cell culture clones. Planta Medica 53, 349-354.
27. Palazón J, Altabella T, Cusidó RM, Ribó M, Piñol MT. 1995. Growth and tropane alkaloid production in Agrobacterium transformed roots and derived callus of Datura. Biologia Plantarum 37, 161-168.
28. Palazón J, Cusidó RM, Gonzalo J, Bonfill M, Morales C, Piñol MT. 1998. Relation between the amount of rolC gene product and indole alkaloid accumulation in Catharanthus roseus transformed root cultures. Journal of Plant Physiology 153, 712-718.
29. Piñol MT, Palazón J, Cusidó RM, Ribó M. 1999. Influence of calcium ion-concentration in the medium on tropane alkaloid accumulation in Datura stramonium hairy roots. Plant Science 141, 41-49.
30. Piñol MT, Palazón J, Cusidó RM, Serrano M. 1996. Effects of Ri T-DNA from Agrobacterium rhizogenes on growth and hyoscyamine production in Datura stramonium root cultures. Botanica Acta 109, 133-138.
31. Robins RJ, Bent FG, Rhodes MJC. 1991. Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 3. The relationship between morphological integrity and alkaloid biosynthesis. Planta 185, 390-395.
32. Sato F, Hashimoto T, Hachiya A, Tamura K, Choi K-B, Morishige T, Fujimoto H, Yamada Y. 2001. Metabolic engineering of plant alkaloid biosynthesis. Proceedings of the National Academy of Sciences, USA 98, 367-372.
33. Sevón N, Oksman-Caldentey K-M, Hiltunen R. 1995. Efficient plant regeneration from hairy root-derived protoplasts of Hyoscyamus muticus. Plant Cell Reports 14, 738-742.
34. Sevón N, Hiltunen R, Oksman-Caldentey K-M. 1998. Somaclonal variation in transformed root cultures and protoplast-derived hairy root cultures of Hyoscyamus muticus. Planta Medica 64, 37-41.
35. Suzuki K, Yamada Y, Hashimoto T. 1999. Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle. Plant Cell Physiology 40, 289-297.
36. Van Larebeke N, Engler G, Holsters M, Van den Esacker S, Schilperoort RA, Schell J. 1974. Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature 252, 169-170.
37. Vanhala L, Eeva M, Lapinjoki S, Hiltunen R, Oksman-Caldentey K-M. 1998. Effect of growth regulators on transformed root cultures of Hyoscyamus muticus. Journal of Plant Physiology 153, 475-481.
38. Vanhala L, Hiltunen R, Oksman-Caldentey K-M. 1995. Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus. Plant Cell Reports 14, 236-240.
39. White F, Nester EW. 1980. Hairy root: plasmid encodes virulence in Agrobacterium rhizogenes. Journal of Bacteriology 141, 1134-1141.
40. Yamada Y, Endo T. 1984. Tropane alkaloid production in cultured cells of Duboisia leichhardtii. Plant Cell Reports 3, 186-188.