Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

The Lancet

Volumn 375, Issue 9710, 2010, Pages 224-230

  Tissari, Päivi ^a   Zumla, Alimuddin ^b   Tarkka, Eveliina ^a   Mero, Sointu ^a   Savolainen, Laura ^a   Vaara, Martti ^a   Aittakorpi, Anne ^c   Laakso, Sanna ^c   Lindfors, Merja ^c   Piiparinen, Heli ^c   Mäki, Minna ^c   Carder, Caroline ^b   Huggett, Jim ^b   Gant, Vanya ^b  

^a HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

^b UNIVERSITY COLLEGE LONDON HOSPITALS   (United Kingdom)

^c Mobidiag Ltd   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTEREMIA; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; DIAGNOSTIC ACCURACY; DNA MICROARRAY; FINLAND; GENE AMPLIFICATION; HUMAN; MAJOR CLINICAL STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; OBSERVATIONAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEPSIS; SPECIES IDENTIFICATION; STAPHYLOCOCCUS INFECTION; UNITED KINGDOM;

EID: 74249100609     PISSN: 01406736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0140-6736(09)61569-5     Document Type: Article

References (23)

1. Angus D.C., and Wax R.S. Epidemiology of sepsis: an update. Crit Care Med 29 suppl 7 (2001) 109-116
2. Zaidi A.K., Thaver D., Ali S.A., and Khan T.A. Pathogens associated with sepsis in newborns and young infants in developing countries. Pediatr Infect Dis J 28 suppl 1 (2009) 10-18
3. Howard D., Cordell R., McGowan Jr. J.E., Packard R.M., Scott II R.D., Solomon S.L., and Workshop group. Measuring the economic costs of antimicrobial resistance in hospital settings: summary of the Centers for Disease Control and Prevention-Emory workshop. Clin Infect Dis 33 (2001) 1573-1578
4. Andrade S.S., Bispo P.J., and Gales A.C. Advances in the microbiological diagnosis of sepsis. Shock 30 suppl 1 (2008) 41-46
5. Davey P.G., and Marwick C. Appropriate vs inappropriate antimicrobial therapy. Clin Microbiol Infect 14 suppl 3 (2008) 15-21
6. Roth S.B., Jalava J., Ruuskanen O., Ruholoa A., and Nikkari S. Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections. J Clin Microbiol 42 (2004) 4268-4274
7. Eigner U., Weizenegger M., Fahr A.M., and Witte W. Evaluation of a rapid direct assay for identification of bacteria and the mec A and van genes from positive-testing blood cultures. J Clin Microbiol 43 (2005) 5256-5262
8. CLSI. Performance standards for antimicrobial disk susceptibility tests; approved standard, document M2-A9, 2003). 8th edn Vols 23 (2003), Clinical and Laboratory Standards Institute, Wayne, PA, USA and 26 (9th edn, 2006)
9. European Committee for Standardisation. BS EN 13612:2002 Performance evaluation of in vitro diagnostic medical devices (2002), British Standards Institute, London
10. Wayne P.A. CLSI. User protocol for evaluation of qualitative test performance. Document EP12-A (2002), CLSI, Wayne, PA, USA
11. Vince A., Lepej S.Z., Barsic B., et al. Light Cycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy. J Med Microbiol 57 (2008) 1306-1307
12. Gerbert S., Siegel D., and Wellinghausen N. Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis. J Infect 57 (2008) 307-316
13. Reier-Nilsen T., Farstad T., Nakstad B., Lauvrak V., and Steinbakk M. Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study. BMC Pediatr 19 (2009) 5
14. West H., Lisby G., Breysse F., et al. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15 (2009) 544-551
15. Lehmann L.E., Hunfeld K.P., Emrich T., et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 197 (2008) 313-324
16. Paule S.M., Hacek D.M., Kufner B., et al. Performance of the BD GeneOhm meticillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J Clin Microbiol 45 (2007) 2993-2998
17. Mancini N., Carletti S., Ghidoli N., et al. Molecular diagnosis of polymicrobial sepsis. J Clin Microbiol 47 (2009) 1274-1275
18. Cenciarining-Borde C., Courtois S., and La Scola B. Nucleic acids as viability markers for bacteria detection using molecular tools. Future Micrbiol 4 (2009) 45-64
19. Odds F.C., Hanson M.F., Davidson A.D., et al. One year prospective survey of Candida bloodstream infections in Scotland. J Med Microbiol 56 (2007) 1066-1075
20. Borrás Salvador R., Cuenca-Estrella M., Domínguez Márquez M.V., and Gadea Gironés I. Molecular diagnosis of parasitic and fungal infections. Enferm Infecc Microbiol Clin 26 suppl 9 (2008) 50-57
21. Kompalic-Cristo A., Frotta C., Suárez-Mutis M., Fernandes O., and Britto C. Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res 101 (2007) 619-625
22. Deborggraeve S., Laurent T., Espinosa D., et al. A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis. J Infect Dis 198 (2008) 1565-1572
23. Mens P., Spieker N., Omar S., Heijnen M., Schallig H., and Kager P.A. Is molecular biology the best alternative for diagnosis of malaria to microscopy? A comparison between microscopy, antigen detection and molecular tests in rural Kenya and urban Tanzania. Trop Med Int Health 12 (2007) 238-244