In vitro micronucleus screening of pharmaceutical candidates by flow cytometry in Chinese hamster V79 cells

Environmental and Molecular Mutagenesis

Volumn 52, Issue 5, 2011, Pages 355-362

  Nicolette, John ^a   Diehl, Marilyn ^a   Sonders, Paul ^a   Bryce, Steve ^b   Blomme, Eric ^a  

^a ABBOTT LABORATORIES   (United States)

^b LITRON LABORATORIES   (United States)

Author keywords

Drug discovery; Flow cytometry; Micronucleus; Microscopy; V79 cells

Indexed keywords

ANEUGEN; CLASTOGEN; DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; AUTOMATION; CELL STRAIN V 79; CONTROLLED STUDY; CYTOKINESIS; DNA CONTENT; EVALUATION; FLOW CYTOMETRY; GENOTOXICITY; HUMAN; IMMUNOLOGY; IN VITRO STUDY; INTERMETHOD COMPARISON; MEDICAL RESEARCH; MICRONUCLEUS; MICROSCOPY; NEUROSCIENCE; NONHUMAN; ONCOLOGY; PHARMACEUTICS; SCREENING;

ANIMALS; CELL LINE; CRICETINAE; CRICETULUS; DRUG DISCOVERY; FLOW CYTOMETRY; MICRONUCLEUS TESTS;

CRICETULUS GRISEUS;

EID: 79957904069     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.20631     Document Type: Article

References (20)

1. Ames B, McCann J, Yamasaki E. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat Res 31: 347-364.
2. Avlasevich SL, Bryce SM, Cairns SE, Dertinger SD. 2006. In vitro micronucleus scoring by flow cytometry: Differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ Mol Mutagen 47: 56-66.
3. Bryce SM, Bemis JC, Avlasevich S, Dertinger SD. 2007. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity. Mutat Res 630: 78-91.
4. Bryce SM, Shi J, Nicolette J, Diehl M, Sonders P, Avlasevich S, Raja S, Bemis JC, Dertinger SD. 2010. High content flow cytometric micronucleus scoring method is applicable to attachment cell lines. Environ Mol Mutagen 51: 260-266.
5. Decordier I, Papine A, Plas G, Roesems S, Vande Loock K, Moreno-Paloma J, Cemeli E, Anderson D, Fucic A, Marcos R, Soussaline F, Kirsch-Volders M. 2009. Automated image analysis of cytokinesis-blocked micronuclei: An adapted protocol and a validated scoring procedure. Mutagen 24: 85-93.
6. Diaz D, Scott A, Carmichael P, Shi W, Costales C. 2007. Evaluation of an automated in vitro micronucleus assay in CHO-K1 cells. Mutat Res 630: 1-13.
7. Eastmond D, Tucker J. 1989. Kinetochore localization in micronucleated cytokinesis-blocked Chinese hamster ovary cells: A new and rapid assay for identifying aneuploidy-inducing agents. Mutat Res 224: 517-525.
8. Elhajouji A, Van Hummelen P, Kirsch-Volders M. 1995. Indications for a threshold of chemically-induced aneuploidy in vitro in human lymphocytes. Environ Mol Mutagen 26: 292-304.
9. Elhajouji A, Tibaldi F, Kirsch-Volders M. 1997. Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes. Mutagen 12: 133-140.
10. Fenech M, Morley A. 1988. Preliminary studies on scoring micronuclei by computerised image analysis. Mutat Res 203: 33-38.
11. Fenech M. 2000. The in vitro micronucleus technique. Mutat Res 455: 81-95.
12. Fenech M. 2006. Commentary on the SFTG international collaboration study on the in vitro micronucleus test: to Cyt-B or not to Cyt-B? Mutat Res 607: 9-12.
13. Hashimoto K, Nakajima Y, Matsumura S, Chatani F. 2010. An in vitro micronucleus assay with size-classified micronucleus counting to discriminate aneugens from clastogens. Toxicol In Vitro 24: 208-216.
14. Krishna G, Fiedler R, Theiss J. 1992. Simultaeous analysis of chromosome damage and aneuploidy in cytokinesis-blocked V79 Chinese hamster lung cells using antikinetochore antibody. Mutat Res 282: 79-88.
15. Miller B, Nusse M. 1993. Analysis of micronuclei induced by 2-chlorobenzylidene malonitrile (CS) using fluorescence in situ hybridization with telomeric and centromeric DNA probes, and flow cytometry. Mutagen 8: 35-41.
16. Nusse M, Kramer J. 1984. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 5: 20-25.
17. Organization for Economic Co-operation and Development (OECD). 2007. Draft Guideline 487. In vitro Mammalian Cell Micronucleus Test, 3rd Version.
18. Parry JM, Parry EM. 2006. The use of the in vitro micronucleus assay to detect and assess the aneugenic activity of chemicals. Mutat Res 607: 5-8.
19. Roman D, Locher F, Suter W, Cordier A, Bobadilla M. 1998. Evaluation of a new procedure for the flow cytometric analysis of in vitro, chemically induced micronuclei in V79 cells. Environ Mol Mutagen 32: 387-396.
20. Shi J, Bezabhie R, Szkudlinska A. 2010. Further evaluation of a flow cytometric in vitro micronucleus assay in CHO-K1 cells: A reliable platform that detects micronuclei and discriminates apoptotic bodies. Mutagen 25: 33-40.