Neurofilament dot blot assays: Novel means of assessing axon viability in culture

Journal of Neuroscience Methods

Volumn 198, Issue 2, 2011, Pages 195-203

  Hares, Kelly ^a   Kemp, Kevin ^a   Gray, Elizabeth ^a   Scolding, Neil ^a   Wilkins, Alastair ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Axon viability; Dot blot assay; Neurodegenerative disease; Neurofilament; Phosphorylation

Indexed keywords

GLIAL CELL LINE DERIVED NEUROTROPHIC FACTOR; NITRIC OXIDE; NITRIC OXIDE DONOR; SUPEROXIDE DISMUTASE;

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL CULTURE; CELL VIABILITY; CYTOTOXICITY; DOT HYBRIDIZATION; EMBRYO; IMMUNOCYTOCHEMISTRY; NERVE FIBER; NEUROFILAMENT; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; RAT;

ANIMALS; AXONS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CEREBRAL CORTEX; IMMUNOBLOTTING; INTERMEDIATE FILAMENTS; MICROFILAMENTS; NEUROFILAMENT PROTEINS; NEURONS; PHOSPHORYLATION; RATS;

EID: 79957711784     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.03.025     Document Type: Article

References (37)

1. Ahmed Z., Gveric D., Pryce G., Baker D., Leonard J.P., Cuzner M.L., et al. Myelin/axonal pathology in interleukin-12 induced serial relapses of experimental allergic encephalomyelitis in the Lewis rat. Am J Pathol 2001, 158:2127-2138.
2. Black M.M., Lasek R.J. Slow components of axonal transport: two cytoskeletal networks. J Cell Biol 1980, 86:616-623.
3. Brownlees J., Ackerley S., Grierson A.J., Jacobsen N.J., Shea K., Anderton B.H., et al. Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum Mol Genet 2002, 11:2837-2844.
4. Carden M.J., Trojanowski J.Q., Schlaepfer W.W., Lee V.M. Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns. J Neurosci 1987, 7:3489-3504.
5. Cote F., Collard J.F., Julien J.P. Progressive neuronopathy in transgenic mice expressing the human neurofilament heavy gene: a mouse model of amyotrophic lateral sclerosis. Cell 1993, 73:35-46.
6. Elhanany E., Jaffe H., Link W.T., Sheeley D.M., Gainer H., Pant H.C. Identification of endogenously phosphorylated KSP sites in the high-molecular-weight rat neurofilament protein. J Neurochem 1994, 63:2324-2335.
7. Ferguson B., Matyszak M.K., Esiri M.M., Perry V.H. Axonal damage in acute multiple sclerosis lesions. Brain 1997, 120(Pt 3):393-399.
8. Finn J.T., Weil M., Archer F., Siman R., Srinivasan A., Raff M.C. Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases. J Neurosci 2000, 20:1333-1341.
9. Goldstein M.E., Sternberger N.H., Sternberger L.A. Phosphorylation protects neurofilaments against proteolysis. J Neuroimmunol 1987, 14:149-160.
10. Iannotti C., Li H., Yan P., Lu X., Wirthlin L., Xu X.M. Glial cell line-derived neurotrophic factor-enriched bridging transplants promote propriospinal axonal regeneration and enhance myelination after spinal cord injury. Exp Neurol 2003, 183:379-393.
11. Keller J.N., Mark R.J., Bruce A.J., Blanc E., Rothstein J.D., Uchida K., et al. 4-Hydroxynonenal, an aldehydic product of membrane lipid peroxidation, impairs glutamate transport and mitochondrial function in synaptosomes. Neuroscience 1997, 80:685-696.
12. Kemp K., Gray E., Mallam E., Scolding N., Wilkins A. Inflammatory cytokine induced regulation of superoxide dismutase 3 expression by human mesenchymal stem cells. Stem Cell Rev 2010, 6:548-559.
13. Kemp K., Hares K., Mallam E., Heesom K.J., Scolding N., Wilkins A. Mesenchymal stem cell-secreted superoxide dismutase promotes cerebellar neuronal survival. J Neurochem 2010, 114:1569-1580.
14. Lee M.K., Cleveland D.W. Neuronal intermediate filaments. Annu Rev Neurosci 1996, 19:187-217.
15. Mattson M.P., Liu D. Energetics and oxidative stress in synaptic plasticity and neurodegenerative disorders. Neuromolecular Med 2002, 2:215-231.
16. Motil J., Chan W.K., Dubey M., Chaudhury P., Pimenta A., Chylinski T.M., et al. Dynein mediates retrograde neurofilament transport within axons and anterograde delivery of NFs from perikarya into axons: regulation by multiple phosphorylation events. Cell Motil Cytoskeleton 2006, 63:266-286.
17. Murayama S., Bouldin T.W., Suzuki K. Immunocytochemical and ultrastructural studies of upper motor neurons in amyotrophic lateral sclerosis. Acta Neuropathol 1992, 83:518-524.
18. Neely M.D., Sidell K.R., Graham D.G., Montine T.J. The lipid peroxidation product 4-hydroxynonenal inhibits neurite outgrowth, disrupts neuronal microtubules, and modifies cellular tubulin. J Neurochem 1999, 72:2323-2333.
19. Nixon R.A., Paskevich P.A., Sihag R.K., Thayer C.Y. Phosphorylation on carboxyl terminus domains of neurofilament proteins in retinal ganglion cell neurons in vivo: influences on regional neurofilament accumulation, interneurofilament spacing, and axon caliber. J Cell Biol 1994, 126:1031-1046.
20. Pazyra-Murphy M.F., Segal R.A. Preparation and maintenance of dorsal root ganglia neurons in compartmented cultures. J Vis Exp 2008.
21. Perez-Olle R., Lopez-Toledano M.A., Goryunov D., Cabrera-Poch N., Stefanis L., Brown K., et al. Mutations in the neurofilament light gene linked to Charcot-Marie-Tooth disease cause defects in transport. J Neurochem 2005, 93:861-874.
22. Perrot R., Berges R., Bocquet A., Eyer J. Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration. Mol Neurobiol 2008, 38:27-65.
23. Petzold A. Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss. J Neurol Sci 2005, 233:183-198.
24. Semra Y.K., Seidi O.A., Sharief M.K. Heightened intrathecal release of axonal cytoskeletal proteins in multiple sclerosis is associated with progressive disease and clinical disability. J Neuroimmunol 2002, 122:132-139.
25. Shea T.B., Chan W.K. Regulation of neurofilament dynamics by phosphorylation. Eur J Neurosci 2008, 27:1893-1901.
26. Smith K.J., Lassmann H. The role of nitric oxide in multiple sclerosis. Lancet Neurol 2002, 1:232-241.
27. Stevenson A., Yates D.M., Manser C., De Vos K.J., Vagnoni A., Leigh P.N., et al. Riluzole protects against glutamate-induced slowing of neurofilament axonal transport. Neurosci Lett 2009, 454:161-164.
28. Trapp B.D., Peterson J., Ransohoff R.M., Rudick R., Mork S., Bo L. Axonal transection in the lesions of multiple sclerosis. N Engl J Med 1998, 338:278-285.
29. Veeranna, Amin N.D., Ahn N.G., Jaffe H., Winters C.A., Grant P., et al. Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M. J Neurosci 1998, 18:4008-4021.
30. Wilkins A., Chandran S., Compston A. A role for oligodendrocyte-derived IGF-1 in trophic support of cortical neurons. Glia 2001, 36:48-57.
31. Wilkins A., Compston A. Trophic factors attenuate nitric oxide mediated neuronal and axonal injury in vitro: roles and interactions of mitogen-activated protein kinase signalling pathways. J Neurochem 2005, 92:1487-1496.
32. Wilkins A., Kemp K., Ginty M., Hares K., Mallam E., Scolding N. Human bone marrow-derived mesenchymal stem cells secrete brain-derived neurotrophic factor which promotes neuronal survival in vitro. Stem Cell Res 2009.
33. Wilkins A., Majed H., Layfield R., Compston A., Chandran S. Oligodendrocytes promote neuronal survival and axonal length by distinct intracellular mechanisms: a novel role for oligodendrocyte-derived glial cell line-derived neurotrophic factor. J Neurosci 2003, 23:4967-4974.
34. Wilkins A., Scolding N. Protecting axons in multiple sclerosis. Mult Scler 2008, 14:1013-1025.
35. Yabe J.T., Chylinski T., Wang F.S., Pimenta A., Kattar S.D., Linsley M.D., et al. Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites. J Neurosci 2001, 21:2195-2205.
36. Yabe J.T., Wang F.S., Chylinski T., Katchmar T., Shea T.B. Selective accumulation of the high molecular weight neurofilament subunit within the distal region of growing axonal neurites. Cell Motil Cytoskeleton 2001, 50:1-12.
37. Zhang L., Ma Z., Smith G.M., Wen X., Pressman Y., Wood P.M., et al. GDNF-enhanced axonal regeneration and myelination following spinal cord injury is mediated by primary effects on neurons. Glia 2009, 57:1178-1191.