Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene

Phytochemistry

Volumn 59, Issue 7, 2002, Pages 697-702

  Moyano, Elisabeth ^a   Fornalé, Silvia ^b   Palazón, Javier ^c   Cusidó, Rosa M ^c   Bagni, Nello ^b   Piñol, M Teresa ^c  

^a UNIVERSITAT POMPEU FABRA   (Spain)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Duboisia hybrid; Hairy roots; Hyoscyamine; Nicotine; Polyamines; Putrescine:SAM N methyltransferase; Scopolamine; Solanaceae

Indexed keywords

ALKALOID; KANAMYCIN; METHYLTRANSFERASE; PUTRESCINE; PUTRESCINE DERIVATIVE; PUTRESCINE N METHYLTRANSFERASE; PUTRESCINE N-METHYLTRANSFERASE; SCOPOLAMINE;

AGROBACTERIUM TUMEFACIENS; ANGIOSPERM; ARTICLE; BIOSYNTHESIS; CATALYST; CHEMICAL COMPOSITION; CHIMERA; CONTROLLED STUDY; DUBOISIA; ENZYME ACTIVITY; ENZYMOLOGY; GENE CONTROL; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HAIRY ROOT CULTURE; HYBRID; METABOLISM; METHYLATION; NONHUMAN; NORTHERN BLOTTING; PLANT ROOT; POLYMERASE CHAIN REACTION; PROMOTER REGION; TISSUE CULTURE; TOBACCO; TRANSGENIC PLANT; CULTURE TECHNIQUE; GROWTH, DEVELOPMENT AND AGING; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; DUBOISIA; NICOTIANA; NICOTIANA TABACUM; SOLANACEAE;

ALKALOIDS; CHIMERA; CULTURE TECHNIQUES; GENE EXPRESSION; METHYLTRANSFERASES; PLANT ROOTS; PUTRESCINE; RHIZOBIUM RADIOBACTER; SOLANACEAE; TRANSFECTION;

EID: 0037041051     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0031-9422(02)00044-4     Document Type: Article

References (26)

1. Datura metel: In vitro production of tropane alkaloids
2. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
3. Alkaloid production in cultured roots of three species of Duboisia
4. Nutrient requirements of suspension cultures of soybean root cells
5. Putrescine, spermidine, spermine, and related polyamine alkaloids
6. Studies on transformation of Escherichia coli with plasmids
7. Production of tropane alkaloids in genetically engineered root cultures
8. Putrescine and putrescine N-methyltransferase in the biosynthesis of tropane alkaloids in cultured roots of Hyocyamus albus I. Biochemical studies
9. Gene expression in tobacco low-nicotine mutants
10. Growth and hyoscyamine production of hairy root cultures of Datura stramonium in a modified stirred tank reactor
11. Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures
12. Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering
13. Effect of Agrobacterium rhizagenes T-DNA on alkaloid production in Solanaceae plants
14. Effect of pmt gene overexpresion on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus
15. Electroporation of megaplasmids into Agrobacterium
16. Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system
17. A revised medium for rapid growth and bioassays with tobacco tissue cultures
18. Regulation of tropane alkaloid metabolism in plants and plant cell cultures
19. Growth and tropane alkaloid production in Agrobacterium transformed roots and derived callus of Datura
20. Effects of Ri T-DNA from Agrobacterium rhizogenes on growth and hyoscyamine production in Datura stramonium root cultures
21. Influence of calcium ion-concentration in the medium on tropane alkaloid accumulation in Datura stramonium hairy roots
22. Studies on the biosynthesis of tropane alkaloids in Datura stramonium L. transformed root cultures. 2. On the relative contribution of L-arginine and L-ornithine to the formation of the tropane ring
23. Metabolic engineering of plant alkaloid biosynthesis
24. Quantitation of major tobacco alkaloids by high-performance liquid chromatography
25. Polyamine content in Arabidopsis thaliana and effect of spermidine on plant development